Phenolics, fatty acids composition and biological activities of various extracts and fractions of Malaysian Aaptos aaptos

Rashid, Zalilawati Mat; Ali, Abdul Manaf; Douzenel, Philippe; Bourgougnon, Nathalie; Shaari, Khozirah; Andriani, Yosie; Muhammad, Tengku Sifzizul Tengku; Mohamad, Habsah

doi:10.4103/2221-1691.245971

Cited by 9 publications

(3 citation statements)

References 55 publications

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“…, 2014 ; Lopes & Bernardo-Gil, 2005 ; Rashid et al . , 2018 ). Given that the fatty acid sources used for SL production are usually plant-derived sources that are rich in fatty acids of differing carbon length and saturation and form, it is likely that oil-based components are co-extracted with the SLs in the ethyl acetate step.…”

Section: Discussionmentioning

confidence: 99%

Determining the accuracy and suitability of common analytical techniques for sophorolipid biosurfactants

Ingham,

Sung,

Kay

et al. 2024

Journal of Industrial Microbiology and Biotechnology

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To determine the performance of a sophorolipid biosurfactant production process, it is important to have accurate and specific analytical techniques in place. Among the most popular are the anthrone assay, gravimetric quantification (hexane:ethyl acetate extraction), and high-performance liquid chromatography (HPLC). The choice of analytical tool varies depending on cost, availability, and ease of use; however, these techniques have never been compared directly against one another. In this work, 75 fermentation broths with varying product/substrate concentrations were comprehensively tested with the 3 techniques and compared. HPLC–ultraviolet detection (198 nm) was capable of quantifying C18:1 subterminal hydroxyl diacetylated lactonic sophorolipid down to a lower limit of 0.3 g/L with low variability (<3.21%). Gravimetric quantification of the broths following liquid:liquid extraction with hexane and ethyl acetate showed some linearity (R2 = .658) when compared to HPLC but could not quantify lower than 11.06 g/L, even when no sophorolipids were detected in the sample, highlighting the non-specificity of the method to co-extract non-sophorolipid components in the final gravimetric measure. The anthrone assay showed no linearity (R2 = .129) and was found to cross-react with media components (rapeseed oil, corn steep liquor, glucose), leading to consistent overestimation of sophorolipid concentration. The appearance of poor biomass separation during sample preparation with centrifugation was noted and resolved with a novel sample preparation method with pure ethanol. Extensive analysis and comparisons of the most common sophorolipid quantification techniques are explored and the limitations/advantages are highlighted. The findings provide a guide for scientists to make an informed decision on the suitable quantification tool that meets their needs, exploring all aspects of the analysis process from harvest, sample preparation, and analysis.

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Section: Discussionmentioning

confidence: 99%

Determining the accuracy and suitability of common analytical techniques for sophorolipid biosurfactants

Ingham,

Sung,

Kay

et al. 2024

Journal of Industrial Microbiology and Biotechnology

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“…New metabolites from plants are discovered every year in an attempt to discover new antimicrobial compounds due to the high rate of resistance of microorganisms to standard antibiotics [1].…”

Section: Introductionmentioning

confidence: 99%

“…Fatty acids are molecules typically found attached to other compounds such as sugars, glycerol or phosphate head groups to form lipids [2] and many are known to have originated from unusual biosynthetic pathways, thus displaying unusual characteristic of unsaturated patterns, exhibit terminal and/or mid-chain branching [1]. Lipids are essential components of cell structures like the cell membranes, which are main components of phospholipids, and energy stores that are often composed of triglycerides.…”

Section: Introductionmentioning

confidence: 99%

Fatty acid profile, antioxidant and antibacterial effect of the ethyl acatate extract of cleistopholis patens

A.O

Temikotan

2021

Bull. Sci. Res.

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Cleistopholis patens is a tropical plant that is used in the treatment of many bacterial and fungal infections. In this study, the antibacterial action against some human pathogens and the fatty acid profile of the plant were investigated. The plant was found to be active against Salmonella typhi Streptococcus pyogenes, Staphylococcus aureus while Shigella dysenteriae was resistant. The comparative antibiotic test revealed that only Staphylococcus aureus was sensitive to chloramphenicol and gentamycin. The purified extract showed lesser activity than the crude extract. Phytochemical components include; glycosides, steroids, Phenol, tanins and saponins. Anticonstituents include tanin (2.32mg/g), phenol (2.50 mg/g), phytate (15.65 mg/g), oxalate (6.57 mg/g), saponin (9.71 mg/g) and flavonoids (6.49 mg/g). The GCMS profile of the ethyl acetate extract of the plant revealed 23 fatty acid including 9-Hexadecanoic acid, (25.11%), 6 octadecanoic acid (21.98%), n Hexadecanoic (4.62%), Cyclopentaneundecanoic acid, methyl ester (2.05%). Heptacosanoic acid, methyl ester (2.05%), Decanoic acid, methyl ester (1.74%), Oleic acid (4.38%), 16-Octadecenoic acid, methyl ester (3.55%) amongst others.

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Studies of chemical distribution and pharmacological activities of porifera-derived alkaloids: A review (2000–2023)

Khatun,

Hazra,

Mahato

et al. 2024

European Journal of Medicinal Chemistry Reports

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Phenolics, fatty acids composition and biological activities of various extracts and fractions of Malaysian Aaptos aaptos

Cited by 9 publications

References 55 publications

Determining the accuracy and suitability of common analytical techniques for sophorolipid biosurfactants

Determining the accuracy and suitability of common analytical techniques for sophorolipid biosurfactants

Fatty acid profile, antioxidant and antibacterial effect of the ethyl acatate extract of cleistopholis patens

Studies of chemical distribution and pharmacological activities of porifera-derived alkaloids: A review (2000–2023)

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