Phenotypic and genetic stability of new Indonesian melon cultivar (Cucumis melo L. ‘Melonia’) based on ISSR markers

Daryono, Budi Setiadi; Subiastuti, Aprilia Sufi; Fatmadanni, Arvita; Sartika, Dian

doi:10.13057/biodiv/d200419

Cited by 22 publications

(27 citation statements)

References 23 publications

(22 reference statements)

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“…This character is determined by the interaction results between proteins in the cell. Meanwhile, observations of phenotypic characters are classified into two types based on the measurement and variety continuity, namely the qualitative and quantitative phenotype character (Daryono et al, 2019). In this study, F2 was from hybrid crosses (heterozygous) with unstable phenotypic characters and there was segregation.…”

Section: Resultsmentioning

confidence: 99%

Stability of watermelon phenotype characters (Citrullus lanatus (Thunb.) Matsum. & Nakai) from crossing ♀ ‘Putri Delima’ with ♂ ‘Maduri’

Rahmandhias

Wibowo

Subiastuti

et al. 2021

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Watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai) is a horticultural plant that belongs to the Cucurbitaceae family with high public demand, however, local markets sometimes have limited supply. The existence of watermelon varieties that are not pest-resistant causes its production to be erratic. Therefore, plant breeding efforts are required to produce superior varieties through the stability test of plant characters. For watermelon to be certified as a new variety, it needs to possess a stable and adaptive character to various conditions. The F1 watermelon from crossing ♀ ‘Putri Delima’ with ♂ ‘Maduri’ produces inole-shaped fruit, red flesh, and a sweet taste. Therefore, this study aims to determine the stability of the phenotypic character of watermelon F2 from crossing ♀ ‘Maduri’ with cultivar ♂ ‘Putri Delima’ and was conducted in Jamusan, Bokoharjo, Prambanan, Sleman, D.I.Yogyakarta fields from August to December. There are five samples of ripe watermelons that were selected randomly while their phenotypic characters were observed qualitatively and quantitatively. Each F2 watermelon character was compared to F1, and the quantitative analysis was conducted using one-factor ANOVA with a confidence level of 5%. The results of quantitative character analysis between F2 and F1 showed a P (P-value)> 0.05. Meanwhile, the results of qualitative observations of F2 watermelon showed different flesh and skin color, while the harvest time from F1 was caused by the segregation of heterozygous crosses. Therefore, it is necessary to select superior phenotypic characters as desired for the next breeding.

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Section: Resultsmentioning

confidence: 99%

Stability of watermelon phenotype characters (Citrullus lanatus (Thunb.) Matsum. & Nakai) from crossing ♀ ‘Putri Delima’ with ♂ ‘Maduri’

Rahmandhias

Wibowo

Subiastuti

et al. 2021

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“…The highest similarity was found in varieties G14 and G16, with a coefficient of 0.88. The higher the similarity index indicates that these individuals have a close phenetic similarity and vice versa (Daryono et al 2019). None of the cultivars were identified as 100% similar to each other.…”

Section: Similarities Of Superior Durian Gunungpatimentioning

confidence: 97%

Inter Simple Sequence Repeat molecular markers to reveal the genetic diversity of superior durian of Gunungpati, Semarang, Indonesia

