Phenotypic and Genotypic Characterization of <i>Escherichia coli</i> and <i>Salmonella enterica</i> from Dairy Cattle Farms in the Wakiso District, Uganda: A Cross-Sectional Study

Ball, Takiyah; Monte, Daniel Farias Marinho do; Aı̈dara-Kane, Awa; Matheu-Alvarez, Jorge; Ru, Hongyu; Thakur, Siddhartha; Horovitz, Joy; Ejobi, Francis; Lacher, David W.; Fedorka–Cray, Paula J.

doi:10.1089/fpd.2018.2528

Cited by 16 publications

(6 citation statements)

References 20 publications

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“…[37] also reported the prevalence of a higher number of tetracycline resistance genes (11/15) in E. coli associated with dairy farm. On the other hand, the prevalence of SHV and ereA genes was found comparatively low in this study as reported elsewhere [47,48].…”

Section: Discussionsupporting

confidence: 64%

Antibiotic-resistant Escherichia coli and Salmonella spp. associated with dairy cattle and farm environment having public health significance

et al. 2019

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Aim: The present study was carried out to determine load of total bacteria, Escherichia coli and Salmonella spp. in dairy farm and its environmental components. In addition, the antibiogram profile of the isolated bacteria having public health impact was also determined along with identification of virulence and resistance genes by polymerase chain reaction (PCR) under a one-health approach. Materials and Methods: A total of 240 samples of six types (cow dung - 15, milk - 10, milkers' hand wash - 10, soil - 10 water - 5, and vegetables - 10) were collected from four dairy farms. For enumeration, the samples were cultured onto plate count agar, eosin methylene blue, and xylose-lysine deoxycholate agar and the isolation and identification of the E. coli and Salmonella spp. were performed based on morphology, cultural, staining, and biochemical properties followed by PCR. The pathogenic strains of E. coli stx1, stx2, and rfbO157 were also identified through PCR. The isolates were subjected to antimicrobial susceptibility test against 12 commonly used antibiotics by disk diffusion method. Detection of antibiotic resistance genes ereA, tetA, tetB, and SHV were performed by PCR. Results: The mean total bacterial count, E. coli and Salmonella spp. count in the samples ranged from 4.54±0.05 to 8.65±0.06, 3.62±0.07 to 7.04±0.48, and 2.52±0.08 to 5.87±0.05 log colony-forming unit/g or ml, respectively. Out of 240 samples, 180 (75%) isolates of E. coli and 136 (56.67%) isolates of Salmonella spp. were recovered through cultural and molecular tests. Among the 180 E. coli isolates, 47 (26.11%) were found positive for the presence of all the three virulent genes, of which stx1 was the most prevalent (13.33%). Only three isolates were identified as enterohemorrhagic E. coli. Antibiotic sensitivity test revealed that both E. coli and Salmonella spp. were found highly resistant to azithromycin, tetracycline, erythromycin, oxytetracycline, and ertapenem and susceptible to gentamycin, ciprofloxacin, and imipenem. Among the four antibiotic resistance genes, the most observable was tetA (80.51-84.74%) in E. coli and Salmonella spp. and SHV genes were the lowest one (22.06-25%). Conclusion: Dairy farm and their environmental components carry antibiotic-resistant pathogenic E. coli and Salmonella spp. that are potential threat for human health which requires a one-health approach to combat the threat.

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Section: Discussionsupporting

confidence: 64%

Antibiotic-resistant Escherichia coli and Salmonella spp. associated with dairy cattle and farm environment having public health significance

et al. 2019

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“…The detection of CTX-M-27 in Escherichia coli patient isolates has been increasing, and is especially alarming because of its presence in clonal groups such as ST10, ST69 and ST131 [3][4][5][6]. In particular, E. coli ST131 isolates are the primary hosts responsible for CTX-M-27 transmission in E. coli from companion animals, the environment, farm animals and animal products [3,[7][8][9][10][11][12][13]. IncF plasmids and transposons are frequently associated with transfer of bla CTX-M-27 in E. coli and are frequently associated with IncF plasmids and transposons [14,15].…”

