Phenotypic Analysis of Hepatic Lymphocytes from Healthy Dogs

Abstract

ABSTRACT. Phenotypes of lymphocytes from laparoscopically biopsied liver tissues of eleven healthy beagle dogs were analyzed. CD21-lymphocytes (non-T non-B lymphocytes), and the CD4 + /CD8 + ratio in the canine hepatic lymphocytes were 54.8 ± 11.9%, 4.7 ± 3.1%, 40.7 ± 13.2%, and 0.33 ± 0.12, respectively, while those in peripheral blood lymphocytes were 85.4 ± 6.5%, 9.3 ± 6.1%, 5.3 ± 1.8%, and 1.64 ± 0.36, respectively. These results indicated that the constitution of hepatic lymphocytes quite differed from that of peripheral blood lymphocytes in dogs, and suggested that the regional immunity in canine liver might be specific.

Flow cytometric evaluation of peripheral blood and bone marrow and fine-needle aspirate samples from multiple sites in dogs with multicentric lymphoma

“…of different sample collection methods. 24,25 For example, investigators in 1 study 24 reported a mean ratio of CD3-positive-to-CD21-positive cells of 2.1 in spleen samples from 3 healthy Beagles, whereas the mean T:B identified in spleen samples of 5 healthy Beagles in the present study was 2.3.…”

“…Some flow cytometry-based studies have focused on lymphocyte development in lymphatic organs (including the spleen) 24 or the characterization of lymphocytes found in the liver in healthy dogs. 25 In an earlier study, 29 investigators analyzed T-cell subsets in lymph nodes, spleen, bone marrow, thymus, and blood of healthy dogs by use of monoclonal antibodies. Another flow cytometry-based study 26…”

“…22,23 To the authors' knowledge, comparable studies to evaluate lymphocytes obtained in fine-needle aspirate samples from the liver or spleen of lymphomaaffected dogs have not been reported. Investigators of previous studies [24][25][26] used flow cytometry to evaluate tissue samples obtained from the liver or spleen of dogs via biopsy or necropsy. The objective of the study reported here was to determine whether the extent of disease in dogs with multicentric lymphoma can be assessed via flow cytometry.…”

Phenotypic Analysis of Hepatic T Lymphocytes in a Dog with Chronic Hepatitis

“…In our dog, there were large numbers of CD3+ lymphocytes within the liver tissue. CD8+ lymphocytes are found to be more numerous than CD4+ lymphocytes in the liver of healthy dogs [4]. However, hepatic CD4+ lymphocytes were predominant in this case.…”

“…Cryostat sections (8 µm in thick) were prepared and stained by the avidin-biotin-peroxidase complex method with an antibody against human CD3 (CD3-12; Serotec, Oxford, UK). Furthermore, hepatic and peripheral lymphocytes were analyzed using anti-canine CD3 fluorescein isocyanate conjugated (-FITC) antibody (CA17.2A12; Serotec), antihuman CD21 phycoerythrin conjugated (-PE) antibody (Bly4; Becton-Dickinson, Franklin Lakes, NJ), anti-canine CD4-FITC antibody (YKIX302.9; Serotec), and anti-canine CD8α-PE antibody (YCATE55.9; Serotec) by flow cytometric method as described by Sakai et al [4]. Phenotypes were identified on the basis of the lymphocytes of 5,000 events obtained by gating on the screen for the forward scatter versus side scatter dot plot of a FACS Calibur (BectonDickinson).…”