Phenotype and Genotype of Pancreatic Cancer Cell Lines

Abstract

The dismal prognosis of pancreatic adenocarcinoma (PA) is due in part due to a lack of molecular information regarding disease development. Established cell lines remain a useful tool for investigating these molecular events. Here we present a review of available information on commonly used PA cell lines as a resource to help investigators select the cell lines most appropriate for their particular research needs. Information on clinical history, in vitro and in vivo growth characteristics, phenotypic characteristics, such as adhesion, invasion, migration and tumorigenesis, and genotypic status of commonly altered genes (KRAS, p53, p16, and SMAD4) was evaluated. Identification of both consensus and discrepant information in the literature suggests careful evaluation before selection of cell lines and attention be given to cell line authentication.

MIST1 Links Secretion and Stress as both Target and Regulator of the Unfolded Protein Response

“…Because MIST1 is restricted to professional secretory cells (e.g., acinar cells), we asked if nonsecretory cells could similarly induce Mist1 expression upon ER stress. For these studies, we utilized a number of human pancreatic ductal cell lines that normally do not express Mist1 (14,20,25,40,41). In all cases, treatment of cells with thapsigargin led to induction of Xbp1s and Mist1 transcripts ( Fig.…”

Natural killer cells as effectors of selection and differentiation of stem cells: Role in resolution of inflammation

“…To assess whether the stage of differentiation of other tumor types also correlated with their sensitivity to NK cell-mediated lysis, five pancreatic lines at different stages of differentiation were selected based on a number of criteria including sphere formation and immunohistochemical analysis (Sipos, 2003;Deer, 2010). In these studies , Panc-1 and MP-2 (two poorly-differentiated), BXPC3 and HPAF (two moderatelydifferentiated), and CAPAN-1 (a well-differentiated) pancreatic tumor cell lines were co-cultured with the NK cells and NKmediated cytotoxicity was determined in a 4-h [ 51 Cr] release assay.…”

Multifunctional, biocompatible and pH-responsive carbon nanotube- and graphene oxide/tectomer hybrid composites and coatings

“…102 PANC-1 and BxPC-3 are derived from a primary tumour, and AsPC-1 originates from ascites (metastasis). 103 Each cell line has an epithelial morphology and the adherent cells form a monolayer in culture. As can be seen in Fig.…”