Phenotypic and genomic characterization of pathogenic Providencia rettgeri from kuruma shrimp Marsupenaeus japonicus

Abstract

Providencia rettgeri has been recognized as a zoonotic pathogen of humans and aquaculture animals and has become a global public health concern. However, scarce information is available on the characterization of pathogenic P. rettgeri from kuruma shrimp Marsupenaeus japonicus. In the present study, a P. rettgeri isolate (KM4) was confirmed as a causative agent of red leg disease in cultured M. japonicus, which showed a median lethal dose (LD50) value of 5.01 × 105 CFU·ml−1 and had multiple resistances to aminoglycosides, sulfonamides, and tetracycline antimicrobials used in aquaculture. In addition, the whole genome of isolate KM4 was sequenced and found to consist of a single circular chromosome of 4,378,712 bp and a circular plasmid of 171,394 bp. The genome sequence analysis further revealed the presence of potential virulence and antibiotic resistance genes in isolate KM4, which probably rendered this isolate particularly virulent. To our knowledge, this is the first study to characterize P. rettgeri pathogens from kuruma shrimp infected with red leg disease. The findings of this study can provide novel insights into the presence and distribution of pathogenicity‐associated genes in shrimp‐pathogenic P. rettgeri.

Pathogenicity of Streptococcus iniae causing mass mortalities of yellow catfish (Tachysurus fulvidraco) and its induced host immune response

“…Whole genome sequencing has become widely used to visualize high-resolution features of bacterial pathogens (Cao et al, 2022). Recently, genomic information of many pathogenic S. iniae have been announced to confirm genomic diversity.…”

Susceptibility of kuruma shrimp to the infection with Decapod iridescent virus 1

“…However, due to the lack of biosecurity awareness in the rapid rise of intensive aquaculture, disease has become one of the main factors restricting the increase of shrimp and crab production. The kuruma shrimp, Penaeus japonicus, is one of the most essential cultured and captured shrimps in the world and is farmed in Southeast Asian nations such as China, Japan, Korea, Australia, and others (Hewitt and Duncan, 2001;Ren et al, 2020;Cao et al, 2022). Due to its excellent desiccation resistance, tasty flavor, and abundant nutritional value, it is regarded as one of the most commercially important species of the Penaeidae family (Zhang et al, 2022).…”