Phenotype differentiation of three E. coli strains by GC-FID and GC–MS based metabolomics

Enhancement of riboflavin production with Bacillus subtilis by expression and site-directed mutagenesis of zwf and gnd gene from Corynebacterium glutamicum

“…Intracellular metabolome analysis is a good option for detecting the genetic modification effects. Tian et al (2008) performed the intracellular metabolome analysis of two mutants of E. coli with deletion of sdhAB and ackA-pta genes, respectively and their wild-type strains, and confirmed that succinic acid and aspartic acid were similarly affected in the modified strains, but proline content was altered contrarily. Additionally, the potential mechanism was postulated accordingly.…”

GC‐MS Based Metabolomics for Rapid Simultaneous Detection of Escherichia coli O157:H7, Salmonella Typhimurium, Salmonella Muenchen, and Salmonella Hartford in Ground Beef and Chicken

“…Maharjan and Ferenci (2005) were able to differentiate E. coli strains from different origins by 2‐dimensional high‐performance thin layer chromatography. Modified strains of E. coli were classified from their wild type counterparts by GC‐FID and GC‐MS with succinic acid, proline, and asparctic acid as the main metabolites responsible for the classification (Tian and others 2008). Metabolite‐based efforts for rapid detection of E. coli and Salmonella have been carried out using an electronic nose (Siripatrawan and others 2006) and neutral desorption sampling coupled to extractive electrospray‐ionization MS (Chen and others 2007); however, no specific biomarkers for each target pathogen were reported.…”

Metabolic profiling of transgenic rice with cryIAc and sck genes: An evaluation of unintended effects at metabolic level by using GC-FID and GC–MS

“…Compared to GC-MS in full scan monitoring mode, GC-FID is considered of higher sensitivity, more reliable repetition and wider dynamic range, thus, can be better adopted in quantitative analysis. Combining GC-MS and GC-FID to utilize both advantages is more preferable and has been applied to many types of samples [27,28].…”