Phenotypic and functional characteristics of porcine peritoneal mesothelial cells

Ohan, Jeanny; Gilbert, M. A.; Brouland, Jean‐Philippe; Rougier, Jean-Philippe; Trugnan, Germain; Wassef, Michel; Lesèche, Guy; Drouet, Ludovic

doi:10.1007/s11626-999-0102-9

Cited by 5 publications

(1 citation statement)

References 54 publications

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“…Addition of plateletderived growth factor or EGF to the culture medium stimulated porcine mezothelial cells (PMCs). PMCs constitutively expressed fibrinolytic and procoagulant activity [9].…”

Section: Introductionmentioning

confidence: 99%

Reduction of peritoneal adhesions by sustained and local administration of epidermal growth factor

et al. 2007

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Previous studies have shown epidermal growth factor (EGF) facilitate peritoneal membrane healing by augmenting cell adhesion and migration. The objective of this study was to show the effect of sustained and local administration of EGF on peritoneal adhesion. Fourty-two rats were divided into six groups: control 7 and 14, gelatin 7 and 14, and EGF 7 and 14. Adhesions were created by scraping the cecum with mesh gause followed by application of absolute alcohol and placement of silk suture in the parietal peritoneum. The anterior walls of the intestines were covered with 5 x 5 cm unloaded, and EGF loaded gelatin films in the gelatin and EGF groups, respectively. The rats were killed on days 7 and 14 to assess the adhesion occurring, and for biochemical examination. The mean adhesion grades of EGF groups were significantly lower than in the other groups (P < 0.008). The mean adenosine deaminase (ADA) measurements of EGF 7 group were lower than in the gelatin 7 and control 7 groups but the difference was not significant (P > 0.008). The mean ADA measurements in the 14 days groups were as follows: control 14 < EGF 14 < gelatin 14 groups. The mean ADA measurements between 14 days groups did not significantly differ from each other (P > 0.008). The mean hydroxyproline measurements did not differ among the groups (P > 0.008). EGF decreased intestinal adhesion in our study. EGF has important roles in DNA synthesis and cell proliferation. Further studies are required to determine the exact mechanism by which EGF lowers the efficiency of intestinal adhesion.

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Section: Introductionmentioning

confidence: 99%

Reduction of peritoneal adhesions by sustained and local administration of epidermal growth factor

et al. 2007

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N-acetyl-l-cysteine decreases intra-abdominal adhesion formation through the upregulation of peritoneal fibrinolytic activity and antioxidant defenses

Chu

Lim

Heydrick

et al. 2011

Surgery

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Human mesothelial cells express tissue factor when switched to proliferating state. Pharmacological modulation in vitro

Ohan

Lesèche²,

Ma³

et al. 2001

Blood Coagulation and Fibrinolysis

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Covering the inner surface of small-diameter arterial prostheses with endothelial cells (ECs) has been proposed as a means of improving biocompatibility and thrombosis resistance. Because the availability of autologous ECs is limited, autologous human mesothelial cells (HMCs) have been suggested as a substitute for ECs. However, HMCs express tissue factor (TF) in vitro, a deleterious characteristic in vivo. We investigated the distribution of TF antigen and of its inhibitor, tissue factor pathway inhibitor, on HMCs and the effect of pharmacological agents on TF expression. TF antigen was measured by enzyme-linked immunosorbent assay and localized by confocal microscopy. Three distinct pools of TF antigen were demonstrated: within the cells, at the cell surface, and in the extracellular matrix. The effects of ilomedin (10 microg/ml) and heparin (500 U/ml), known to affect procoagulant activity, were evaluated by incubating HMCs for 24 h with or without these agents. Ilomedin, but not heparin, decreased TF antigen expression by 30% (P < 0.05). Despite the theoretical potential of HMCs as a vascular prosthesis lining, TF expression by HMCs remains a major drawback. A technique capable of blocking TF expression until the HMCs return to their resting state is needed. Genetic manipulation of HMCs may hold promise for such a technique.

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Phenotypic and functional characteristics of porcine peritoneal mesothelial cells

Cited by 5 publications

References 54 publications

Reduction of peritoneal adhesions by sustained and local administration of epidermal growth factor

Reduction of peritoneal adhesions by sustained and local administration of epidermal growth factor

N-acetyl-l-cysteine decreases intra-abdominal adhesion formation through the upregulation of peritoneal fibrinolytic activity and antioxidant defenses

Human mesothelial cells express tissue factor when switched to proliferating state. Pharmacological modulation in vitro

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