Phenotypic and cytological variation among plants derived from anther cultures ofLilium longiflorum

Qu, Yanghua; Mok, Machteld C.; Mok, David W. S.; Stang, J. R.

doi:10.1007/bf02628501

Cited by 23 publications

(12 citation statements)

References 3 publications

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“…The present study showed that picloram is suitable for inducing yellowish nodular calluses in anther cultures of the cultivar 'Connecticut King', whereas 2,4-D had no effect on nodular callus production in anthers from both field-and greenhouse-grown plants, although it has been used for producing haploids in anther cultures of other Lilium species (Gu and Cheng, 1982;Qu et al 1988). It has already been reported that picloram gave better results than 2,4-D for both callus induction and subsequent morphogenesis in several Liliaceous species: somatic embryogenesis from leaf sections of Gasteria and Haworthia (Beyl and Sharma, 1983), and callus formation and plant regeneration from shoot tips of Allium cepa (Phillips and Luteyn, 1983).…”

Section: Discussionmentioning

confidence: 87%

“…Establishment of homozygous lines through in vitro culture could also provide additional means for improvement of lilies with respect to floral and marketable qualities. Haploid or doubled haploid plants of lilies have already been produced in vitro: haploids of L. longiflorum were obtained by anther culture but haploidy was lost after four to six subcultures of them (Sharp et al, 1971), haploid, diploid and aneuploid plantlets were regenerated from anther cultures of L. davidii (Gu and Cheng, 1982), gynogenic doubled haploid Plantlets were regenerated from unfertilized ovules of the Oriental hybrid lily 'Leraza' (Prakash and Giles, 1986); and diploid and mixoploid (with up to 80% haploid cells) plants were regenerated from anther cultures of several L. longiflorum genotypes (Qu et al, 1988). However, an efficient and reproducible system for producing a large number of haploid lily plants has not yet been developed, and production of haploid plants has been restricted to only a few species and cultivars described above.…”

Section: Introductionmentioning

confidence: 99%

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Regeneration of haploid plants from anther cultures of the Asiatic hybrid lily ‘Connecticut King’

Han

Niimi

Nakano

1997

Plant Cell Tiss Organ Cult

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A system for producing haploid plants from anther cultures was developed for the Asiatic hybrid lily 'Connecticut King'. Anthers containing microspores at the mid-to late-uninucleate stages were cultured on MS media supplemented with various plant growth regulators. Microspores containing 3 or 4 vegetative-like nuclei were observed 2 to 3 weeks later, and yellowish nodular calluses appeared within dehisced anthers 2 to 3 months after culture. Picloi'am was superior to 2,4-D for inducing nodular calluses. Anthers from greenhouse-grown plants required higher concentrations of both picloram and cytokinins than those from field-grown plahts and most frequently produced nodular calluses (17.6%) on MS medium containing 2 mg 1-1 picloram and 2 mg 1-1 zeatin. The nodular calluses regenerated many bulblets following transfer to MS medium supplemented with 0,1 or 0.5 mg I-l picloram and 0.01 mg 1-1 BA, and the bulblets developed into plantlets (bulblets with scaly leaves and roots) after transfer to MS medium containing 0.1 mg 1-1 NAA. Chromosome counts of root-tip cells of 11 plantlets revealed that five were haploids (2n = 12), two diploids (2n = 24), and four mixoploid. This result suggests that at least some plantlets were of gametophytic origin.

