Phenotypic and genotypic characterizations of rhizobia isolated from root nodules of multiple legume species native of Fez, Morocco

Berrada, Halima; Nouioui, Imen; Houssaini, Mohammed Iraqui; Naiuml,; Ghachtouli, ma El; Gtari, Maher; Fikri-Benbrahim, Kawtar

doi:10.5897/ajmr11.1505

Cited by 23 publications

(8 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Increases in grain yields following inoculation of legumes with native rhizobia in this study are remarkable and disprove earlier reports by some authors that these crops do not respond to inoculation with rhizobia (Awonaike et al, 1990;de Freitas et al, 2012;Mweetwa et al, 2014). The results, however, agree with the findings of several authors including Bogino et al (2006), Boddey et al (2016), Ulzen et al (2016), and Martins et al (2003) that inoculating cowpea and groundnut leads to yield improvements.…”

Section: Discussionsupporting

confidence: 82%

Native Bradyrhizobium Strains From Ghana Can Enhance Grain Yields of Field-Grown Cowpea and Groundnut

et al. 2020

View full text Add to dashboard Cite

The existence of large population of ineffective native rhizobia and inconsistent performance of exotic strains in Ghanaian soils necessitate the need to identify effective and locally adapted elite strains for enhanced legume-rhizobium symbiosis. This study was designed to test the suitability of two previously selected potential elite Bradyrhizobium strains for use as inoculants on cowpea (Vigna unguiculata L. Walp) and groundnut (Arachis hypogaea L.) in multilocation experiments. Field experiments were set up at 26 locations (12 planted with cowpea and 14 planted with groundnut) in the Northern region of Ghana. Four treatments were applied at each location: inoculation with Bradyrhizobium strains KNUST 1002 and KNUST 1006, a positive nitrogen (+N) control and a negative control (without nitrogen or inoculation) arranged in randomized complete blocks with four replications. The results showed that inoculation with strains KNUST 1002 and KNUST 1006 promoted significant increases in grain yields of both cowpea and groundnut. On average, inoculating cowpea with strains KNUST 1002 and KNUST 1006 resulted in 63 and 52% increases in grain yield when compared to the negative control. Pod yields of groundnut, on the other hand, were significantly (p < 0.05) increased at 57% of the study locations with one or both test strains. Responses to inoculation were, however, highly variable across the different study locations (i.e., significant Treatment × Location Interaction, TLI). A detailed analysis of this significant TLI based on the genotype main effect (G) plus genotype-by-environment (GE) interaction (GGE) biplot analysis revealed that location contributed 71 and 88% of the variation observed in cowpea and groundnut, respectively, and grouped the locations into mega-environments for cowpea. These results demonstrate that native elite Bradyrhizobium strains KNUST 1002 and KNUST 1006 have potential for use as inoculants to increase cowpea and groundnut production in Northern Ghana.

show abstract

Section: Discussionsupporting

confidence: 82%

Native Bradyrhizobium Strains From Ghana Can Enhance Grain Yields of Field-Grown Cowpea and Groundnut

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The significant difference in the isolates' morphological characteristics could be an indicator of the existence of diverse native rhizobia-nodulating cowpea in the study locations. Isolates well adapted to the local environmental and climatic conditions with a high BNF could be considered for the development of commercial rhizobia inocula (Berrada et al, 2012).…”

Section: Morphological and Biochemical Classificationmentioning

confidence: 99%

Potential of Native Rhizobia to Improve Cowpea Growth and Production in Semiarid Regions of Kenya

Nyaga

Njeru

2020

Front. Agron.

