Phenotypic and genetic analysis of Clock, a new circadian rhythm mutant in Drosophila melanogaster.

Dushay, Mitchell S.; Konopka, Ronald J.; Orr, Dominic; Greenacre, Mary L.; Kyriacou, Charalambos P.; Rosbash, Michael; Hall, Jeffrey C.

doi:10.1093/genetics/125.3.557

Cited by 56 publications

(2 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We have tested three circadian rhythm mutants: period KG00546 , Clock Jrk [20], and takeout c00632 . No effect of these mutations on the ability of males to modify their locomotor activity under the in uence of social experience was found (Table 1, Supplementary le 2).…”

Section: Modi Cation Of Locomotor Activity Level In Various Mutants A...mentioning

confidence: 99%

Genetic control of changes in locomotor activity caused by social experience in Drosophila males

Bragina,

Goncharova,

Besedina

et al. 2023

Preprint

View full text Add to dashboard Cite

Social experience (housing in a unisexual group) modifies locomotor activity in Drosophila. In females, suppression of locomotion occurs only when flies are in aggregations (Kamyshev et al. 2002a), but males retain lowered level of locomotor activity up to 5 days after their separation from a group (Panova et al. 2013). The mechanism of how social experience (housing in a group) affects locomotor activity in Drosophila males is yet unknown. To study the genetic control and clarify the mechanisms of behavioral changes resulted from social experience, we have tested the locomotor activity modifications in various mutants including those with impaired learning/memory, circadian rhythms, some biochemical pathways and sensory systems. Results of the present study show that these changes are not based on learning/memory mechanisms. The dopaminergic system seems to play a principal role in the changes of locomotor activity caused by social experience while the octopaminergic system may modulate them. Also, the dependence of this behavioral modification upon olfactory perception was shown. This implies possible participation of pheromones that should be verified in future researches. Also, with the same aim we have performed the screening of our collection of mutants carrying random autosomal insertions of PdLtransposon. Five candidate genes playing role in behavioral modifications resulted from social experience were revealed (Dek, Hel89B, RpL41, CG11791 and NaCP60E). The results imply particularly an important role of epigenetic regulation of gene expression in modification of locomotor behavior caused by social experience.

show abstract

Section: Modi Cation Of Locomotor Activity Level In Various Mutants A...mentioning

confidence: 99%

Genetic control of changes in locomotor activity caused by social experience in Drosophila males

Bragina,

Goncharova,

Besedina

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…These findings opened a whole new avenue for behavioral genetics. Soon after, the Clock gene was discovered in both Drosophila (Dushay et al, 1990) and the mouse . In 1990…”

Section: Molecular Circadian Clocksmentioning

confidence: 99%

The role of adipose tissue circadian clocks in metabolic maintenance

Shostak¹

View full text Add to dashboard Cite

show that circadian regulation of lipolysis by the adipose clock is also important for body weight control. Indeed, Fabp4-Cre Bmal1 fl/fl mice became heavier than wild-type controls kept on standard diet and developed morbid obesity when fed with high-fat diet.In summary, we found that the adipose tissue clock is responsible for the regulation of lipid mobilization and their usage as energy source. We conclude that the adipocyte clock is an integral part of the circadian system, which normal functioning is required for metabolic homeostasis neurotransmitters glutamate (Glu) and pituitary adenylate cyclase-activating protein (PACAP) which signal to the SCN. In turn, this activates signaling cascades and the phosphorylation of CREB, which can activate the transcription of Pers leading to phase-shifts of the molecular clockwork (reviewed in (Golombek and Rosenstein, 2010)). The SCN also can acquire non-photic entrainment signals via neuropeptide Y (NPY) and gamma-aminobutyric acid (GABA) containing neurons from the thalamic intergeniculate leaflet (IGL) and serotonergic termini from raphe nuclei. This combination of photic and non-photic signals leads to a more differentiated response of the SCN to light (reviewed in (Dibner et al., 2010)). Peripheral oscillatorsUbiquitous expression patterns of clock genes indicate that peripheral tissues also contain a circadian clock (Sun et al., 1997;Tei et al., 1997). Indeed many organs show circadian expression of clock genes in vivo (Yamamoto et al., 2004). Furthermore peripheral clocks seem to rely on a similar molecular oscillator mechanism as the SCN (Yagita et al., 2001). Experiments with cell lines gave the first It is important to note that other cues can take over this SCN function. For instance, timing of food intake can uncouple peripheral clocks from SCN control. Upon restricted feeding (RF), when food is provided only during the rest period, the phase relationship between the central clock and peripheral clocks is inversed (Damiola et al., 2000;Stokkan et al., 2001). Circadian clock and metabolismDay-night variations in food consumption, activity and rest imply daily changes in the body's energy state. Indeed, accumulating evidence indicates that circadian rhythms and metabolism are tightly Table 1. Metabolic defects in mice harboring mutations in clock genes. Modified from (Sahar and Sassone-Corsi, 2012). CryptochromesOther important members of the TTL's negative loop are the Cryptochrome genes. Mice lacking both Cry genes are arrhythmic in constant darkness and are frequently used as general clock-deficient model (van der Horst et al., 1999). Not surprisingly, Cry1 -/-Cry2 -/mutants also exhibit a large variety of metabolic disturbances. Cry-deficient animals exhibit a marked reduction in size and body weight compared with controls (Bur et al., 2009). Nevertheless, Cry1 -/-Cry2 -/mice show perturbed sugar metabolism with hyperglycemia and glucose intolerance, hallmarks of diabetes (Lamia et al., 2011).Although blood triglyceride levels of Cry-deficient mice are r...

show abstract

Behavioral and molecular analyses suggest that circadian output is disrupted by disconnected mutants in D. melanogaster.

1992

View full text Add to dashboard Cite

Mutations in the disconnected (disco) gene act to disrupt neural cell patterning in the Drosophila visual system. These mutations also affect adult locomotor activity rhythms, as disco flies are arrhythmic under conditions of constant darkness (DD). To determine the state of the circadian pacemaker in disco mutants, we constructed with pers double mutants (a short period allele of the period gene) and assayed their behavioral rhythms in light‐dark cycles (LD), and their biochemical rhythms of period gene expression under both LD and DD conditions. The results demonstrate that disco flies are rhythmic, indicating that they have an active circadian pacemaker that can be entrained by light. They also suggest that disco mutants block or interfere with elements of the circadian system located between the central pacemaker and its outputs that mediate overt rhythms.

show abstract

Phenotypic and genetic analysis of Clock, a new circadian rhythm mutant in Drosophila melanogaster.

Cited by 56 publications

References 60 publications

Genetic control of changes in locomotor activity caused by social experience in Drosophila males

Genetic control of changes in locomotor activity caused by social experience in Drosophila males

The role of adipose tissue circadian clocks in metabolic maintenance

Behavioral and molecular analyses suggest that circadian output is disrupted by disconnected mutants in D. melanogaster.

Contact Info

Product

Resources

About