Phenotype MicroArrays as a complementary tool to next generation sequencing for characterization of tree endophytes

Blumenstein, Kathrin; Macaya‐Sanz, David; Martin, Juana A.; Albrectsen, Benedicte Riber; Witzell, Johanna

doi:10.3389/fmicb.2015.01033

Cited by 34 publications

(20 citation statements)

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“…But the long-term effect of the endophytic fungi against H. fraxineus could not be observed [142]. In several studies, in vitro antagonism assays revealed antagonistic effects of endophytic fungi towards pathogens [20,66,252,267,274]. Nevertheless, the stable establishment and survival of the endophyte in planta is not necessarily guaranteed.…”

Section: Prospects For Translational Applications Of the Knowledge Fomentioning

confidence: 99%

Forest Tree Microbiomes and Associated Fungal Endophytes: Functional Roles and Impact on Forest Health

Terhonen

Blumenstein

Kovalchuk

et al. 2019

Forests

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173

113

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Terrestrial plants including forest trees are generally known to live in close association with microbial organisms. The inherent features of this close association can be commensalism, parasitism or mutualism. The term “microbiota” has been used to describe this ecological community of plant-associated pathogenic, mutualistic, endophytic and commensal microorganisms. Many of these microbiota inhabiting forest trees could have a potential impact on the health of, and disease progression in, forest biomes. Comparatively, studies on forest tree microbiomes and their roles in mutualism and disease lag far behind parallel work on crop and human microbiome projects. Very recently, our understanding of plant and tree microbiomes has been enriched due to novel technological advances using metabarcoding, metagenomics, metatranscriptomics and metaproteomics approaches. In addition, the availability of massive DNA databases (e.g., NCBI (USA), EMBL (Europe), DDBJ (Japan), UNITE (Estonia)) as well as powerful computational and bioinformatics tools has helped to facilitate data mining by researchers across diverse disciplines. Available data demonstrate that plant phyllosphere bacterial communities are dominated by members of only a few phyla (Proteobacteria, Actinobacteria, Bacteroidetes). In bulk forest soil, the dominant fungal group is Basidiomycota, whereas Ascomycota is the most prevalent group within plant tissues. The current challenge, however, is how to harness and link the acquired knowledge on microbiomes for translational forest management. Among tree-associated microorganisms, endophytic fungal biota are attracting a lot of attention for their beneficial health- and growth-promoting effects, and were preferentially discussed in this review.

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Section: Prospects For Translational Applications Of the Knowledge Fomentioning

confidence: 99%

Forest Tree Microbiomes and Associated Fungal Endophytes: Functional Roles and Impact on Forest Health

Terhonen

Blumenstein

Kovalchuk

et al. 2019

Forests

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173

113

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“…In the recent past years, asymptomatic endophytic fungi have been also regarded as potential biocontrol agents for tree diseases (e.g., Arnold et al 2003;Blumenstein et al 2015). The oak decline has been correlated with the diversity and amount of fungal endophytes present on different tissues of Quercus spp., and many oak-specific endophytes are specifically described to accelerate the decline of oaks stand (Ragazzi et al 2001(Ragazzi et al , 2003(Ragazzi et al , 2004.…”