View full text Add to dashboard Cite

Cowpea [Vigna unguiculata (L.) Walp] is an important crop for smallholder farmers in the marginal areas of sub-Saharan Africa. However, the crop growth and production are affected by low soil fertility due to poor soil management practices. Here, we assessed the effect of native and commercially available rhizobia inoculants on cowpea nodulation, growth, and yields on three local cowpea genotypes (K-80, M-66, and KVU 27-1) in the semiarid areas of Kenya. Field experiments were set in smallholder farms during the 2019 and 2020 cropping seasons. Native rhizobia were isolated from root nodules of cowpea plants used as trap cultures. The isolates were further assessed for symbiotic efficiency (SE) in the greenhouse and field experiments carried out during the short and long rain seasons. Field experiments were laid out in a randomized complete block design with three replications. The treatments consisted of the commercial inoculant (Biofix), native isolates, native + Biofix (consortium), and an uninoculated control. In the greenhouse, the native isolates significantly increased nodule number and dry weight (DW), shoot DW, and root DW when compared to the uninoculated control. Additionally, 50% of the isolates recorded SE of >80%, while 35.7 and 14.3% of the isolates had SE of 51–80 and <50%, respectively. In the field, rhizobia inoculation significantly (P < 0.05) increased nodulation and shoot DW compared to the uninoculated controls. Remarkably, rhizobia inoculation significantly increased yields where inoculation with native isolates recorded 22.7% increase in yield when compared to uninoculated control in the first season and 28.6% increase in yield in the second season. However, the rhizobia inoculants did not show a preference for any of the cowpea genotypes, and their performance was influenced by season and the study location. Our results demonstrate the existence of superior native isolates with potential to be developed to low-cost biofertilizer for sustainable cowpea production.

show abstract

“…Se lograron recuperar cinco aislamientos de Rhizobium spp., a partir de nódulos radiculares de plantas de frijol, en el Cuadro 3 se muestran los resultados a diferentes pruebas bioquímicas y las características de forma, borde, elevación, aspecto y color, las cuales nos indican que los aislamientos tienen las características de un buen inoculante del tipo de Rhizobium phaseoli, lo que concuerda con estudios realizados por (Cuadrado et al, 2009;Berrada et al, 2012;Granda et al, 2013).…”

Section: Resultados Y Discusiónunclassified

Incidencia y severidad del tizón común en plantas de frijol inoculados con Rhizobium phaseoli

Ramírez

Gallegos‐Morales

Castillo

et al. 2019

Remexca

View full text Add to dashboard Cite

El tizón común bacteriano Xanthomonas axonopodis pv. phaseoli es una enfermedad que ataca al cultivo de frijol (Phaseolus vulgaris), se encuentra presente 83% de las áreas de producción de semilla y hasta 79% en campos comerciales y reduce los rendimientos hasta 55%. La inoculación de Rhizobium en plantas como promotor de crecimiento vegetal, fijador de nitrógeno, a la síntesis del ácido indolacético, producen en la planta mayor vigor y tolerancia a las enfermedades. Se determinó el comportamiento de la incidencia y severidad del tizón común de plantas de frijol inoculadas con Rhizobium phaseoli y X. axonopodis pv. phaseoli. Para ello se aislaron cepas de Rhizobium (FM-1, N-2, PS-3, M-4, BJ-5) de nódulos radiculares de plantas de frijol y Xanthomonas (Xant 1) de manchas foliares necróticas. Los aislados se identificaron con base en características morfológicas celulares, coloniales y bioquímicas. Los ensayos en invernadero y en campo, se efectuaron en semillas inoculadas con 106 ufc mL-1 de Rhizobium y una segunda aplicación a los 10 días después de la siembra. Posteriormente 15 días después de la emergencia del frijol se inoculó por aspersión Xanthomonas axonopodis directamente a la planta. Transcurridos 30 días del cultivo en invernadero se observó que la severidad del tizón común fue inferior en plantas con presencia de nódulos en raíz (14.5%), que en el testigo (46%), también se encontró que las plantas inoculadas con Rhizobium phaseoli BJ-5 desarrollaron mayor vigor, diámetro de tallo, peso seco, número de hojas y longitud de raíz. En campo, la inoculación con la cepa BJ-5 mostró menor severidad (25.6%) respecto al testigo (55.8%) y resultó tener mejor crecimiento en la planta. La inoculación de frijol con Rhizobium phaseoli permite favorecer nutricional y fitosanitariamente al cultivo.

show abstract

Phenotypic and genotypic characterizations of rhizobia isolated from root nodules of multiple legume species native of Fez, Morocco

Cited by 23 publications

References 64 publications

Native Bradyrhizobium Strains From Ghana Can Enhance Grain Yields of Field-Grown Cowpea and Groundnut

Native Bradyrhizobium Strains From Ghana Can Enhance Grain Yields of Field-Grown Cowpea and Groundnut

Potential of Native Rhizobia to Improve Cowpea Growth and Production in Semiarid Regions of Kenya

Incidencia y severidad del tizón común en plantas de frijol inoculados con Rhizobium phaseoli

Contact Info

Product

Resources

About