Phenotypic and Genotypic Characterization of Coagulase Negative Staphylococci (CoNS) Other than<i>Staphylococcus epidermidis</i>Isolated from Ocular Infections

Makki, Abhijith; Sharma, Savitri; Duggirala, Aparna; Prashanth, K; Garg, Prashant; Das, Taraprasad

doi:10.1167/iovs.11-7777

Cited by 24 publications

(16 citation statements)

References 21 publications

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“…17 The results exhibited that except for fusidic acid and polypeptide antibiotics, the resistance of Staphylococcus epidermidis to other antibiotics was even higher than that of MRSA, the severity of which should not be ignored as a result. As an opportunistic pathogen, Enterococcus led to increasing infections every year, and its drug resistance has become extremely serious according to the results in our hospital.…”

Section: Discussionmentioning

confidence: 96%

Analysis of some common pathogens and their drug resistance to antibiotics

Peng¹,

Ren²,

Ma³

et al. 2012

Pak J Med Sci

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Objective: To investigate the common bacterial resistance of clinical isolates in our hospital in the second half of 2011. Methodology: Pathogens isolated from clinical samples in the second half of 2011 were analyzed and categorized to perform susceptibility tests. Results: In the gram-negative bacteria, Enterobacteriaceae and non-fermenting gram-negative bacilli accounted for 55.89% and 34.51%. In the gram-positive bacteria, Staphylococcus aureus, Coagulase-negative staphylococci, Enterococcus, Strptococcus pneumonia accounted for 32.85%, 40.39%, 12.41% and 10.22%, respectively. Other species accounted for 4.14%. Klebsiella pneumonia and Pseudomonas aeruginosa were sensitive to cepoperazon, cefepime and imipenem. However,Acinetobacter baumannii was more sensitive to carbapenems antibiotics, which was followed by fourth generation cephalosporins. Klebsiella pneumoniae was extremely sensitive to amikacin, cefepime and imipenem, but was resistant to ampicillin. The detection rates of the broad-spectrum Escherichia coli, Pseudomonasaeruginosa and Klebsiella pneumoniae were 54.51%, 52.08% and 38.65%. The gram negative bacilli were the prevalent clinical pathogens in our hospital in the second half of 2011. Conclusion: The drug resistance of pathogenic bacteria has increased significantly recently, thus the surveillance of antibacterial agents is necessary, and rational use of antibiotic will be urgently needed to reduce the production and dissemination of drug resistant strains.

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Section: Discussionmentioning

confidence: 96%

Analysis of some common pathogens and their drug resistance to antibiotics

Peng¹,

Ren²,

Ma³

et al. 2012

Pak J Med Sci

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“…Even commercial identification systems for CoNS such as API STAPH are reported to have problems in identification as exemplified by a study where four isolates which were identified as S. haemolyticus by ITS-PCR were misidentified as S. epidermidis, S. hominis and S. warneri (25,26). Molecular methods, including 16S rDNA based identification is highly reproducible, unambiguous, rapid, accurate and efficient for the epidemiological analysis of medically significant bacteria and also in categorizing them up to species and subspecies level (25). Hence in the current study, identification of the isolates was made by combining the results of biochemical tests, MPCR and 16S rDNA sequencing.…”

Section: Discussionmentioning

confidence: 99%

Studies on prevalence of biofilm associated genes and primary observation on sasX gene in clinical isolates of coagulase negative staphylococci (CoNS)

Soumya

Mathew

Sugathan

et al. 2016

APMIS

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Coagulase negative staphylococci (CoNS) are nosocomial pathogens that cause indwelling medical device associated infections due to its biofilm forming potential and multiple antibiotic resistance. The current study focused on species identification, antibiotic resistance profile and molecular basis of biofilm formation and attachment of CoNS isolated from clinical samples. Along with this, molecular screening for mecA and newly identified surface colonization protein encoded by sasX gene was also conducted. S. epidermidis (n = 19, 47%) was identified as the most prevalent CoNS species and very interestingly two biofilm forming, mecA positive S. epidermidis isolates were found to carry all the biofilm associated genes screened in this study, which indicates its potential to form the strong biofilm. Another novel observation of the study is the detection of sasX gene in one biofilm positive S. epidermidis isolate. The study also identified one doxycycline resistant mecA positive, multidrug resistant S. haemolyticus isolate. In conclusion, the study signifies the existence of multiple biofilm related genes, multidrug resistance and the presence of sasX gene among clinical isolates of CoNS.

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“…S. xylosus is a coagulase-negative gram-positive staphylococcal species and a common skin commensal bacterium of humans and other mammals 6 . Some cases of S. xylosus infection, including erythema nodosum 7 , pyelonephritis 8 , and corneal infections 9 occur in humans. In laboratory mice, S. xylosus has been associated with spontaneous dermatitis in both NOS2 −/− mice 10 and athymic nude mice 11 and with conjunctivitis in NADPH oxidase −/− mice 12 , implying that S. xylosus is an opportunistic pathogen.…”

Section: Introductionmentioning

confidence: 99%

The resident pathobiont Staphylococcus xylosus in Nfkbiz-deficient skin accelerates spontaneous skin inflammation

Kim

Lee

Yang

et al. 2017

Sci Rep

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IκBζ, which is encoded by the Nfkbiz gene, is a member of the nuclear IκB family of proteins that act as transcriptional regulators via association with NF-κB. Nfkbiz-deficient (Nfkbiz −/−) mice develop spontaneous dermatitis; however, the underlying mechanism has yet to be elucidated. In our study, we found higher skin pathology scores and more serum IgE antibodies and trans-epidermal water loss in Nfkbiz −/− than in Nfkbiz-sufficient (Nfkbiz +/−) mice. There was also greater expansion of IFN-γ-, IL-17A-, and IL-22-secreting CD4+ T cells and of IL-17A-secreting γδ+ T cells in the skin of Nfkbiz −/− mice than in with Nfkbiz +/− mice. Pyrosequencing analysis showed decreased diversity of resident bacteria and markedly expanded Staphylococcus (S.) xylosus in the skin of Nfkbiz −/− mice. Oral administration of antibiotics including cephalexin and enrofloxacin ameliorated skin inflammation. Topical application of S. xylosus also resulted in the expansion of IL-17A-secreting CD4+ T cells along with high levels of pro-inflammatory cytokines and chemokines in the skin of Nfkbiz −/− mice. The expansion of commensal S. xylosus may be one cause of skin dysbiosis in Nfkbiz −/− mice and suggests that the Nfkbiz gene may play a regulatory role in the microbiota-skin immunity axis.

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Phenotypic and Genotypic Characterization of Coagulase Negative Staphylococci (CoNS) Other thanStaphylococcus epidermidisIsolated from Ocular Infections

Cited by 24 publications

References 21 publications

Analysis of some common pathogens and their drug resistance to antibiotics

Analysis of some common pathogens and their drug resistance to antibiotics

Studies on prevalence of biofilm associated genes and primary observation on sasX gene in clinical isolates of coagulase negative staphylococci (CoNS)

The resident pathobiont Staphylococcus xylosus in Nfkbiz-deficient skin accelerates spontaneous skin inflammation

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