Phenotypic and molecular analysis of dominant occurring antibiotic active-producing Streptomyces soil flora in Northern Jordan

Abstract

This investigation aimed to determine the relatedness of dominant occurring soil Streptomyces spp. in Northern Jordan based on their RAPD-PCR fingerprints, and to compare RAPD technique with the conventional phenotypic characterization of Streptomyces isolates. Fifty-eight white and gray color-bearing aerial mycelia antibiotic active-producing Streptomyces soil isolates along with three reference strains were genetically analyzed by RAPD-PCR. Polymorphisms between the isolates showed 1 to 10 bands per isolate and ranged from 200 to 3200 bp in size. Results revealed one common band of ~600 bp shared by ~85% of the isolates, and the observation of bands specific to some reference strains and some soil isolates. When RAPD patterns were analyzed with the UPGMA, results revealed clustering the tested isolates into two equal main super clusters (50% each). Super cluster I appeared to be homogenous and include the three reference strains. However, super cluster II was heterogeneous and but not including any of the reference strains. The association of the antibiotic activity of the dominant white and gray aerial mycelium-bearing Streptomyces isolates to RAPD clustering is reported for the first time, and the RAPD-PCR fingerprints generated here deserve to be cloned, characterized and sequenced in future as Streptomyces species-specific DNA markers. The more random primers used in the analysis may add to RAPD technique a cost-effective, fast, precise result, and less labor work solution for analyzing the similarities and differences among the Streptomyces isolates.

Biological control and plant growth promotion properties of Streptomyces albidoflavus St-220 isolated from Salvia miltiorrhiza rhizosphere

“…The strains of Streptomyces genus employ their secondary metabolites as weapons to inhibit phytopathogenic fungi ( Amin et al, 2021 ; Hotta, 2021 ; Mahasneh et al, 2021 ; Terra et al, 2021 ). In this study, genome sequencing revealed that the chromosome of the St. albidoflavus St-220 contained 21 conserved biosynthesis gene clusters (BGCs), of which 10 showed high similarities in structure with known BGCs encoding terpenes, non-ribosomal peptides, polyketides, siderophores, and ectoines, which had been proven to participate in the regulation of antimicrobial activities of Streptomyces strains ( van Bergeijk et al, 2020 ).…”

Molecular identification, antibacterial activity, and production of hydrolytic enzymes by halotolerant bacterium Streptomyces sp. ESM2-25 GTF strain isolated from extreme environment in north-east of Algeria

“…In fact, these microorganisms produce 45 % of the bioactive molecules (Berdy 2005). Among the actinomycetes, the genus Streptomyces is the most important producer of bioactive molecules (Kieser et al 2000;Hopwood 2007a; Kovácsová et al 2017;Mahasneh et al 2021) including antibacterial agents such as tetracyclines, antifungal agents such as amphotericin B, ancticancer drugs exemplified by adriamycin, the immunosuppressant tacrolimus, enzymes such as amylase, lipase, protease, cellulase, and chitinase (Miyadoh 1993;Hopwood 2007b;Suthindhiran and Kannabiran 2009;Kumar et al 2020). Streptomyces species have been reported to produce nearly 80 % of the antibiotics known currently (Miyadoh 1993;Kieser et al 2000).…”