Phenotypic and genotypic characterizations of Escherichia coli Isolated from veal meats and butchers’ shops in Mosul city, Iraq

Othman, Shaker M.; Sheet, Omar H.; Al-Sanjary, Raad

doi:10.33899/ijvs.2022.133819.2306

Cited by 3 publications

(3 citation statements)

References 26 publications

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“…Thus, E. coli strains resistant to antimicrobial drugs are widespread in the production environment of dairy enterprises in Kazakhstan and can seriously threaten public health because such bacteria can carry and transmit genes encoding determinants of antibiotic resistance [ 46 ]. Molecular studies of E. coli isolates in the framework of this work confirmed the presence of eaeA virulence and resistance genes to β-lactam, sulfonamide, and quinolone groups.…”

Section: Discussionmentioning

confidence: 99%

Contamination of Kazakhstan cheeses originating from Escherichia coli and its resistance to antimicrobial drugs

Kuzeubayeva,

Ussenbayev,

Aydin

et al. 2024

Vet World

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Background and Aim: Escherichia coli, a commensal intestine bacterium of vertebrates, is widely distributed in the environment and indicates the microbiological quality of food products in relation to coliforms. In addition, virulent strains, particularly E. coli O157:H7, cause outbreaks of toxic infections caused by consuming dairy products. Because food safety studies regarding E. coli have not been conducted in Central Asia, this research aimed to study the characteristics of contamination, microbiological and genotypic properties, and resistance to antimicrobial agents of E. coli strains that contaminate various types of commercialized cheeses originating from Kazakhstan. Materials and Methods: In retail outlets, 207 samples of three types of cheese produced by 22 industrial and eight small enterprises in the central, eastern, southern, and northern regions of Kazakhstan were selected in 2020–2023. E. coli contamination was examined using standard microbiological, mass spectrometric, and molecular genetic methods. The discodiffuse European Committee on Antimicrobial Susceptibility Testing method was used to test the resistance of the identified E. coli isolates (65/207; 31.4%) to 20 antibacterial drugs. The Shiga toxin-producing E. coli (VT1 and VT2) and E. coli O157:H7 (eae) genes were investigated in all E. coli isolates using multiplex polymerase chain reaction. Results: An average of 31.4% samples of commercial Kazakhstani cheeses of various types were found to be contaminated with E. coli in almost all geographical regions of Kazakhstan, regardless of the productivity of the dairy enterprises. Soft cheeses produced by small farms (80% of samples) packaged at the retail site (100%) were the most contaminated with E. coli. The microbiological index (colony-forming unit/g) was unsatisfactory and unsuitable in 6.2% of such cheese samples. For the first time in Central Asia, the enteropathogenic strain E. coli O157:H7 was detected in 0.5% of cheese samples. E. coli isolates from cheese samples were resistant to 65% of antibacterial drugs and contained resistance genes to β-lactams, sulfonamides, and quinolones groups. At the same time, 25% of the E. coli isolates were multi-resistant to three or more antimicrobial agents. Conclusion: The high level of contamination caused by multi-antibiotic resistant E. coli strains, including pathogenic pathogens, poses a risk to public health and highlights the need for further research on the monitoring and control of coliform enteropathogens in food products. Keywords: antibiotic resistance, Escherichia coli O157:H7, Kazakhstan cheese, microbial contamination.

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Section: Discussionmentioning

confidence: 99%

Contamination of Kazakhstan cheeses originating from Escherichia coli and its resistance to antimicrobial drugs

Kuzeubayeva,

Ussenbayev,

Aydin

et al. 2024

Vet World

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“…The fifth dilution was taken and a sensitivity test was performed on Mueller-Hinton Agar medium using the drilling method and injecting a ratio of (0.5) into the hole and after incubating in the incubator at 37 degrees Celsius for 24 hours (Figure 3). The test result was read by reading the zoom with a result of 16 mm (Salman et al, 2022;Othman et al, 2023).…”

Section: Materials and Methods Materialsmentioning

confidence: 99%

Effect of Berberine on Some Histopathological, Enrofloxacin Residue and Aniti-Diarrheaginic Escherichia coli in Broilar Chicken

