Phenotypic and genetic analyses of 111 clinical and environmental O1, O139, and non-O1/O139<i>Vibrio cholerae</i>strains from different geographical areas

Niemcewicz, Marcin; Bassy, Olga; Lorenzo, Paloma; Marti, Luc; Roszkowiak, Anna; Kocik, Janusz; Cabria, Juan Carlos

doi:10.1017/s0950268811002147

Cited by 7 publications

(7 citation statements)

References 36 publications

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“…Both (D13 and D15) the strains showed a multiple antibiotic resistance phenotype with resistance to streptomycin, co-trimoxazole, nalidixic acid, ciprofloxacin, ceftaxime and ofloxacin compared to some recent studies where non-O1/non-O139 were susceptible to antibiotics [23,54]. However, enhanced level of resistance to ampicillin, chloramphenicol, fluoroquinolones, cotrimoxazole have been reported by many studies [55][56][57] like the current study. However, the resistance pattern of these non-O1/non-O139 isolates was different from O1 isolates of clinical origin in Pakistan [58].…”

Section: Accepted Manuscriptsupporting

confidence: 58%

Type III secretion system confers enhanced virulence in clinical non-O1/non-O139 Vibrio cholerae

Zeb

Shah

Yasir

et al. 2019

Microbial Pathogenesis

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Vibrio cholerae O1 infections mainly are responsible for significant mortality and morbidity amongst children, however, non-O1/non-O139 V. cholerae have also been reported to cause mild to severe infections because of their virulence potential. The pathogenic mechanisms of non-O1, non-O139 isolates are not as clearly understood as for that of O1 and O139 isolates. Type three secretion system (TTSS) is also considered one of the important virulent factors and during the current study, we investigated the role of TTSS in association with non-O1/non-O139 clinical isolates. We report that the presence of TTSS in non-O1/non-O139 V. cholerae clinical isolate (D13) from a child confers more virulence compared to the one lacking it (D15) in another clinical case during the small cholera epidemic. Moreover, the antibiotic susceptibility profiles of D13 and D15 indicate that they are multiple drug resistance (MDR) isolates. The sequence analysis for TTSS cluster was carried out for D13 and compared with the TTSS positive

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Section: Accepted Manuscriptsupporting

confidence: 58%

Type III secretion system confers enhanced virulence in clinical non-O1/non-O139 Vibrio cholerae

Zeb

Shah

Yasir

et al. 2019

Microbial Pathogenesis

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“…Given that the environment is dominated by non-O1/non-O139 strains, this observation suggests that these virulence factors probably do not promote fitness in the aquatic niche although there is some data that challenges this conclusion for at least chitin binding lectins (Kirn et al 2005). Various typing methods and deep sequencing has enabled more sensitive surveillance of CTX–ϕ in strains isolated from the environment (Rivera et al 2001; Singh et al 2001; Pang et al 2007; Awasthi et al 2012; Hasan et al 2012; Sealfon et al 2012; Sellek et al 2012). The prevalent assumption that the presence of toxigenic strains in water provides proof that toxigenic strain occupy a stable environmental niche needs to be readdressed with modern methods that might differentiate between contamination of the environment by nearby cholera victims as opposed to these strains maintaining themselves within the environment in the absence of infected humans.…”

Section: Conventional Genomics and Established Virulence Islandsmentioning

confidence: 99%

Genomic Science in Understanding Cholera Outbreaks and Evolution of Vibrio cholerae as a Human Pathogen

