Phenotypic and genotypic characteristics of Brucella isolates from the Republic of Kazakhstan

Yespembetov, Bolat; Syrym, Nazym S.; Zinina, Nadezhda; Sarmykova, Makhpal; Konbayeva, Gulshat M; Basybekov, Sovetzhan Z.; Mussayeva, Assiya; Kanatbayev, Serik G.; Bazarbayev, Marat B.; Siyabekov, Sarsenbek T

doi:10.1007/s11250-019-01941-y

Cited by 9 publications

(6 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The high morbidity and large territory of Kazakhstan make it essential to describe the genetic diversity and distribution of genotypes among circulating strains of Brucella in order to support efforts for disease control. Previous reports have described the genetic diversity of Brucella isolated from animals in Kazakhstan (Shevtsov et al, 2015; Shevtsova et al, 2016; Daugaliyeva et al, 2018; Yespembetov et al, 2019). The purpose of the present study was to determine the genetic diversity of the strains infecting humans at the whole country scale.…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity of Brucella melitensis in Kazakhstan in Relation to World-Wide Diversity

et al. 2019

View full text Add to dashboard Cite

We describe the genetic diversity of 1327 Brucella strains from human patients in Kazakhstan using multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA). All strains were assigned to the Brucella melitensis East Mediterranean group and clustered into 16 MLVA11 genotypes, nine of which are reported for the first time. MLVA11 genotype 116 predominates (86.8%) and is present all over Kazakhstan indicating existence and temporary preservation of a “founder effect” among B. melitensis strains circulating in Central Eurasia. The diversity pattern observed in humans is highly similar to the pattern previously reported in animals. The diversity observed by MLVA suggested that the epidemiological status of brucellosis in Kazakhstan is the result of the introduction of a few lineages, which have subsequently diversified at the most unstable tandem repeat loci. This investigation will allow to select the most relevant strains for testing these hypotheses via whole genome sequencing and to subsequently adjust the genotyping scheme to the Kazakhstan epidemiological situation.

show abstract

Section: Introductionmentioning

confidence: 99%

Genetic Diversity of Brucella melitensis in Kazakhstan in Relation to World-Wide Diversity

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Similarly, the most frequently recorded B. abortus biovar is biovar 3 in the Republic of Kazakhstan. More than 90% of the overall B. abortus samples were isolated from the northern regions of East and West Kazakhstan [31]. Five kinds of hosts were observed in this population, and 65% of strains were from cattle.…”

Section: Discussionmentioning

confidence: 88%

Molecular Epidemiology Characteristics of Brucella abortus Strains in China and Their Relationships with World Lineages

Wang¹,

Zhao²,

Wang³

et al. 2020

Preprint

View full text Add to dashboard Cite

Brucella abortus is a facultative intracellular Gram-negative bacterium that causes chronic persistent infections in humans and livestock. In this study, conventional bio-typing, multiple-locus variable-number tandem repeat analysis (MLVA), and whole-genome sequencing-single-nucleotide polymorphism (WGS-SNP) were used to investigate the molecular epidemiology characteristics of Brucella abortus strains in China and their relationships to world lineages. A total of 100 strains were collected from 1953 to 2013, suggesting that B. abortus circulated in China in the past five decades. Moreover, most strains were mainly distributed in the northwest area. During this period, seven biovars were found, indicating that B. abortus displayed a high diversity of biovars and that the main epidemic areas are in the northern provinces. Strains have high genetic diversity, and bruce07 is the most helpful locus for genotyping of this population. Moreover, 17 MLVA-11 genotypes were found; 13 of them are of known genotypes and four are unassigned genotypes, indicating that B. abortus in this study had several geographic origins. Still, strains from unassigned genotypes may originate from China. Many shared MLVA-16 genotypes were observed in strains from the same provinces in Northern China, which confirmed a brucellosis outbreak within northern regions. WGS-SNP analysis showed that eight Chinese strains formed a ladder-like phylogram (C. Ⅶ) with strains from nine countries, including Uganda, Iraq, Russia, Georgia, Spain, Italy, Egypt, Mongolia, and China; strains were introduced to these countries from a single source. Notably, Chinese strains were closely related to strains from Russia and Mongolia; frequent animal (cattle) trade and exchanges may promote this process. We will provide new and insightful information for strength surveillance and control of B. abortus brucellosis in China.

show abstract

“…Similarly, the most frequently recorded B. abortus biovar is biovar 3 in the Republic of Kazakhstan. More than 90% of the overall B. abortus samples were isolated from the northern regions of East and West Kazakhstan [40]. Five kinds of hosts were observed in this population, and 65% of strains were from cattle.…”

Section: Discussionmentioning

confidence: 88%

Molecular Epidemiology Characteristics of Brucella Abortus Strains in China and Their Relationships With World Lineages

Wang

Zhao

Wang³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Brucella abortus is a facultative intracellular Gram-negative bacterium that causes chronic persistent infections in humans and livestock. In this study, conventional bio-typing, multiple-locus variable-number tandem repeat analysis (MLVA), and whole-genome sequencing-single-nucleotide polymorphism (WGS-SNP) were used to investigate the molecular epidemiology characteristics of Brucella abortus strains in China and their relationships to world lineages. Results A total of 100 strains were collected from 1953 to 2013, suggesting that B. abortus circulated in China in the past five decades. Moreover, most strains were mainly distributed in the Northwest areas, suggest that provinces in the Northwest were a dominant epidemic area of this disease. During this period, seven biovars were found, indicating that B. abortus had a high diversity of biovars and it is also a potential reason for the disease ongoing spread in the Northern provinces. Strains have high genetic diversity, and bruce07 is the most helpful locus for genotyping of this population. Moreover, 17 MLVA-11 genotypes were found; 13 of them are of known genotypes and four are unassigned genotypes, indicating that B. abortus in this study had several geographic origins. Still, strains from unassigned genotypes may originate from China. Many shared MLVA-16 genotypes were observed in strains from the same provinces in Northern China, which confirmed a B. abortus brucellosis outbreak within Northern regions. WGS-SNP analysis showed that eight Chinese strains formed a ladder-like phylogram (C. Ⅶ) with strains from nine countries, including Uganda, Iraq, Russia, Georgia, Spain, Italy, Egypt, Mongolia, and China; suggest that strains were introduced to these countries from a single source. Conclusions Chinese B. abortus strains had high biovars and genetic diversity as well as represent characteristics of multiple geographic origins, and B. abortus strains from several mainly epidemic areas were closely related to strains from Russia and Mongolia; frequent animal (cattle) trade and exchanges may promote this process. We will provide new and valuable information to strengthening surveillance and control of B. abortus brucellosis in China.

show abstract

Phenotypic and genotypic characteristics of Brucella isolates from the Republic of Kazakhstan

Cited by 9 publications

References 18 publications

Genetic Diversity of Brucella melitensis in Kazakhstan in Relation to World-Wide Diversity

Genetic Diversity of Brucella melitensis in Kazakhstan in Relation to World-Wide Diversity

Molecular Epidemiology Characteristics of Brucella abortus Strains in China and Their Relationships with World Lineages

Molecular Epidemiology Characteristics of Brucella Abortus Strains in China and Their Relationships With World Lineages

Contact Info

Product

Resources

About