Nasrika

Retnoningsih

2021

Biodiversitas

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Abstract. Nasrika E, Retnonongsih A. 2021. Inter Simple Sequence Repeat molecular markers to reveal the genetic diversity of superior durian of Gunungpati, Semarang, Indonesia. Biodiversitas 22: 4054-4059. Kalimantan is the center of many tropical fruits, including durian. One of the edible durians and favorited by many is Durio zibethinus, which has many superior varieties. Almost all regions in Indonesia have superior durian varieties; for example, superior durian Gunungpati from Gunungpati Sub-district, the center for producing durian in Semarang City, Central Java, Indonesia. ISSR analysis was carried out to reveal the genetic diversity of the superior durian Gunungpati. DNA isolation of 16 superior durians used the modified CTAB method. The genomic DNA was amplified using 10 ISSR primers and then electrophoresed using 2% agarose gel. Data were analyzed using NTSYS PC version 2.02. The resulting allele has a relative size of 220 bp-1800 bp, with 87.9% are polymorphic. The similarity coefficient of 16 varieties was ranged between 0.54-0.88. All the superior durian varieties examined were different accessions so that each variety has the potential to be registered as a new variety of superior Indonesian durian. Specific alleles are found in G1, G3, G7, G8, and G13, which can be an identity of these varieties.

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“…Melon production in Indonesia from 2016 to 2018 increased (BPS 2019). Breeding efforts have been carried out to produce superior melon cultivars to meet the increasing needs of melon (Daryono et al 2019). The superior character to measure the melon quality is a combination of soluble sugars, organic acids, pigments, and volatile aromatic compounds (Kyriacou et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Characterization and expression of Cm-AAT1 gene encoding alcohol acyl-transferase in melon fruit (Cucumis melo L.) ‘Hikapel’

2020

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Abstract. Wibowo WA, Fatkhurohman MI, Daryono BS. 2020. Characterization and expression of Cm-AAT1 gene encoding alcohol acyl-transferase in melon fruit (Cucumis melo L.) ‘Hikapel’. Biodiversitas 21: 3041-3046. Melon (Cucumis melo L.) is one of the horticulture commodities that have high economic value and its needs increase continuously. Many new melon cultivars have been assembled to produce a higher quality melon. Melon 'Hikapel' developed by the Laboratory of Genetics and Breeding, Faculty of Biology UGM has distinctive character in the form of a strong aroma. This aroma is a complex mixture of various kinds of volatile compound. One of the main determinant compounds is a volatile ester, synthesized by the alcohol acyl-transferase enzyme encoded by the Cm-AAT1 gene. Characterization of Cm-AAT1 began with isolation of melon rinds to get total RNAs. Synthesis cDNA was conducted with oligo-dT primer, followed by detection of Cm-AAT1 using specific primers. A specific band was sequenced to perform phylogenetic tree. Gene expression from 4 melon cultivars, ‘Hikapel’, ‘Hikadi’, ‘Sun Lady’, and ‘Luna’ analysis was performed using relative quantitative Real-Time PCR. The results of this study showed that Cm-AAT1 owned not only by aromatic cultivars ‘Hikapel’ and ‘Hikadi’, but also owned by non-aromatic cultivars ‘Sun Lady’ and ‘Luna’. Phylogenetic analysis shows a high similarity between Cm-AAT1 on 'Hikapel' and 'Hikadi'. Gene expression analysis on 'Hikapel' increases as the process of fruit ripening during the storage period and it is in contrast to 'Hikadi' at decrease when the fruit began to enter the decay process on day 7th. Expression of Cm-AAT1 on ‘Hikapel’ was higher than ‘Hikadi’ at the peak of fruit maturity.

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Phenotypic and genetic stability of new Indonesian melon cultivar (Cucumis melo L. ‘Melonia’) based on ISSR markers

Cited by 22 publications

References 23 publications

Stability of watermelon phenotype characters (Citrullus lanatus (Thunb.) Matsum. & Nakai) from crossing ♀ ‘Putri Delima’ with ♂ ‘Maduri’

Stability of watermelon phenotype characters (Citrullus lanatus (Thunb.) Matsum. & Nakai) from crossing ♀ ‘Putri Delima’ with ♂ ‘Maduri’

Inter Simple Sequence Repeat molecular markers to reveal the genetic diversity of superior durian of Gunungpati, Semarang, Indonesia

Characterization and expression of Cm-AAT1 gene encoding alcohol acyl-transferase in melon fruit (Cucumis melo L.) ‘Hikapel’

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