Robins

Mekalanos

2014

Cholera Outbreaks

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Modern genomic and bioinformatic approaches have been applied to interrogate the V. cholerae genome, the role of genomic elements in cholera disease, and the origin, relatedness, and dissemination of epidemic strains. A universal attribute of choleragenic strains includes a repertoire of pathogenicity islands and virulence genes, namely the CTX–ϕ prophage and Toxin Co-regulated Pilus (TCP) in addition to other virulent genetic elements including those referred to as Seventh Pandemic Islands. During the last decade, the advent of Next Generation Sequencing (NGS) has provided highly resolved and often complete genomic sequences of epidemic isolates in addition to both clinical and environmental strains isolated from geographically unconnected regions. Genomic comparisons of these strains, as was completed during and following the Haitian outbreak in 2010, reveals that most epidemic strains appear closely related, regardless of region of origin. Non-O1 clinical or environmental strains may also possess some virulence islands, but phylogenic analysis of the core genome suggests they are more diverse and distantly related than those isolated during epidemics. Like Haiti, genomic studies that examine both the Vibrio core- and pan-genome in addition to Single Nucleotide Polymorphisms (SNPs) conclude that a number of epidemics are caused by strains that closely resemble those in Asia, and often appear to originate there and then spread globally. The accumulation of SNPs in the epidemic strains over time can then be applied to better understand the evolution of the V. cholerae genome as an etiological agent.

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“…Furthermore, the non-toxigenic O1 isolates in this study were also genetically distinct from the epidemic V. cholerae O1/O139 cluster (GT1), indicating that other unknown virulence factors could be present in the epidemic V. cholerae O1/O139 cluster that are absent in non-toxigenic V. cholerae O1 isolates. Previous studies have shown the presence of non-toxigenic V. cholerae O1 strains in the environment and in humans [6,18,21,27]. Serotyping is therefore not a reliable method for the identification of toxigenic and epidemic V. cholerae O1/O139 strains.…”

Section: Discussionmentioning

confidence: 99%

“…In total, 48 clinical and environmental isolates of V. cholerae and Vibrio mimicus (Table 1) were obtained from Instituto Tecnológico La Marañosa, Spanish Ministry of Defence, San Martín de la Vega, Madrid, Spain, Norwegian Defence Research Establishment, Kjeller, Norway, and Military Institute of Hygiene and Epidemiology, Pulawy, Poland (Table 1) [18-20]. The human isolates were all collected as part of standard patient care.…”

Section: Methodsmentioning

confidence: 99%

OmpU as a biomarker for rapid discrimination between toxigenic and epidemic Vibrio cholerae O1/O139 and non-epidemic Vibrio cholerae in a modified MALDI-TOF MS assay

Paauw¹,

Trip²,

Niemcewicz

et al. 2014

BMC Microbiol

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BackgroundCholera is an acute diarrheal disease caused by Vibrio cholerae. Outbreaks are caused by a genetically homogenous group of strains from serogroup O1 or O139 that are able to produce the cholera toxin. Rapid detection and identification of these epidemic strains is essential for an effective response to cholera outbreaks.ResultsThe use of ferulic acid as a matrix in a new MALDI-TOF MS assay increased the measurable mass range of existing MALDI-TOF MS protocols for bacterial identification. The assay enabled rapid discrimination between epidemic V. cholerae O1/O139 strains and other less pathogenic V. cholerae strains. OmpU, an outer membrane protein whose amino acid sequence is highly conserved among epidemic strains of V. cholerae, appeared as a discriminatory marker in the novel MALDI-TOF MS assay.ConclusionsThe extended mass range of MALDI-TOF MS measurements obtained by using ferulic acid improved the screening for biomarkers in complex protein mixtures. Differences in the mass of abundant homologous proteins due to variation in amino acid sequences can rapidly be examined in multiple samples. Here, a rapid MALDI-TOF MS assay was developed that could discriminate between epidemic O1/O139 strains and other less pathogenic V. cholerae strains based on differences in mass of the OmpU protein. It appeared that the amino acid sequence of OmpU from epidemic V. cholerae O1/O139 strains is unique and highly conserved.

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Phenotypic and genetic analyses of 111 clinical and environmental O1, O139, and non-O1/O139Vibrio choleraestrains from different geographical areas

Cited by 7 publications

References 36 publications

Type III secretion system confers enhanced virulence in clinical non-O1/non-O139 Vibrio cholerae

Type III secretion system confers enhanced virulence in clinical non-O1/non-O139 Vibrio cholerae

Genomic Science in Understanding Cholera Outbreaks and Evolution of Vibrio cholerae as a Human Pathogen

OmpU as a biomarker for rapid discrimination between toxigenic and epidemic Vibrio cholerae O1/O139 and non-epidemic Vibrio cholerae in a modified MALDI-TOF MS assay

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