Phenotypic and molecular characterization of chrysanthemum white rust pathogen Puccinia horiana (Henn) and the effect of liquid based formulation of Bacillus spp. for the management of chrysanthemum white rust under protected cultivation

Dheepa, R.; Vinodkumar, S.; Renukadevi, P.; Nakkeeran, S.

doi:10.1016/j.biocontrol.2016.09.006

Cited by 28 publications

(13 citation statements)

References 26 publications

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“…Crude metabolites of the two most effective strains among the 55 dually challenged strains were extracted with ethyl acetate (Dheepa et al, 2016). The resulting condensate (100 mg) was dissolved in 1 ml of HPLC-grade methanol.…”

Section: Methodsmentioning

confidence: 99%

“…Crude metabolites of the two most effective strains of B. amyloliquefaciens VB2 and VB7 were analyzed by GC-MS (Trace GC Ultra DSQ II, Thermo Scientific, Germany) for the detection of active biomolecules responsible for the suppression of S. sclerotiorum , as proposed by Dheepa et al (2016). …”

Section: Methodsmentioning

confidence: 99%

“…Subsequently, cfu were assessed in nutrient agar medium using the serial dilution technique. The bacterial suspension was prepared comprising minimum of 2.5 × 10 10 cfu/ml (Dheepa et al, 2016). …”

Section: Methodsmentioning

confidence: 99%

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Biocontrol Potentials of Antimicrobial Peptide Producing Bacillus Species: Multifaceted Antagonists for the Management of Stem Rot of Carnation Caused by Sclerotinia sclerotiorum

et al. 2017

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Bacillus species are widely exploited as biocontrol agents because of their efficiency in impeding various plant pathogens with multifaceted approach. In this study, Bacillus species were isolated from rhizosphere of various plants viz., carnations, cotton, turmeric, and bananas in Tamil Nadu state of India. Their potential to control the mycelial growth of Sclerotinia sclerotiorum was assessed in vitro by dual plate and partition plate techniques. B. amyloliquefaciens strain VB7 was much effective in inhibiting mycelial growth (45% inhibition of over control) and sclerotial production (100%). PCR detection of AMP genes revealed that B. amyloliquefaciens (VB7) had a maximum of 10 diverse antibiotic biosynthesis genes, namely, ituD, ipa14, bacA, bacD, bamC, sfP, spaC, spaS, alba, and albF, that resulted in production of the antibiotics iturin, bacilysin, bacillomycin, surfactin, subtilin, and subtilosin. Further, metabolites from B. amyloliquefaciens strains VB2 and VB7, associated with inhibition of S. sclerotiorum, were identified as phenols and fatty acids by gas chromatography mass spectrometry (GC-MS). Delivery of bacterial suspension of the effective strains of Bacillus spp. as root dip was found promising for the management of stem rot of cultivated carnations. Minimal percent disease incidence (4.6%) and maximum plant growth promotion was observed in the plants treated with B. amyloliquefaciens (VB7).

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Biocontrol Potentials of Antimicrobial Peptide Producing Bacillus Species: Multifaceted Antagonists for the Management of Stem Rot of Carnation Caused by Sclerotinia sclerotiorum

et al. 2017

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“…The solvent fraction with antifungal compounds were combined and concentrated by evaporation in the rotary flask evaporator maintained at 60°C at 80 rpm. The concentrated crude metabolites of the extracellular antifungal compounds obtained from the culture broth were dissolved in 1 ml HPLC grade methanol and used for GC/MS analysis (Dheepa et al, 2016).…”

Section: Extraction and Detection Of Nonvolatile Metabolitesmentioning

confidence: 99%

Antifungal activity of Bacillus subtilis subsp. spizizenii (MM19) for the management of Alternaria leaf blight of marigold

Priyanka

Nakkeeran

Pravin

et al. 2018

Journal of Biological Control

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“…penyakit karat putih adalah satu kendala budidaya yang hingga saat ini masih menyebabkan rendahnya kuantitas dan harga jual produk (Hanudin & Marwoto 2012 Dheepa et al 2016). Dalam skala produksi, kerugian yang diakibatkan cendawan ini dapat mencapai 80% (Yusuf et al 2017) dan pada intensitas tinggi dapat menyebabkan kegagalan panen (Hanudin, Marwoto & Djatnika 2015).…”