Rabee,

Abdulameer,

Obed

et al. 2023

AAVS

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Background Antibiotic residues in poultry have the potential to have a number of harmful effects on health, including the emergence of multidrug-resistant microbial strains, allergic and an alteration of the normal flora in the intestines. The study aimed to evaluate the residue levels of enrofloxacin in chicken meat after administering berberine (BBR). Escherichia coli (E. coli) was isolated from chickens suffering from diarrhea, and its isolation was confirmed by culturing it on MacConkey agar medium. In this study, a total of one hundred chicken chicks were reared, and on the tenth day of the experiment, they were divided into five equal groups:NCG: This group represented the control and was given normal drinking water. T1: The chickens in this group were orally inoculated with E. coli at a dose of 0.5 ml containing 6x108 CFU/ml of E. coli. T2: The chickens in this group were orally inoculated with E. coli at a dose of 0.5 ml containing 6x108 CFU/ml of E. coli and were treated with enrofloxacin at a dose of 12.5 mg/kg orally through drinking water. T3 group: The chickens in this group were orally inoculated with E. coli at a dose of 0.5 ml containing 6x108 CFU/ml of E. coli and were treated with 250 mg/kg BBR through drinking water. T4 group: The chickens in this group were orally inoculated with E. coli at a dose of 0.5 ml containing 6x108 CFU/ml of E. coli and were treated with both combination enrofloxacin and BBR. Conclusion: study showed that berberine increased the residue levels of enrofloxacin in chicken meat, specifically in the thigh and liver. The docking study confirmed inhibitory activity of Cytochrome P450 3A4 (CYP3A4) and P-glycoprotein (P-gp) an enzyme responsible for the metabolism of antibiotics. Additionally, berberine demonstrated antibacterial effects against E. coli, as well as anti-inflammatory and antioxidant properties. Furthermore, histopathological examination of the intestinal broiler received BBR showed improvements and restore tissue near to normal.

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“…In addition, E. coli were isolated from fish found in coasts contaminated with sewage water (3). Also, E. coli was isolated from different animals such as cows and their environment (4) and from meat and the butchers' shop (5). Freshwater fish may contaminate with various types of pathogenic microorganisms from the various sources, which are usually either primary sources through the presence of these fishes in the aquatic environment or secondary sources during the harvesting of these fishes, as well as from transportation, marketing and storage, which lead to the pollution of these fishes and make them unsuitable for human consumption or to be carriers of some pathogens to humans (6).…”

Section: Introductionmentioning

confidence: 99%

Detection of some virulence gene Stx1, Stx2, and rfb of Escherichia coli isolated from fish in Nineveh governorate, Iraq

Alttai

Al-Sanjary

Sheet

2023

IJVS

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The aim of the current study is to detect the presence of some virulence genes of Escherichia (E.) coli in fish. A total of 46 strains of E. coli were previously isolated from 153 fish samples, including 28 and 18 isolate of E. coli from local markets and fish farms, respectively, in Nineveh Governorate from November 2021 to January 2022. The results of the study show that all isolates of E. coli possessed the uidA gene with a molecular weight of 623 bp. In addition, they show that 88.9% (16/18) isolates from farmed fish samples possessed the Stx1 gene with a molecular weight of 347 bp, while 72.2% (13/18) of them carried the Stx2 gene with a molecular weight of 589 bp. Also, the study unveils that 89.3 % (25/28) isolated from the market fish samples possessed the Stx1 gene with a molecular weight of 347 bp and 85.7% (24/28) isolates carried the Stx2 gene with a molecular weight of 589 bp. The rfb gene is not detected in this study, neither in farm fish nor in the samples from the local fish markets using the PCR technique. Likewise, it shows that E. coli isolated from fish possessed the Stx1 and Stx2 genes, which are major causative agents of food poisoning for consumers. Finally, the study gives important information about the application of health conditions on fish farms and fish markets to prevent contamination and reduce infections by foodborne pathogens that cause food poisoning for humans.

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Phenotypic and genotypic characterizations of Escherichia coli Isolated from veal meats and butchers’ shops in Mosul city, Iraq

Cited by 3 publications

References 26 publications

Contamination of Kazakhstan cheeses originating from Escherichia coli and its resistance to antimicrobial drugs

Contamination of Kazakhstan cheeses originating from Escherichia coli and its resistance to antimicrobial drugs

Effect of Berberine on Some Histopathological, Enrofloxacin Residue and Aniti-Diarrheaginic Escherichia coli in Broilar Chicken

Detection of some virulence gene Stx1, Stx2, and rfb of Escherichia coli isolated from fish in Nineveh governorate, Iraq

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