Phenotypic and Genotypic Characteristics of
            <i>Streptococcus porcinus</i>
            Isolated from Human Sources

Duarte, Rafael Silva; Barros, Rosana Rocha; Facklam, Richard R.; Teixeira, Lúcia Martins

doi:10.1128/jcm.43.9.4592-4601.2005

Cited by 37 publications

(33 citation statements)

References 24 publications

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“…Therefore, based on some of these findings, it has been suggested that S. pseudoporcinus may be associated with sexually transmitted infections (STIs). S. pseudoporcinus is exquisitely susceptible to beta-lactam antibiotics, vancomycin, and trimethoprim-sulfamethoxazole but has a high rate of resistance to tetracycline (1,3,7). Although some isolates of S. pseudoporcinus are resistant to erythromycin and clindamycin, significantly higher rates of resistance have been observed with S. agalactiae (1,3,5,7).…”

Section: Discussionmentioning

confidence: 93%

“…In 2005, Duarte et al used randomly amplified polymorphic DNA-PCR and pulsed-field gel electrophoresis analyses to show that these human isolates differed from isolates obtained from animal sources, with the majority of the human strains containing NG1 antigen (3). Subsequently, analysis of 16S rRNA gene sequence data of several human isolates recovered from female genitourinary tract specimens phenotypically described as S. porcinus revealed the sequences to be Ͼ2% dissimilar to any other Streptococcus species and thus highly suggestive of a novel species (4).…”

Section: Discussionmentioning

confidence: 99%

“…pseudoporcinus is a facultative, nonmotile, Gram-positive coccus that produces smooth, round-to-oval, beta-hemolytic colonies on blood agar (Fig. 1) and may possess E, P, or NG1 antigen or be untypeable (1,3,5). Like S. agalactiae, S. pseudoporcinus produces large colonies (typically Ͼ0.5 mm) after 24 h of incubation (which is useful for differentiating beta-hemolytic streptococci prior to Lancefield antigen typing because it excludes the possibility of beta-hemolytic strains of members of the Streptococcus anginosus group, whose colonies are characteristically smaller [Յ0.5 mm]), is CAMP factor positive, and is bacitracin resistant, and both species are most commonly isolated from the female genitourinary tract (1, 2).…”

Section: Discussionmentioning

confidence: 99%

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The Brief Case: Too Beta To Be a “B”

et al. 2017

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A 29-year-old African-American woman was seen in her third pregnancy. In her first pregnancy, she experienced preterm labor and premature rupture of membranes with delivery of a previable infant at 5 months. Her second pregnancy ended in spontaneous abortion at 7 weeks. Current other medical conditions include obesity, chronic kidney disease, benign essential hypertension, and prediabetes. She has a history of abnormal cervical cytology smears and was treated with colposcopy and a loop electrosurgical excision procedure, which was followed by successive normal cytology exams. Because of her history of pregnancy complications and short cervical length (Ͻ2 cm), her current pregnancy was managed with placement of a prophylactic McDonald cerclage at the gestational age of 21 weeks. The cerclage was removed at gestational age 37 weeks. The mother was monitored for slow fetal growth in the third trimester that was suspected to be due to placental insufficiency. A vaginorectal culture taken at 39 weeks for Streptococcus agalactiae (group B Streptococcus) grew a betahemolytic organism with a wide zone of hemolysis (Fig. 1). Gram staining showed Gram-positive cocci in pairs and short chains (Fig. 1), and the organism was catalase negative and pyrrolidonyl arylamidase (PYR) negative and produced a weak positive reaction with the group B latex typing reagent in the PathoDx Strep grouping kit (Diagnostic Products Corporation, Los Angeles, CA, USA). Because the wide zone of beta-hemolysis was not consistent with S. agalactiae, the isolate was analyzed using Vitek MS matrix-assisted laser desorption-ionization time of flight mass spectrometry (MALDI-TOF MS) (bioMérieux, Durham, NC, USA), which returned a nonclinically validated identification of Streptococcus porcinus, with a confidence score of 99.9% generated using IVD Knowledge Base version 2.0 and Myla version 4 information management software. To corroborate the accuracy of the MALDI-TOF MS result, the 16S rRNA gene was amplified using PCR and the resultant DNA fragment sequenced. Sequence data were analyzed using BLAST (https://blast.ncbi.nlm.nih.gov [16S rRNA sequence database]) and yielded 99% sequence homology (1,443 nucleotides [nt]/1,458 nt) to the Streptococcus pseudoporcinus LQ 940-04 T strain. The next-best matches, all with 96.7% homology, were to S. porcinus 176 T (1,450 nt/1,499 nt), Streptococcus hongkongensis HKU30 T (1,370 nt/1,417 nt), and Streptococcus uberis JCM 5709 T (1,455 nt/1,505 nt). These data supported an identification of S. pseudoporcinus rather than S. porcinus.The patient required Cesarean delivery at the gestational age of 39.3 weeks after failed induction of labor for severe gestational hypertension/preeclampsia. In this case, there was no premature rupture of membranes, and no intrapartum antibiotics were administered. On gross examination, the placenta weighed 383 g (Ͻ10th percentile for Citation Gullett JC, Westblade LF, Green DA, Whittier S, Burd EM. 2017. The Brief Case: Too beta to be a "B." J Clin Microbiol 55:1604 -1607.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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The Brief Case: Too Beta To Be a “B”

et al. 2017

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“…Streptococcus viridans and Streptococcus mitis/oralis are commensal bacteria in the oral cavity and gastrointestinal tract 11. Other blood culture revealed the growth of Streptococcus porcinus , a member of beta hemolyticus streptococcus originating from genitourinary tract of female in reproductive age 12. Single bacterial growth from blood culture of polymicrobial IE may result from growth competition in blood agar media.…”

Section: Discussionmentioning

confidence: 99%

Right‐sided infective endocarditis in patients with uncorrected ventricular septal defect and patent ductus arteriosus: Two case reports

Sattwika

Hartopo

Anggrahini

et al. 2018

Clinical Case Reports

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Key Clinical MessageUncorrected left‐to‐right shunt congenital heart defect is a predisposing factor for infective endocarditis (IE), especially right‐sided IE which has different clinical manifestations and complications from left‐sided IE. Prompt diagnosis by means of transthoracic echocardiography and timely antibiotics management for IE are encouraged to prevent multiorgan failure and fatal pulmonary embolism.

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“…Besides, they considered a detailed analysis of gel data by using high-resolution densitometers and software. Three groups of bacterial isolates belonging to Acromobacter, Neisseriaceae and Providencia were analyzed by this method, and subsequently the strategy of 1-D protein electrophoresis was widely used for typing of a number of bacterial isolates such as Streptococcus porcinus, Bacillus thuringiensis, Helicobacter pylori, etc (Duarte et al, 2005;Konecka et al 2007;Costas et al, 1990). The Haemophilus influenzae proteome was analyzed by 2-DE and extended to three other members of the Haemophilus genus in 1997.…”

Section: Microbial Systematics and Epidemiologymentioning

confidence: 99%