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Section: Discussionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Regeneration of haploid plants from anther cultures of the Asiatic hybrid lily ‘Connecticut King’

Han

Niimi

Nakano

1997

Plant Cell Tiss Organ Cult

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“…The morphological variation of regenerants was recorded in the height of regenerants, leaf size, shape, and length:width ratio, and fruit size, among other factors (Qu et al 1988;Kiss et al 2001;Kozik et al 2002;Rimberia et al 2006). Cytological differences recorded in in vitro regenerants included aneuploidy, haploidy, diploidy, triploidy, tetraploidy, octoploidy, nanoploidy, dodecaploidy, and mixoploidy (Ockendon 1998;Kim et al 2003;Rimberia et al 2006).…”

Section: Discussionmentioning

confidence: 99%

“…In ornamental plants, variation among plants derived from anther cultures was recorded in Lilium longiflorum (Qu et al 1988;Arzate-Fernández et al 1997), Asiatic hybrid lily (Han et al 1997), Helianthus annuus L. 'Morden' (Thengane et al 1994;Saji and Sujatha 1998), Dianthus chinensis L. (Fu et al 2008), and Gentiana triflora (Doi et al 2010). In other horticultural plants, variation was reported in Daucus carota (Kozik et al 2002), Citrus clementina Hort.…”

Section: Introductionmentioning

confidence: 92%

“…Aneuploidy, haploidy, diploidy, triploidy, tetraploidy, octoploidy, mixoploidy, etc. were cytological alterations commonly derived from anther culture (Qu et al 1988;Thengane et al 1994;Arzate-Fernández et al 1997;Han et al 1997;Ockendon 1998;Saji and Sujatha 1998;Chiancone et al 2006;Rimberia et al 2006;Kowalska et al 2008;Doi et al 2010).…”

Section: Introductionmentioning

confidence: 98%

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Morphological and cytological diversity of regenerants derived from half-anther cultures of anthurium

Winarto¹,

Rachmawati²,

Pramanik³

et al. 2010

Plant Cell Tiss Organ Cult

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Anthurium anther culture was successfully established using half-anthers as explants. Explants were cultured on Winarto-Teixeira basal medium (WT-1) containing 0.01 mg/l a-naphthalene acetic acid (NAA), 0.5 mg/l thidiazuron (TDZ), and 1.0 mg/l 6-benzylaminopurine (BAP), or on New Winarto-Teixeira basal medium (NWT-3) supplemented with 0.02 mg/l NAA, 1.5 mg/l TDZ, and 0.75 mg/l BAP for callus initiation. Regenerated calli produced multiple shoots on WT-1, which were then rooted in NWT-3 supplemented with 1% activated charcoal. Plantlets were acclimatized ex vitro using a mixture of burned rice husk, rice husk, and bamboo peat (1:1:1, v/v/v) as the potting medium. There was considerable morphological and cytological diversity of regenerants derived from anther culture, which are described in detail in this study. The callus cluster color ranged from green to light green and had a high regeneration capacity (7.3 and 4.8 shoots/callus cluster), light reddish-yellow callus showed moderate regeneration (2.6 shoots/callus cluster), while reddish-yellow callus had the lowest regeneration capacity (1.5 shoots/callus cluster). Morphological variations clearly observed in regenerants derived from this technique included alterations in plant size, peduncle length, spathe position compared to leaves, the type and number of buds, spathe and spadix color, and spadix length. There were also cytological variations in both in vitro and ex vitro regenerants of anther culture with 23-29% haploids, 5-10% aneuploids, 56-69% diploids, and 3-4% triploids. The results strengthen other studies in which the development of anther cultures, especially via callus formation, resulted in morphological and cytological alterations. These variations have been discussed to great length in this paper.

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Tissure Culture of Ornamental Flowering Bulbs (Geophytes)

KiuWeon

Hertogh

1996

Horticultural Reviews

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Phenotypic and cytological variation among plants derived from anther cultures ofLilium longiflorum

Cited by 23 publications

References 3 publications

Regeneration of haploid plants from anther cultures of the Asiatic hybrid lily ‘Connecticut King’

Regeneration of haploid plants from anther cultures of the Asiatic hybrid lily ‘Connecticut King’

Morphological and cytological diversity of regenerants derived from half-anther cultures of anthurium

Tissure Culture of Ornamental Flowering Bulbs (Geophytes)

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