Section: Introductionmentioning

confidence: 99%

A Novel Structure Harboring blaCTX-M-27 on IncF Plasmids in Escherichia coli Isolated from Swine in China

et al. 2021

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The aim of this study was to elucidate the prevalence of blaCTX-M-27-producing Escherichia coli and transmission mechanisms of blaCTX-M-27 from swine farms in China. A total of 333 E. coli isolates were collected from two farms from 2013 to 2016. Thirty-two CTX-M-27-positive E. coli were obtained, and all were multidrug-resistant. Pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) profiles indicated a wide range of strain types that carried blaCTX-M-27, and the sequence type ST10 predominated. Conjugation, replicon typing, S1-PFGE and hybridization experiments confirmed that 28 out of 32 CTX-M-27 positive isolates carried blaCTX-M-27 genes on plasmids F18:A-:B10 (16) and F24:A-:B1 (12).The blaCTX-M-27 genes for 24 isolates were transmitted by plasmids with sizes ranging from 40 to 155 kb. A comparative analysis with blaCTX-M-27-plasmids indicated that the tra-trb region of F24:A-:B1 plasmids was destroyed by insertion of a complex region (eight isolates) and a novel structure containing blaCTX-M-27 in the F18:A-:B10 plasmids (12 isolates). The novel structure increased the stability of the blaCTX-M-27 gene in E. coli. This study indicated that the predominant vehicle for blaCTX-M-27 transmission has diversified over time and that control strategies to limit blaCTX-M-27 transmission in farm animals are necessary.

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“…The bla CTX‐M‐27 , a variant resulting from a single nucleotide substitution of bla CTX‐M‐14 , shows increased enzymatic activity against ceftazidime and has recently increased in frequency within Europe and Asia (Bevan et al., 2017 ; Cantón, González‐Alba & Galán, 2012 ). In Africa, few occurrences of bla CTX‐M‐27 have been detected in poultry and cattle (Ayeni et al., 2020 ; Ball et al., 2019 ). Given the current global trend of bla CTX‐M‐27 increasing in frequency relative to the bla CTX‐M‐15 variant, future studies in this area should continue to track the molecular epidemiology and frequency of these two gene variants (Bevan et al., 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance patterns and characterisation of emerging beta‐lactamase‐producing Escherichia coli in camels sampled from Northern Kenya

Akunda

Kariuki

Matulis

et al. 2023

Veterinary Medicine & Sci

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Background: Animal husbandry practices in different livestock production systems and increased livestock-wildlife interactions are thought to be primary drivers of antimicrobial resistance (AMR) in Arid and Semi-Arid Lands (ASALs). Despite a tenfold increase in the camel population within the last decade, paired with widespread use of camel products, there is a lack of comprehensive information concerning beta-lactamase-producing Escherichia coli (E. coli) within these production systems.Objectives: Our study sought to establish an AMR profile and to identify and characterise emerging beta-lactamase-producing E. coli isolated from faecal samples obtained from camel herds in Northern Kenya. Methods:The antimicrobial susceptibility profiles of E. coli isolates were established using the disk diffusion method, with beta-lactamase (bla) gene PCR product sequencing performed for phylogenetic grouping and genetic diversity assessments.Results: Here we show, among the recovered E. coli isolates (n = 123), the highest level of resistance was observed for cefaclor at 28.5% of isolates, followed by cefotaxime at 16.3% and ampicillin at 9.7%. Moreover, extended-spectrum beta-lactamase (ESBL)producing E. coli harbouring the bla CTX-M-15 or bla CTX-M-27 genes were detected in 3.3% of total samples, and are associated with phylogenetic groups B1, B2 and D. Multiple variants of non-ESBL bla TEM genes were detected, the majority of which were the bla TEM-1 and bla TEM-116 genes.Conclusions: Findings from this study shed light on the increased occurrence of ESBLand non-ESBL-encoding gene variants in E. coli isolates with demonstrated multidrug resistant phenotypes. This study highlights the need for an expanded One Health approach to understanding AMR transmission dynamics, drivers of AMR development, and appropriate practices for antimicrobial stewardship in camel production systems within ASALs.

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Phenotypic and Genotypic Characterization of Escherichia coli and Salmonella enterica from Dairy Cattle Farms in the Wakiso District, Uganda: A Cross-Sectional Study

Cited by 16 publications

References 20 publications

Antibiotic-resistant Escherichia coli and Salmonella spp. associated with dairy cattle and farm environment having public health significance

Antibiotic-resistant Escherichia coli and Salmonella spp. associated with dairy cattle and farm environment having public health significance

A Novel Structure Harboring blaCTX-M-27 on IncF Plasmids in Escherichia coli Isolated from Swine in China

Antimicrobial resistance patterns and characterisation of emerging beta‐lactamase‐producing Escherichia coli in camels sampled from Northern Kenya

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