Phenotypic and Functional Differences Between Rat Alveolar, Pleural, and Peritoneal Macrophages

Dörger, Martina; Münzing, Silvia; Allmeling, Anne-Marie; Meßmer, K.; Krombach, Fritz

doi:10.1080/019021401459770

Cited by 30 publications

(19 citation statements)

References 22 publications

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“…The differential expression of cytokines between alveolar and peritoneal macrophages might be explained by different biological responses of two different cell populations (13,48). Salez et al (48) demonstrated that differentiation of mononuclear cells depends on the tissue environment: for example, the pulmonary environment allows alveolar macrophages to have different functions from peritoneal macrophages even though they derive from the same population of circulating monocytes.…”

Section: Discussionmentioning

confidence: 99%

Recombinant Guinea Pig Tumor Necrosis Factor Alpha Stimulates the Expression of Interleukin-12 and the Inhibition ofMycobacterium tuberculosisGrowth in Macrophages

et al. 2005

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Section: Discussionmentioning

confidence: 99%

Recombinant Guinea Pig Tumor Necrosis Factor Alpha Stimulates the Expression of Interleukin-12 and the Inhibition ofMycobacterium tuberculosisGrowth in Macrophages

et al. 2005

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“…Their functional and phenotypic characteristics depend on their location and are determined by the surrounding micromilieu. Several animal experiments have compared the phenotype and inflammatory capacity of pleural mu to peritoneal and alveolar mu [6,7]. Moreover, in a recent study, using selective mu depletion in mice, their functional importance for the initiation of an inflammatory reaction in the pleural cavity has been shown [8].…”

Section: Introductionmentioning

confidence: 98%

Pleural macrophages are the dominant cell population in the thoracic cavity with an inflammatory cytokine profile similar to peritoneal macrophages

et al. 2007

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Numerous human macrophage (mphi) subpopulations with different behavior have been identified in adults. It is well known that peritoneal mphi are activated by abdominal surgery and subsequently contribute to a systemic inflammatory response that leads to immune suppression, increased morbidity and mortality. Information on the role of pleural mphi in adults is scarce and information on their role in children is lacking. We investigated the behavior of pleural versus peritoneal mphi in children and adolescents. As a first step, we compared the cellular composition of the pleural and peritoneal surface in children and adolescents. Pleural and peritoneal lavages were performed in 21 patients undergoing non-contaminated laparoscopic and thoracoscopic surgical procedures. We observed a significantly higher percentage of mphi in the pleural compared to the peritoneal cavity with less lymphocytes, a small amount of polymorphonuclear cells (PMNs) and other cells. To further study the mphi inflammatory response, we measured the spontaneous and LPS triggered cytokine release of isolated pleural versus peritoneal mphi (IL-1beta, IL-6, and IL-10). The pattern of cytokine release was similar in both, pleural and peritoneal mphi. Directly after lavage, they showed a strong activation, with no difference between stimulated and non-stimulated cells. After 24 h resting, mphi of both compartments reacted to LPS with a similar significant increase in the cytokine release. In conclusion, our results demonstrate that pleural mphi represent the dominant cell population in the pleural cavity of the young. They show a similar inflammatory response as peritoneal mphi and should be considered to play a major role in the local inflammatory response to thoracic surgery.

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“…Findings of previous studies have relied upon nonalveolar and nonhuman macrophages, and the significance of some findings is obscured by different responses of alveolar macrophages compared with blood macrophages. For example, studies have shown alveolar macrophages to be selectively up-regulated by cytokines and LPS compared with same-donor blood monocytes (10,11). However, the role of antigens of respiratory bacteria on this process has not been explored.…”

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confidence: 99%

Impaired Alveolar Macrophage Response to Haemophilus Antigens in Chronic Obstructive Lung Disease

Berenson

Wrona

Grove

et al. 2006

Am J Respir Crit Care Med

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Rationale: Interactions of nontypeable Haemophilus influenzae (NTHI) with macrophages are implicated in the pathogenesis of chronic obstructive pulmonary disease (COPD). However, the immunologic mechanisms that mediate NTHI-macrophage inflammation are poorly understood. Outer membrane protein (OMP) P6 and lipooligosaccharide (LOS) of NTHI are potent immunomodulators. We theorized that alveolar macrophages in COPD possess fundamental immune defects that permit NTHI to evade host responses. Objective: To test this hypothesis, we obtained human alveolar and blood macrophages from exsmokers with COPD, exsmokers without COPD, and nonsmokers. Methods: Alveolar and blood macrophages from each donor were incubated with purified LOS and OMP P6 and with OMP P2 and the total outer membrane preparation (0.1-1 g/ml). Measurements: Supernatants (24 h) were assayed for IL-1␤, TNF-␣, IL-10, IL-12, and IL-8 by multianalyte multiplexed flow cytometry. Results: Comparative induction of COPD and non-COPD alveolar macrophages by LOS and OMP P6 revealed diminished IL-8, TNF-␣, and IL-1␤ responses of COPD alveolar macrophages (p р 0.03 for each). COPD alveolar macrophages also had diminished responses to total outer membrane (p р 0.03 for each). In contrast, COPD blood macrophages had no significant differences among donor groups in IL-8, TNF-␣, or IL-1␤ responsiveness to NTHI antigens. Diminished IL-12 responses of COPD blood macrophages to NTHI antigens, compared with nonsmokers, could not be independently dissociated from group differences in age and pack-years. Conclusions: These findings support a paradigm of defective immune responsiveness of alveolar macrophages, but not blood macrophages, in COPD.

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Phenotypic and Functional Differences Between Rat Alveolar, Pleural, and Peritoneal Macrophages

Cited by 30 publications

References 22 publications

Recombinant Guinea Pig Tumor Necrosis Factor Alpha Stimulates the Expression of Interleukin-12 and the Inhibition ofMycobacterium tuberculosisGrowth in Macrophages

Recombinant Guinea Pig Tumor Necrosis Factor Alpha Stimulates the Expression of Interleukin-12 and the Inhibition ofMycobacterium tuberculosisGrowth in Macrophages

Pleural macrophages are the dominant cell population in the thoracic cavity with an inflammatory cytokine profile similar to peritoneal macrophages

Impaired Alveolar Macrophage Response to Haemophilus Antigens in Chronic Obstructive Lung Disease

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