Phenotypic and molecular characterization of chickpea rhizobia isolated from different areas of Morocco

Maâtallah, Jamila; Berraho, El Bekkay; Muñoz, Socorro; Sanjuán, Juan; Lluch, Carmen

doi:10.1046/j.1365-2672.2002.01718.x

Cited by 94 publications

(86 citation statements)

References 38 publications

(99 reference statements)

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“…The sequence of the nifH gene showed 99.6% similarity with several strains of Mesorhizobium ciceri reported to nodulate chickpea in Africa (25). These results differ from those obtained previously based on nifD sequences from Bradyrhizobium strains that showed a great degree of local divergence (36); nevertheless, additional studies including other rhizobial species from various geographical locations should be performed to establish definitive conclusions about the relation between the phylogeny of nif genes and the geographical origin of strains carrying these genes.…”

Section: Nodulation Of Lupinus the Fast-growing Isolates Lup21contrasting

confidence: 70%

Nodulation of Lupinus albus by Strains of Ochrobactrum lupini sp. nov

Trujillo¹,

Willems

Abril

et al. 2005

Appl Environ Microbiol

211

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The nodulation of legumes has for more than a century been considered an exclusive capacity of a group of microorganisms commonly known as rhizobia and belonging to the ␣-Proteobacteria. However, in the last 3 years four nonrhizobial species, belonging to ␣ and ␤ subclasses of the Proteobacteria, have been described as legume-nodulating bacteria. In the present study, two fast-growing strains, LUP21 and LUP23, were isolated from nodules of Lupinus honoratus. The phylogenetic analysis based on the 16S and 23S rRNA gene sequences showed that the isolates belong to the genus Ochrobactrum. The strains were able to reinfect Lupinus plants. A plasmid profile analysis showed the presence of three plasmids. The nodD and nifH genes were located on these plasmids, and their sequences were obtained. These sequences showed a close resemblance to the nodD and nifH genes of rhizobial species, suggesting that the nodD and nifH genes carried by strain LUP21 T were acquired by horizontal gene transfer. A polyphasic study including phenotypic, chemotaxonomic, and molecular features of the strains isolated in this study showed that they belong to a new species of the genus Ochrobactrum for which we propose the name Ochrobactrum lupini sp. nov. Strain LUP21 T (LMG 20667 T ) is the type strain.Plants from the family Leguminosae are usually capable of dinitrogen fixation because of their symbiotic interaction with nodulating bacteria belonging to the order Rhizobiales. Most bacteria that establish a symbiosis with legume plants, including some nonrhizobial species of Methylobacterium (16, 45) and Devosia (40, 41), belong to the ␣ subclass of Proteobacteria, although some species from genera of the ␤ subclass, such as Ralstonia and Burkholderia, can also nodulate legumes (6,32,53). In the last few years there has been an increasing amount of research focused on bacteria that nodulate stems or roots of tropical legume species. However, the identity of many of the endosymbionts of temperate legumes still remains unknown. The genus Lupinus groups up to 200 species of herbs and small shrubs, broadly distributed in the Mediterranean area and in the American continent, where they colonize very different environments. Despite the agronomic and ecological interest of Lupinus, this plant has been poorly studied with respect to its symbionts. Plants from the genus Lupinus are nodulated by fast-and slow-growing rhizobia; however, slow-growing rhizobia are more frequently isolated from this legume (3,5,21,30). The data obtained from the small-subunit (SSU) rRNA gene indicate a very close relationship between some bradyrhizobia isolated from Lupinus and Bradyrhizobium japonicum (3,12,30). However, bacterial strains nodulating Lupinus plants have been poorly characterized thus far, and fast-growing species nodulating this legume have not been not officially described; nevertheless, in the past the species Rhizobium lupini was proposed (15) and was later abandoned (14).During a study of rhizobia nodulating Lupinus plants in several geographical regi...

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Section: Nodulation Of Lupinus the Fast-growing Isolates Lup21contrasting

confidence: 70%

Nodulation of Lupinus albus by Strains of Ochrobactrum lupini sp. nov

Trujillo¹,

Willems

Abril

et al. 2005

Appl Environ Microbiol

211

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show abstract

“…Alexandre et al, (2009) reported that temperature greatly affected the growth and symbiotic performance of Rhizobia. Maatallah et al, (2002) To observe pH tolerance of the rhizobial isolates, they were grown in YEMA medium by adjusting pH to 5-9 and the plates were incubated at 28 0 C for 36 hours. In the present study, optimum pH for rhizobial growth was between 6-7.…”

Section: Effect Of Salt Ph and Temperature On Rhizobium Growthmentioning

confidence: 99%

Isolation and Characterization of Rhizobium from Chickpea (Cicer arietinum)

Wadhwa¹,

Srivastava²,

Rani³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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“…The sequences of the oligonucleotide primers used for amplification of 16S rRNA genes are: Forward primer 41f (5'-GCTCAAGATTGAACGCTGGCG-3'); Reverse primer 1488r (5'-GTTACCTTGTTACGACTTCACC-3') (Maatallah et al, 2002). The stock solution (100 ng ml -1 ) of primers was prepared by reconstituting lyophilized primers in sterilized deionized (milliQ) water and stored at 20°C.…”

Section: Primersmentioning

confidence: 99%