2014
DOI: 10.1038/cmi.2014.8
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Phenotypes of Th lineages generated by the commonly used activation with anti-CD3/CD28 antibodies differ from those generated by the physiological activation with the specific antigen

Abstract: T-helper (Th) lineages have been generated in vitro by activating CD4 cells with anti-CD3/CD28 antibodies during polarization. Physiologically, however, the generation of Th lineages is by activation with the specific antigen presented by antigen-presenting cells (APC). Here, we used TCR-transgenic mice to compare the phenotypes of Th1, Th9 and Th17 lineages when generated by either one of the two activation modes. Lineage Th cells specific against hen egg lysozyme (HEL), were adoptively transferred into recip… Show more

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Cited by 16 publications
(10 citation statements)
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References 23 publications
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“…Activation by anti-CD3/CD28 antibodies versus cognate antigen recognition differentially regulates gene transcription in CD4 T cells (14) and results in differential TCR signaling kinetics also in γδ T cells (25). This study adds a further facet to the pattern of Vδ2 T-cell gene expression, which has been described as being intermediate between αβ T cells and natural killer cells (26).…”
Section: Discussionmentioning
confidence: 78%
See 1 more Smart Citation
“…Activation by anti-CD3/CD28 antibodies versus cognate antigen recognition differentially regulates gene transcription in CD4 T cells (14) and results in differential TCR signaling kinetics also in γδ T cells (25). This study adds a further facet to the pattern of Vδ2 T-cell gene expression, which has been described as being intermediate between αβ T cells and natural killer cells (26).…”
Section: Discussionmentioning
confidence: 78%
“…In general, IL-4, together with TGF-β, was found to polarize IL-9-secreting CD4 T cells (10)(11)(12), and PU.1 and interferon regulatory factor 4 (IRF4) were identified as crucially important transcription factors (11,13). The signal strength of T-cell receptor (TCR) ligation and costimulatory signals also regulate IL-9 at the transcriptional level (14).…”
mentioning
confidence: 99%
“…(16) and (19) for detail. All mice were kept under specific pathogen-free conditions in the National Eye Institute’s animal facility and all experimental procedures were performed in compliance with the NIH Resolution on the Use of Animals in Research, under protocol NEI-555.…”
Section: Methodsmentioning
confidence: 99%
“…Thus, TCR-derived signaling is indispensable for induction of Th9 cells. IL-9 gene expression is positively correlated with the strength of TCR signals [49, 54]. TCR stimulation activates the transcription factor NFAT1 and NF-κB (p65), promoting their nuclear localization to stimulate transcription of IL-9 [52].…”
Section: T Cell Receptor Signalingmentioning
confidence: 99%