Phenotypic and genomic analysis of Zymomonas mobilis ZM4 mutants with enhanced ethanol tolerance

Carreón-Rodríguez, Ofelia E.; Gutiérrez-Rı́os, Rosa Marı́a; Acosta, José Luís; Martı́nez, Alfredo; Cevallos, Miguel A.

doi:10.1016/j.btre.2019.e00328

Cited by 8 publications

(7 citation statements)

References 49 publications

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“…Recently, Pallach et al reported that one of the two cell surface exopolysaccharides in Z. mobilis , the galactose containing polymer (PS1), may have a crucial role in ethanol tolerance ( Pallach et al, 2018 ). Another study in two ethanol-tolerant mutants exhibited that clpP , spoT / relA , and clpB genes seem to contribute to the ethanol tolerance in Z. mobilis ( Carreon-Rodriguez et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Molecular mechanism of enhanced ethanol tolerance associated with hfq overexpression in Zymomonas mobilis

Tang

Wang

Yang

et al. 2022

Front. Bioeng. Biotechnol.

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Zymomonas mobilis is a promising microorganism for industrial bioethanol production. However, ethanol produced during fermentation is toxic to Z. mobilis and affects its growth and bioethanol production. Although several reports demonstrated that the RNA-binding protein Hfq in Z. mobilis contributes to the tolerance against multiple lignocellulosic hydrolysate inhibitors, the role of Hfq on ethanol tolerance has not been investigated. In this study, hfq in Z. mobilis was either deleted or overexpressed and their effects on cell growth and ethanol tolerance were examined. Our results demonstrated that hfq overexpression improved ethanol tolerance of Z. mobilis, which is probably due to energy saving by downregulating flagellar biosynthesis and heat stress response proteins, as well as reducing the reactive oxygen species induced by ethanol stress via upregulating the sulfate assimilation and cysteine biosynthesis. To explore proteins potentially interacted with Hfq, the TEV protease mediated Yeast Endoplasmic Reticulum Sequestration Screening system (YESS) was established in Z. mobilis. YESS results suggested that Hfq may modulate the cytoplasmic heat shock response by interacting with the heat shock proteins DnaK and DnaJ to deal with the ethanol inhibition. This study thus not only revealed the underlying mechanism of enhanced ethanol tolerance by hfq overexpression, but also provided an alternative approach to investigate protein-protein interactions in Z. mobilis.

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Section: Introductionmentioning

confidence: 99%

Molecular mechanism of enhanced ethanol tolerance associated with hfq overexpression in Zymomonas mobilis

Tang

Wang

Yang

et al. 2022

Front. Bioeng. Biotechnol.

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“…Ethanol toxicity becomes the primary problem for its production via microbial fermentation [40]. Numerous studies have been conducted to find, modify, and construct an optimal host with high tolerance to ethanol [9,41]. While the construction of an ethanol biosynthesis pathway in several heterologous hosts has been reported, the major obstacle limiting their achievement is due to the low tolerance of the host to ethanol toxicity.…”

Section: Discussionmentioning

confidence: 99%

Enhanced ethanol tolerance in Lysinibacillus sp.

2020

jabb

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Alcohol-tolerant microbes are the prime requirement for industrial-scale production of biofuels and beverages. Tolerance is a complex phenomenon that is achieved by mutational changes at several points in the genome. Since a network of genes and pathways are involved in adapting to ethanol tolerance, it is, therefore, more preferable to obtain ethanol tolerance phenotype by adaptive evolution. Adaptive evolution ensures genotypic changes which result in the evolution of a phenotypically tolerant strain. In the present work, Lysinibacillus sp. isolated from the estuarine area was subjected to adaptive evolution under ethanol stress that resulted in an increase in ethanol tolerance from 1.6% to 6.4%.

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“…Applying this method in Z. mobilis results in introducing two mutant strains with high capability to tolerate ethanol concentrations of more than 70–90 g/l. These two mutants were designated as ER79ag and ER79ap that both have a mutation in clpP , clpB , and spot/relA gene; however, the position of mutations in these two strains were different [ 124 ]. ClpB is an ATP-dependent chaperone that belongs to the Hsp100-family and is a member of the AAA (ATPase associated with diverse cellular activities) chaperone.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive network of stress-induced responses in Zymomonas mobilis during bioethanol production: from physiological and molecular responses to the effects of system metabolic engineering

Asefi,

Nouri,

Pourmohammadi

et al. 2024

Microb Cell Fact

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Nowadays, biofuels, especially bioethanol, are becoming increasingly popular as an alternative to fossil fuels. Zymomonas mobilis is a desirable species for bioethanol production due to its unique characteristics, such as low biomass production and high-rate glucose metabolism. However, several factors can interfere with the fermentation process and hinder microbial activity, including lignocellulosic hydrolysate inhibitors, high temperatures, an osmotic environment, and high ethanol concentration. Overcoming these limitations is critical for effective bioethanol production. In this review, the stress response mechanisms of Z. mobilis are discussed in comparison to other ethanol-producing microbes. The mechanism of stress response is divided into physiological (changes in growth, metabolism, intracellular components, and cell membrane structures) and molecular (up and down-regulation of specific genes and elements of the regulatory system and their role in expression of specific proteins and control of metabolic fluxes) changes. Systemic metabolic engineering approaches, such as gene manipulation, overexpression, and silencing, are successful methods for building new metabolic pathways. Therefore, this review discusses systems metabolic engineering in conjunction with systems biology and synthetic biology as an important method for developing new strains with an effective response mechanism to fermentation stresses during bioethanol production. Overall, understanding the stress response mechanisms of Z. mobilis can lead to more efficient and effective bioethanol production. Graphical Abstract

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Phenotypic and genomic analysis of Zymomonas mobilis ZM4 mutants with enhanced ethanol tolerance

Abstract: Highlights Z. mobilis ER79ag and ER79ap ethanol mutants were obtained by adaptive evolution. ER79ap had a better cell viability than the WT and ER79ap under ethanol stress. Mutants shared SNVs in clpP and spoT / relA , in addition ER79ap has a SNP in clpB . Mutant allele … Show more

Cited by 8 publications

References 49 publications

Molecular mechanism of enhanced ethanol tolerance associated with hfq overexpression in Zymomonas mobilis

Molecular mechanism of enhanced ethanol tolerance associated with hfq overexpression in Zymomonas mobilis

Enhanced ethanol tolerance in Lysinibacillus sp.

Comprehensive network of stress-induced responses in Zymomonas mobilis during bioethanol production: from physiological and molecular responses to the effects of system metabolic engineering

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