Phenotypic and genotypic study on antimicrobial resistance patterns of E. coli isolates from bovine mastitis

Fazel, Fatemeh; Jamshidi, Abdollah; Khoramian, Babak

doi:10.1016/j.micpath.2019.05.018

Cited by 32 publications

(21 citation statements)

References 17 publications

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“…In line with previous reports,5 6 10 42 the isolates from the present study were mainly of phylogenetic groups A and B1, with the exception of isolates belonging to phylogroup C, which were not identified in earlier studies that restricted classifications to four main phylogenetic groups A, B1, B2 and D 43. Phylogroup C is closely related to group B1, but before its recognition as a distinct phylogroup its members were classified within group A 44.…”

Section: Discussionsupporting

confidence: 91%

Molecular types, virulence profiles and antimicrobial resistance of Escherichia coli causing bovine mastitis

Nüesch‐Inderbinen

Käppeli

Morach

et al. 2019

Veterinary Record Open

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BackgroundEscherichia coli is an important aetiological agent of bovine mastitis worldwide.MethodsIn this study, 82 E. coli from bovine mastitis milk samples from 49 farms were analysed for their genetic diversity using phylogenetic grouping and multilocus sequence typing. The isolates were examined by PCR for a selection of virulence factors (VFs). Antimicrobial susceptibility profiles were assessed using the disk diffusion method.ResultsThe most prevalent phylogroups were group B1 (41.5 per cent of the isolates) and group A (30.5 per cent). A variety of 35 different sequence types (STs) were identified, including ST1125 (11 per cent), ST58 (9.8 per cent), ST10 (8.5 per cent) and ST88 (7.3 per cent). Aggregate VF scores (the number of unique VFs detected for each isolate) ranged from 1 to 3 for 63.4 per cent of the isolates and were at least 4 for 12.2 per cent. For 24.4 per cent of the isolates, the score was 0. The three most frequent VFs were traT, fyuA and iutA. The majority (72 per cent) of the isolates harboured traT. The majority (68.3 per cent) of the isolates were fully susceptible to all antimicrobials tested, with 22 per cent resistant to ampicillin and 14.6 per cent to tetracycline. Resistance rates were low for gentamicin (3.7 per cent), amoxicillin/clavulanic acid (2.4 per cent) and ceftiofur (1.2 per cent), respectively.ConclusionAmong the study’s sample population, E. coli strains were genotypically diverse, even in cows from the same farm, although some STs occurred more frequently than others. Susceptibility to clinically relevant compounds remained high.

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Section: Discussionsupporting

confidence: 91%

Molecular types, virulence profiles and antimicrobial resistance of Escherichia coli causing bovine mastitis

Nüesch‐Inderbinen

Käppeli

Morach

et al. 2019

Veterinary Record Open

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“…sul3 -integrons are widely distributed within commensal and pathogenic Enterobacteriaceae from humans [ 54 ], a range of food animals including cattle [ 55 ], swine [ 8 ] and poultry [ 56 ] and white stork [ 46 ]. The sul3 gene was reported not only from specimens collected in animal farms, but also from surface sediment in lakes where Cu and Zn resistance genes [ 57 ] were present, and wetlands constructed to treat pig manure [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

Genomic Characterisation of a Multiple Drug Resistant IncHI2 ST4 Plasmid in Escherichia coli ST744 in Australia

et al. 2020

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Antibiotic resistance genes (ARGs) including those from the blaCTX-M family and mcr-1 that encode resistance to extended spectrum β–lactams and colistin, respectively, have been linked with IncHI2 plasmids isolated from swine production facilities globally but not in IncHI2 plasmids from Australia. Here we describe the first complete sequence of a multiple drug resistance Australian IncHI2-ST4 plasmid, pTZ41_1P, from a commensal E. coli from a healthy piglet. pTZ41_1P carries genes conferring resistance to heavy-metals (copper, silver, tellurium and arsenic), β-lactams, aminoglycosides and sulphonamides. The ARGs reside within a complex resistance locus (CRL) that shows considerable sequence identity to a CRL in pSDE_SvHI2, an IncHI2:ST3 plasmid from an enterotoxigenic E. coli with serotype O157:H19 of porcine origin that caused substantial losses to swine production operations in Australia in 2007. pTZ41_1P is closely related to IncHI2 plasmids found in E. coli and Salmonella enterica from porcine, avian and human sources in Europe and China but it does not carry genes encoding resistance to clinically-important antibiotics. We identified regions of IncHI2 plasmids that contribute to the genetic plasticity of this group of plasmids and highlight how they may readily acquire new resistance gene cargo. Genomic surveillance should be improved to monitor IncHI2 plasmids.

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“…Sulfonamineresistant genes (sul) have also spread widely for these reason. Analogously, with regard to the detection rate of sul, we compared several recent studies [23][24][25][26] found that the detection rate of sulfonamineresistant genes was high and that the detection rate of sul1 and sul2 in sulfamine-resistant genes was generally higher than that of sul3. It was suggested that there may be more sulfamine-resistant bacteria in Nanning, and we should pay more attention to them.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial and Transconjugants Characteristics of Sul3 Positive Escherichia Coli Isolated from Animals in Nanning, Guangxi Province

Chen

Zhang

et al. 2020

Preprint

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Background: Sulfonamides is the second most popular antibiotic in many countries, which leads to the widespread emergence of sulfonamides resistance. Sul3 is a late sulfanilamide resistance gene, whose research is relatively little. Result: 46 sul3 positive E. coli strains were separated. A total of 12 ST types were observed, and 1 of those was previously unknown type. The ST350 is the most numerous type. All isolates were multidrug-resistant E. coli, with high antimicrobial rates to penicillin, ceftriaxone sodium, streptomycin, tetracycline, ciprofloxacin, gatifloxacin and chloramphenicol (100%, 73.9%, 82.6%, 100%, 80.4%, 71.7% and 97.8%), and with at least 3 resistance genes in addition to sul3. The plasmids transfered from 3 sul3-positive isolates to C600, the most of which brought 7 antibiotic resistance and increased resistance genes to C600. The transferred sul3 gene and the plasmid that carries it could be stably inherited in the recipient bacteria for at least 20 days. Those plasmids had no effect on the growth of the recipient bacteria, but it would greatly reduce (at least 60 time) the in vitro competitiveness of the strains. Conclusions: In Nanning, these sul3-positive Escherichia coli have strong antimicrobial resistance, and the plasmid carrying sul3 has the ability to transfer multiple resistance genes, so long-term monitoring is necessary. Since the transferred plasmid will greatly reduce the in vitro competitiveness of the strain, we can consider limiting the spread of antimicrobial in this respect.

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Phenotypic and genotypic study on antimicrobial resistance patterns of E. coli isolates from bovine mastitis

Cited by 32 publications

References 17 publications

Molecular types, virulence profiles and antimicrobial resistance of Escherichia coli causing bovine mastitis

Molecular types, virulence profiles and antimicrobial resistance of Escherichia coli causing bovine mastitis

Genomic Characterisation of a Multiple Drug Resistant IncHI2 ST4 Plasmid in Escherichia coli ST744 in Australia

Antimicrobial and Transconjugants Characteristics of Sul3 Positive Escherichia Coli Isolated from Animals in Nanning, Guangxi Province

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