Phenotypic and genotypic characterization of Staphylococcus aureus isolated from raw camel milk samples

Shuiep, E. S.; Kanbar, T.; Eissa, Nawara; Alber, Julia M.; Lämmler, Christoph; Zschöck, M.; Zubeir, Ibtisam E. M. El; Weiß, R.

doi:10.1016/j.rvsc.2008.07.011

Cited by 30 publications

(20 citation statements)

References 37 publications

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“…Additionally, handling of milk in different plastic containers and the use of unclean water for washing of milking equipment may cause contamination of milk. Similarly, Rahimi and Alian, [20] in Iran, Birhanu et al [21] in north eastern Ethiopia, Shuiep et al [22] in Sudan and Aydin et al [23] in Turkey reported 11.0%, 7.14%, 8.8% and 10.2%, respectively.…”

Section: Prevalence Of Staphylococcus Aureus In Raw Camel Milkmentioning

confidence: 73%

Prevalence and Contamination Level of Staphylococcus aureus in Raw Camel Milk and Associated Risk Factors in Jigjiga District, Eastern Ethiopia

Serda¹,

Bekele²,

Abebe³

2018

J Vet Sci Technol

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A cross sectional study was conducted from August, 2014 to February 2015 aimed to determine the prevalence of Staphylococcus aureus in raw camel milk and to assess risk factors. The study was conducted on a total of 384 bulk milk samples from three critical points. For this study both laboratory and questionnaire based data were collected. The overall prevalence of S. aureus was found to be 11.45% (44/384). The frequency of isolation of S. aureus varied between sources of sample and ranged from 7-15%. The prevalence of S. aureus was 7.03% (9/128), 11.71% (15/128) and 15% (20/128) from household, primary collection centers and selling sites, respectively. The raw camel milk samples were contaminated and the S. aureus counts markedly variable among samples at different sampling points. Mean S. aureus count was found to be 4.2 × 10 4 CFU/ml and the mean count of the samples at household was 8.9 × 10 2 CFU/ml, 9.9 × 10 3 CFU/ml at primary collection centers and 1.1 × 10 5 CFU/ml at selling sites. The difference was not statistically significant between the sampling points (p>0.05). In the study area, camel milk is consumed (100%) in its raw state without being subjected to any sort of processing treatment. The outcome of the present results suggests that, the examined raw camel milk samples were produced and handled under poor hygienic conditions with high health risk to the consumers. Consumption of raw camel milk should be of major concern from public health point of view. This study recommends for urgent development and adaptation of feasible and sustainable interventions to improve the camel milk hygiene and safety in study area and to mitigate staphylococcal food poisoning (SFP) in the region.

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Section: Prevalence Of Staphylococcus Aureus In Raw Camel Milkmentioning

confidence: 73%

Prevalence and Contamination Level of Staphylococcus aureus in Raw Camel Milk and Associated Risk Factors in Jigjiga District, Eastern Ethiopia

Serda¹,

Bekele²,

Abebe³

2018

J Vet Sci Technol

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“…Analysis of many S. aureus isolates from sheep, cows and poultry demonstrated that this cluster is also present in all the animal isolates tested so far. For SSL7 specifically, it is described that the gene encoding for this protein is found in 90% of clinical isolates (Monecke et al ., 2008) and is present in all of the tested animal isolates from chicken, sheep, goat, rabbit and camel (Smyth et al ., 2007; Shuiep et al ., 2008). The high prevalence of ssl7 in S. aureus strains and the production of SSL7 following host contact strongly suggest a beneficial role for SSL7 in bacterial survival within the host environment.…”

Section: Discussionmentioning

confidence: 99%

Functional basis for complement evasion by staphylococcal superantigen-like 7

Bestebroer

Aerts

Rooijakkers

et al. 2010

Cellular Microbiology

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SummaryThe human pathogen Staphylococcus aureus has a plethora of virulence factors that promote its colonization and survival in the host. Among such immune modulators are staphylococcal superantigen-like (SSL) proteins, comprising a family of 14 small, secreted molecules that seem to interfere with the host innate immune system. SSL7 has been described to bind immunoglobulin A (IgA) and complement C5, thereby inhibiting IgAFcaRI binding and serum killing of Escherichia coli. As C5a generation, in contrast to C5b-9-mediated lysis, is crucial for immune defence against staphylococci, we investigated the impact of SSL7 on staphylococcal-induced C5a-mediated effects. Here, we show that SSL7 inhibits C5a generation induced by staphylococcal opsonization, slightly enhanced by its IgA-binding capacity. Moreover, we demonstrate a strong protective activity of SSL7 against staphylococcal clearance in human whole blood. SSL7 strongly inhibited the C5a-induced phagocytosis of S. aureus and oxidative burst in an in vitro whole-blood inflammation model. Furthermore, we found that SSL7 affects all three pathways of complement activation and inhibits the cleavage of C5 by interference of its binding to C5 convertases. Finally, SSL7 effects were also demonstrated in vivo. In a murine model of immune complex peritonitis, SSL7 abrogated the C5a-driven influx of neutrophils in mouse peritoneum.

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“…MSSA strains have been identified in very diverse types of food in Africa in very different percentages ( Table 2 ) [ 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 , 106 ]. The rates detected in raw meat, meat products and cooked meat have been from 3% to 81.8%.…”

Section: S Aureus In Food In Africamentioning

confidence: 99%

Staphylococcus aureus in Animals and Food: Methicillin Resistance, Prevalence and Population Structure. A Review in the African Continent

et al. 2016

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The interest about Staphylococcus aureus (S. aureus) and methicillin resistant S. aureus (MRSA) in livestock, and domestic and wild animals has significantly increased. The spread of different clonal complexes related to livestock animals, mainly CC398, and the recent description of the new mecC gene, make it necessary to know more about the epidemiology and population structure of this microorganism all over the world. Nowadays, there are several descriptions about the presence of S. aureus and/or MRSA in different animal species (dogs, sheep, donkeys, bats, pigs, and monkeys), and in food of animal origin in African countries. In this continent, there is a high diversity of ethnicities, cultures or religions, as well as a high number of wild animal species and close contact between humans and animals, which can have a relevant impact in the epidemiology of this microorganism. This review shows that some clonal lineages associated with humans (CC1, CC15, CC72, CC80, CC101, and CC152) and animals (CC398, CC130 and CC133) are present in this continent in animal isolates, although the mecC gene has not been detected yet. However, available studies are limited to a few countries, very often with incomplete information, and many more studies are necessary to cover a larger number of African countries.

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Phenotypic and genotypic characterization of Staphylococcus aureus isolated from raw camel milk samples

Cited by 30 publications

References 37 publications

Prevalence and Contamination Level of Staphylococcus aureus in Raw Camel Milk and Associated Risk Factors in Jigjiga District, Eastern Ethiopia

Prevalence and Contamination Level of Staphylococcus aureus in Raw Camel Milk and Associated Risk Factors in Jigjiga District, Eastern Ethiopia

Functional basis for complement evasion by staphylococcal superantigen-like 7

Staphylococcus aureus in Animals and Food: Methicillin Resistance, Prevalence and Population Structure. A Review in the African Continent

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