Section: Disturbance and Protection Of Fagaceae Forests From Biotic Tmentioning

confidence: 99%

Mycorrhization of Fagaceae Forests Within Mediterranean Ecosystems

Reis

Tavares

Baptista

et al. 2017

Mycorrhiza - Function, Diversity, State of the Art

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Mediterranean Fagaceae forests are valuable due to their ecological and socioeconomic aspects. Some profitable plant species, such as Castanea (timber and chestnut), Quercus (timber and cork), and Fagus (timber), encounter in this habitat the excellent edaphoclimatic conditions to develop. All Fagaceae plants are commonly associated to ECM fungal species, which are found in these forests in quite stable communities, mainly enriched in Russulaceae and Telephoraceae species. Currently, the Mediterranean Basin is considered as one of the global biodiversity hotspots, since many of their endemic plant species are not found elsewhere and are now under threat. Due to climate changing and introduction of disease agents, Fagaceae forests are facing an adaptation challenge to both biotic and abiotic threats. Although ECM communities are highly disturbed by climate factors and tree disease incidence, they could play an important role in increasing water availability to the plant and also improving plant tree defense against pathogens. Recent advances, namely, on genomics and transcriptomics, are providing tools for increasing the understanding of Fagaceae mycorrhization process and stress responses to biotic and abiotic stresses. Such studies can provide new information for the implementation of the most adequate management policies for protecting threaten Mediterranean forests.

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“…its endophytes, as well as between the endophytes and pests and pathogens dependant on that tissues. The details of these possible interactions are not known [20,24,54]. We envisage a few possible scenarios of these interactions here with a view to design better methodologies to improve the performance of endophytes as biocontrol agents.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, inoculating a crop with an endophyte which upregulates most defence-related genes or elaborates insect-deterring metabolites could be an effective strategy. Alternatively, an endophyte could exclude a pathogen if its nutritional niche overlaps that of the pathogen [54] (Fig. 5a).…”

Section: Discussionmentioning

confidence: 99%

Biological Control Through Fungal Endophytes: Gaps In Knowledge Hindering Success

Suryanarayanan¹,

Rajulu

Vidal

2018

CBIOT

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Background: The asymptomatic fungal endosymbionts of plants called the endophytes are universal in their occurrence. The occurrence of some entomopathogenic fungi which infect and kill insect pests of crops as endophytes provides an excellent opportunity for using these fungi in biocontrol. A few studies substantiate this novel approach of pest management; however, since this facet of endophyte research is nascent, enquiries answering basic questions on various aspects of endophyte biology are essential to successfully implement endophytemediated biocontrol. In a collaborative research, we addressed a few of these questions including (i) to what extent do fungal genera reported to have entomopathogenic species colonize non-crops as endophytes? (ii) can an endophyte from a non-crop source and vested with anti-insect property survive as an endophyte when introduced into a crop? (iii) how does an anti-insect endophyte in a crop affect an insect pest? and (iv) can endophytes be used for controlling weeds? The results of this study show that endophytes have enormous potential as biocontrol agents and reiterate the need for concerted efforts to obtain more basic information about endophyte biology before they can be used effectively for biocontrol. Methods: Non-entomopathogenic but anti-insect metabolites producing endophytes from plant and nonplant sources are shown to have potential biocontrol properties. Survival of an effective fungus from an alien source in a crop plant as an endophyte has been shown to be only for a limited period. Endophytes are shown to possess weedicidal activity. Results: Entomopathogenic fungal genera rarely colonize leaves of forest trees as foliar endophytes. Non-entomogenous endophytes produce ant-insect compounds affecting pests of crops thus having the potential as biocontrol agents. Such fungi isolated from non-plant sources can be inoculated into crops to function as endophytes, for brief periods. Insect pests avoid crops with anti-insect endophytes for oviposition and feeding. Some endophytes elaborate weedicidal compounds. Conclusion: Our basic study underscores the need to screen plants and non-plants of different habitats for entomopathogenic and anti-insect endosymbiotic fungi. Futhermore, it highlights the importance of screening endophytes for weedicidal compounds. We emphasise the need for focused studies for better understanding of the interaction between endophytes and their plant hosts and the biotic stressors of the host plants to enhance the success of using endophytes for biological control.

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Phenotype MicroArrays as a complementary tool to next generation sequencing for characterization of tree endophytes

Cited by 34 publications

References 50 publications

Forest Tree Microbiomes and Associated Fungal Endophytes: Functional Roles and Impact on Forest Health

Forest Tree Microbiomes and Associated Fungal Endophytes: Functional Roles and Impact on Forest Health

Mycorrhization of Fagaceae Forests Within Mediterranean Ecosystems

Biological Control Through Fungal Endophytes: Gaps In Knowledge Hindering Success

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