Phenotypic and genotypic comparison of ESBL production by Vaginal Escherichia coli isolates from pregnant and non-pregnant women

Al-Mayahie, Sareaa Maseer Gatya

doi:10.1186/1476-0711-12-7

Cited by 37 publications

(24 citation statements)

References 26 publications

(35 reference statements)

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“…The production of these enzymes in strains from pregnant patients has been reported by other researchers such as Ramos in 2012 and Al-Mayahie in 2013, but these authors report the phenotype in lower percentage than what we documented. 45,46 Interestingly, in the present study, the percentage of ESBL phenotype was lower than the percentage of strains resistant to β-lactams, this could indicate that UPEC strains could present other mechanisms of resistance to this family of antibiotics, as the production of an altered penicillin binding proteins (PBP) which decrease the affinity of the antibiotic for PBP or efflux pumps that lead to bacterial survival and therapeutic failure. 47,48 The predominant virulence factors present in pregnant and non-pregnant women of both states were those involved in adhesion and iron acquisition.…”

Section: Discussioncontrasting

confidence: 49%

Virulence and Resistance Determinants of Uropathogenic Escherichia coli Strains Isolated from Pregnant and Non-Pregnant Women from Two States in Mexico

Ballesteros-Monrreal

Arenas-Hernández

Enciso‐Martínez

et al. 2020

IDR

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Background/Purpose: Uropathogenic E. coli (UPEC) is the main cause of urinary tract infection (UTI) and it is known that pregnant women have a higher risk for UTI. UPEC has a variety of virulence and antibiotic resistance factors that facilitate its pathogenic success and it is crucial to know which are the susceptibility patterns, Extended-Spectrum-β-Lactamase (ESBL) production, virulence genes, pathogenicity islands (PAI), phylogenetic groups and serotypes among strains isolated from pregnant and non-pregnant women. Methods: One hundred fifty UPEC strains were isolated from pregnant and non-pregnant women from two different Mexican states (Sonora and Puebla). Strains were analyzed using the Kirby-Bauer method for the determination of antibiotic susceptibility and ESBL. Virulence genes, PAIs and phylogenetic groups were determined using a multiplex PCR. Strains were serotyped by an agglutination assay. Blood agar and CAS agar were used for phenotypic assays. Results: 92.7% of UPEC strains showed multidrug-resistant (MDR), 6.7% extremely-resistant (XDR) and 0.6% pandrug-resistant (PDR). The highest resistance was determined to be for βlactam antibiotics (>72% in both states) and 44.5% of the UPEC strains were ESBL + . The predominant virulence genes found were fimH (100%), iucD (85%) and iha (60%). The strains isolated from pregnant women from Puebla presented a large percentage of genes associated with upper urinary tract infections. PAIs were found in 51% and 68% of the strains from Sonora and Puebla, respectively. All the strains were siderophores producers and 41.5% produced hemolysis. The serotypes found were diverse and belonged to phylogroups A, B2 and C. Conclusion: The UPEC strains from this study are MDR with tendency to XDR or PDR, they can cause upper UTIs and are serotypically and phylogenetically diverse, which supports the need to develop new strategies for UTI treatment in pregnant and nonpregnant Mexican women.

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Section: Discussioncontrasting

confidence: 49%

Virulence and Resistance Determinants of Uropathogenic Escherichia coli Strains Isolated from Pregnant and Non-Pregnant Women from Two States in Mexico

Ballesteros-Monrreal

Arenas-Hernández

Enciso‐Martínez

et al. 2020

IDR

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“…Species by Multiplex PCR Analysis. All confirmed E. coli and Klebsiella isolates were screened for the presence of the blaCTX-M, blaOXA, blaSHV, blaTEM, blaCMY, blaCMY-1, and blaCMY-2 ESBL producing genes determinants using previously described PCR protocol [26,27]. e primer sequences, targeted genes, amplicon sizes, and PCR conditions are listed in Table 2.…”

Section: Detection Of Esbl Genes In E Coli and Klebsiellamentioning

confidence: 99%

Antimicrobial Resistance Factors of Extended-Spectrum Beta-Lactamases Producing Escherichia coli and Klebsiella pneumoniae Isolated from Cattle Farms and Raw Beef in North-West Province, South Africa

