Phenotype and chondrogenic differentiation of mesenchymal cells from adipose tissue of different species

Abstract

Mesenchymal stem cells (MSCs) are multipotent cells capable of differentiating into several mesoderm lineages. They have been isolated from different tissues, such as bone marrow, adult peripheral blood, umbilical cord blood, and adipose tissue. The aim of this study was to analyze the differences in proliferation and phenotype of adipose tissue-derived MSCs from three different species, and to evaluate their capacity to differentiate into chondrocytes in vitro. A comparative study of cultured human, rabbit, and sheep mesenchymal cells from adipose tissue was carried out, and the main morphological parameters, proliferative activity, and expression of surface markers were characterized. Proliferation and flow cytometry data showed species-related differences between animal and human MSCs. Histological staining suggested that rabbit and sheep mesenchymal cells were able to differentiate into chondrocytic lineages. Human mesenchymal cells, though they could also differentiate, accomplished it with more difficulty than animal MSCs. These results could help to explain the differences in the chondrogenic capacity of sheep and rabbit MSCs when they are used as animal models compared to human mesenchymal cells in a clinical assay. ß

A horse of a different color

“…This result agrees with the findings of others in equine MSCs (9), being coexpressed by more than the 92% of the cells (10). A high percentage of MSCs from other species such as canine, ovine, or porcine also display in their cell surface these molecules (11)(12)(13).…”

Isolation, culture and characterization of multipotent mesenchymal stem cells from goat umbilical cord blood

“…CD44 is a hyaluronate receptor. The positive staining for CD44 antibody in flow cytometry argued with that published by Barry et al (1999) and Martínez et al (2009). These studies showed that CD44 is an antibody that characterizes mesenchymal cells for human and sheep fat tissue derived cells.…”

Comparative immunophenotyping of equine multipotent mesenchymal stromal cells: An approach toward a standardized definition

“…As in equine MSCs, CD 29 and CD 44 expressions are found consistently in canine (34,35,51,52), ovine (53), and porcine (54) MSCs. Corresponding to the equine antigen expression profile as well, CD 73 could not be detected in canine, rabbit, or sheep MSCs (35,55,56), while rat tendon derived, feline and bovine neonatal cell sources and caprine bone marrow-derived MSCs expressed this antigen (57)(58)(59)(60). CD 73 protein expression could also be detected in bone marrow derived MSCs from mice but only in moderate amounts (61).…”