Phenotypic and genotypic characterization of biofilm producing clinical coagulase negative staphylococci from Nepal and their antibiotic susceptibility pattern

Manandhar, Sarita; Singh, Anjana; Varma, Ajit; Pandey, Shanti; Shrivastava, Neeraj

doi:10.1186/s12941-021-00447-6

Cited by 12 publications

(7 citation statements)

References 47 publications

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“…The biofilm of CoNS is composed of the layer of an extracellular polymeric substance called polysaccharide intercellular adhesion (PIA) matrix, which is encoded by ica operon ( icaA, icaB icaC and icaD genes) [ 23 ]. The PIA mediates the intercellular adherence of bacteria and the multilayer accumulation of the biofilm, making them resistant to antimicrobial agents and to the immunological response of the host [ 23 , 24 , 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

Persistent Coagulase-Negative Staphylococcal Bacteremia in Neonates: Clinical, Microbiological Characteristics and Changes within a Decade

et al. 2022

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Atypical outbreaks of persistent coagulase-negative staphylococci (CoNS) bacteremias, defined as three or more consecutive positive blood cultures with the same CoNS species, at least 48 h apart, have been reported in neonatal intensive-care units (NICUs). Our aim was to describe the profile of these cases in our NICU over a two-year period with the objective of assessing possible changes within a decade. Demographics, clinical and microbiological data were recorded for all CoNS bacteremias in our tertiary NICU during 2016–2017 and compared with the results of the same study in 2006–2007. Fifty-six cases of CoNS sepsis were recorded. Fourteen (25%) of them were persistent. There were no significant differences in demographic and clinical characteristics between cases with persistent vs. non-persistent bacteremia. Staphylococcus epidermidis was the most common species. In logistic regression analysis, biofilm production (β = 2.464, p = 0.04) was the most significant determinant for the development of persistent CoNS bacteremia. Our isolates were less likely to produce biofilm and carry ica operon as compared to those of 2006–2007. The cases of persistent CoNS sepsis have decreased within a decade, which could be attributed to the implementation of intensive infection control practices. Biofilm production remains the most important risk factor.

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Section: Introductionmentioning

confidence: 99%

Persistent Coagulase-Negative Staphylococcal Bacteremia in Neonates: Clinical, Microbiological Characteristics and Changes within a Decade

et al. 2022

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“…Resistance to ciprofloxacin was also associated with resistance to gentamicin and oxacillin, while oxacillin resistance was associated with chloramphenicol and tetracycline resistance. According to a study by Manandhar et al (2021) Manandhar et al (2021). An association between erythromycin and clindamycin resistance was shown for nearly 30% of the isolates.…”

Section: Discussionmentioning

confidence: 99%

Analysis of phenotypic and genotypic antibiotic resistance of food isolates of Staphylococcus spp.

Piekarska-Radzik¹,

Klewicka²,

Otlewska³

2022

Acta Sci Pol Technol Aliment

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Background. Staphylococcus bacteria are potentially pathogenic species that are more and more often isolated from the natural environment. They are often responsible for the spoilage of food products and for food poisoning in consumers. The aim of this study was to analyse the phenotypic and genotypic antibiotic resistance of food isolates of the genus Staphylococcus. Materials and methods. The biological material consisted of 5 coagulase-negative strains of the genus Staphylococcus isolated from food (radish sprouts, fresh milk and a dietary supplement) and a reference strain, Staphylococcus epidermidis DSMZ 3270. Phenotypic antibiotic resistance was examined using the disc method, while genotypic antibiotic resistance assessment was examined using Real-Time PCR. Results. All of the tested isolates turned out to be resistant to 6 antibiotics: erythromycin, gentamycin, rifampicin, linezolid, tigecycline and methicillin. On this basis, the isolates were classified as multi-drug-resistant strains. For all 5 isolates and Staphylococcus epidermidis DSMZ 3270, only 2 common genes present in the DNA (plasmid and/or genomic) were identified: tet(L) and int(Tn916/Tn1545). However, none of them had the mec(A) gene. Conclusion. On this basis, the isolates were classified as multi-drug-resistant strains. The study confirms the increasing prevalence of multi-drug-resistant bacteria of the genus Staphylococcus in the environment.

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“…Biofilm formation is considered to be one of the major batteries of saprophytic microbiota to become opportunistic pathogen (Kloos and Bannerman 1994 ; Mertens and Ghebremedhin 2013 ; Manandhar et al 2021 ). Some of the bacteria, such as coagulase-negative staphylococci (CNS) ( Staphylococcus epidermis and S .…”

Section: Detection Of Biofilm Formationmentioning

confidence: 99%

“…Some of the bacteria, such as coagulase-negative staphylococci (CNS) ( Staphylococcus epidermis and S . aureus ) possess the ability to aggregate and form biofilms by virtue of their secreted mucoid extracellular polymeric substance called polysaccharide intercellular adhesion (PIA) matrix encoded by the icaA , icaD icaB , and icaC genes (Arciola et al 2014 ; Manandhar et al 2021 ). On the other hand, the biofilm-associated protein ( bap ) found in A. baumannii is both a cell surface protein and a virulence factor.…”

Section: Detection Of Biofilm Formationmentioning

confidence: 99%

Biofilm-mediated infections by multidrug-resistant microbes: a comprehensive exploration and forward perspectives

Zafer,

Mohamed,

Ibrahim

et al. 2024

Arch Microbiol

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A biofilm is a collection of microorganisms organized in a matrix of extracellular polymeric material. Biofilms consist of microbial cells that attach to both surfaces and each other, whether they are living or non-living. These microbial biofilms can lead to hospital-acquired infections and are generally detrimental. They possess the ability to resist the human immune system and antibiotics. The National Institute of Health (NIH) states that biofilm formation is associated with 65% of all microbial illnesses and 80% of chronic illnesses. Additionally, non-device-related microbial biofilm infections include conditions like cystic fibrosis, otitis media, infective endocarditis, and chronic inflammatory disorders. This review aims to provide an overview of research on chronic infections caused by microbial biofilms, methods used for biofilm detection, recent approaches to combat biofilms, and future perspectives, including the development of innovative antimicrobial strategies such as antimicrobial peptides, bacteriophages, and agents that disrupt biofilms.

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Phenotypic and genotypic characterization of biofilm producing clinical coagulase negative staphylococci from Nepal and their antibiotic susceptibility pattern

Cited by 12 publications

References 47 publications

Persistent Coagulase-Negative Staphylococcal Bacteremia in Neonates: Clinical, Microbiological Characteristics and Changes within a Decade

Persistent Coagulase-Negative Staphylococcal Bacteremia in Neonates: Clinical, Microbiological Characteristics and Changes within a Decade

Analysis of phenotypic and genotypic antibiotic resistance of food isolates of Staphylococcus spp.

Biofilm-mediated infections by multidrug-resistant microbes: a comprehensive exploration and forward perspectives

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