Montso

Dlamini

Kumar

et al. 2019

BioMed Research International

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Background. Extended spectrum beta-lactamases (ESBLs) producing Enterobacteriaceae cause severe infections in humans which leads to complicated diseases. There is increasing evidence that cattle contribute to the development and spread of multidrug resistant pathogens and this raises public health concern. Despite this, data on the concurrence of ESBL producing pathogens in cattle, especially in the North-West province are rare. Therefore, the aim of the present study was to isolate, identify and characterise ESBL producing E. coli and K. pneumoniae species from cattle faeces and raw beef samples. Results. A total of 151 samples comprising 55 faeces samples and 96 raw beef samples were collected and 259 nonreplicative potential isolates of Enterobacteriaceae were obtained. One hundred and ninety-six isolates were confirmed as E. coli (114; 44%) and K. pneumoniae (82; 32%) species through amplification of uspA and uidA and ntrA gene fragments, respectively. Antimicrobial susceptibility test revealed that large proportions (66.7–100%) of the isolates were resistant to Amoxicillin, Aztreonam, Ceftazidime, Cefotaxime, and Piperacillin and were multidrug resistant isolates. Cluster analysis of antibiotic inhibition zone diameter data revealed close similarities between isolates from different sources or species thus suggested a link in antibiotic exposures. The isolates showing phenotypic resistance against ESBL antimicrobial susceptibility tests were screened for the presence of ESBL gene determinants. It was observed that 53.1% of the isolates harboured ESBL gene determinants. The blaTEM, blaSHV and blaCTX-M genes were detected in E. coli isolates (85.5%, 69.6%, and 58%, respectively) while blaCTX-M and blaOXA were detected in K. pneumoniae (40% and 42.9%, respectively). All the genetically confirmed ESBL producing E. coli and K. pneumoniae isolates were subjected to Enterobacterial Repetitive Intergenic Consensus (ERIC) PCR analysis. Fingerprinting data revealed great similarities between isolates from different areas and sources which indicates cross-contamination between cattle and beef. Conclusion. This study revealed that cattle and its associated food products, beef in particular, harbour ESBL producing pathogens. And this warrants a need to enforce hygiene measures and to develop other mitigation strategies to minimise the spread of antibiotic resistant pathogens from animals to human.

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“…Screening for ESBL genes, TEM, SHV, and CTX-M, was done by a conventional, multiplex, polymerase chain reaction (PCR) assay with PCR master mix (DreamTaq Green PCR Master Mix; Thermo Scientific) using specific primers (Table 1). [31][32][33][34] The cycling parameters were as follows: initial denaturation at 94°C for 5 min, followed by 35 cycles at 94°C for 30 sec, 45°C for 1 min, and 72°C for 1 min, and a final extension at 72°C for 10 min. The amplified PCR products were subjected to electrophoresis on a 1.5% agarose gel in 0.5 • TBE buffer.…”

Section: Molecular Detection Of B-lactamase Genesmentioning

confidence: 99%

Phenotypic and Genotypic Characterization of Extended-Spectrum β-Lactamase-Producing Enterobacteriaceae in Asymptomatic Bacteriuria in Pregnancy

Youssef

Rizk

Hassuna

2019

Microbial Drug Resistance

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Asymptomatic bacteriuria (ASB) has been consistently observed in pregnancy. However, there is a paucity of data on the prevalence and characteristics of extended-spectrum b-lactamase (ESBL)-producing Enterobacteriaceae in ASB in pregnant women. Therefore, we sought to investigate ESBL-producing and multidrug-resistant Enterobacteriaceae in antenatal women with ASB. Urine samples were collected from 310 asymptomatic pregnant women attending primary antenatal clinics and screened for significant bacteriuria. Isolates of Enterobacteriaceae were phenotypically tested for their ESBL production. ESBL genes (CTX-M, TEM, and SHV genes) were then amplified by polymerase chain reaction (PCR). Multiplex PCRs were used to perform phylogenetic typing of ESBLproducing Escherichia coli isolates and to examine the commonality of sequence type 131 (ST131)-O25b and ST131-O16. A total of 103 (33.2%) pregnant women were positive for significant bacteriuria (80 Enterobacteriaceae). Of these isolates, 32.5% (n = 26) were ESBL producers and had a higher rate of multidrug resistance than non-ESBL producers. Genotypic characterization of ESBL-producing isolates showed that 84.6% had the bla CTX-M gene (bla CTX-M-15 = 77.3%; bla CTX-M-9 = 18.2%). None of the isolates were of the TEM or SHV type. Half of the ESBLproducing E. coli isolates were of the phylogroup B2, and 4 (20%) isolates were of the ST131-O16 clonal subgroup. This study is the first in Egypt to provide evidence for the high prevalence of ESBL-producing Enterobacteriaceae in pregnant women with ASB. It also represents an important step toward genotypic characterization of this resistant form of bacteria, which may be useful for future antimicrobial studies.

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Phenotypic and genotypic comparison of ESBL production by Vaginal Escherichia coli isolates from pregnant and non-pregnant women

Cited by 37 publications

References 26 publications

<p>Virulence and Resistance Determinants of Uropathogenic <em>Escherichia coli</em> Strains Isolated from Pregnant and Non-Pregnant Women from Two States in Mexico</p>

<p>Virulence and Resistance Determinants of Uropathogenic <em>Escherichia coli</em> Strains Isolated from Pregnant and Non-Pregnant Women from Two States in Mexico</p>

Antimicrobial Resistance Factors of Extended-Spectrum Beta-Lactamases Producing Escherichia coli and Klebsiella pneumoniae Isolated from Cattle Farms and Raw Beef in North-West Province, South Africa

Phenotypic and Genotypic Characterization of Extended-Spectrum β-Lactamase-Producing Enterobacteriaceae in Asymptomatic Bacteriuria in Pregnancy

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