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Pengaruh Formulasi Biofungsida Berbahan Aktif Cladosporium sp. Terhadap Penyakit Karat Putih Pada Tanaman Krisan (Effect of Biofungcide Formulation Based on Cladosporium sp. Against White Rust Disease on Chrysanthemum)

Yusuf¹,

Nuryani²,

Budiarto³

2019

J.Hort

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Pengendalian penyakit karat putih pada tanaman krisan dengan menggunakan agens hayati merupakan salah satu upaya penting dalam mewujudkan sistem pertanian yang berkelanjutan dan ramah lingkungan. Penelitian bertujuan mengevaluasi kemangkusan biofungisida berbahan aktif Cladosporium sp. pada beberapa formulasi (WP-1, WP-2, dan WP-3) dibandingkan dengan penggunaan fungisida sintetik dan asam salisilat dalam mengendalikan penyakit karat pada krisan cv. Puspita Nusantara. Penelitian berlangsung selama 1 tahun dari bulan Januari hingga Desember 2016, dilakukan melalui dua tahap, yaitu penelitian super impose di Kebun Percobaan Balai Penelitian Tanaman Hias dengan menggunakan rancangan acak lengkap terdiri atas enam perlakuan (WP-1, WP-2, WP-3, AS, FS, dan kontrol) dengan lima ulangan, setiap ulangan terdiri atas 100 tanaman. Aplikasi dilakukan setiap minggu pada sore hari dan verifikasi kemangkusan di lahan petani menggunakan uji t berpasangan terdiri atas dua perlakuan (WP-1 2 g/l dan fungisida sintetik berbahan aktif pyraclostrobin 250 g/l) dengan 10 ulangan. Hasil evaluasi kemangkusan perlakuan biofungisida menunjukkan bahwa seluruh formulasi mampu menekan serangan penyakit karat sebesar 17% hingga 27%. Demikian juga dengan hasil uji di lahan petani formulasi WP-1 mampu mengimbangi fungisida sintetik dengan menyisakan serangan karat 1,6% dan 1,7%. Hasil percobaan menunjukkan bahwa seluruh formulasi biofungisida mempunyai keefektifan yang sama dalam menurunkan intensitas dan perkembangan penyakit karat, dengan fungisida sintetik berbahan aktif pyraclostrobin 250 g/l dengan kualitas bunga yang cenderung lebih baik dibandingkan dengan kultur teknis petani yang berbasis fungisida sintetik.KeywordsDendranthema grandiflora; Agens hayati; Intensitas serangan; Evaluasi lapanganAbstract The application of biological agents to control white rust on chrysanthemum is one important issues in the era of ecofriendly and sustainable agriculture, including in chrysanthemum production system. The research was carried out to evaluate the application of several biofungicide formulations with the active ingredient of Cladosporium sp., i.e. WP-1, WP-2, and WP-3, synthetic fungicide and salicylic acid for white rust control in chrysanthemum cv. Puspita Nusantara. The research was conducted from January to December 2015, through two subsequent experiments, i.e. super-impose experiment that located in Indonesian Ornamental Crops Research Institution using a complete randomized design consisting of six treatments (WP-1, WP-2, WP-3, AS, FS, and control) and five replications and verification of biofungicide effectivess at the growers’ nursery using paired t test consisting of dua treatments (WP-1 2 g/l) dan synthetic fungicide with active ingridient pyraclostrobin 250 g/l) and 10 replications. The results of evaluation of biofungicide treatment efficacy showed that all of formulations biofungicide formulation were able to suppress rust attack by 17% to 27%. Likewise with the results of tests on farmer land the WP-1 formulation was able to compensate for synthetic fungicide with 1.6% and 1.7% rust attack. The results of this experiment showed that all of biofungicide formulations and synthetic fungicides had the same effectiveness in reducing the intensity and development of rust disease and improved flower quality compared to farmers’ technical culture based on synthetic fungicides.

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Phenotypic and molecular characterization of chrysanthemum white rust pathogen Puccinia horiana (Henn) and the effect of liquid based formulation of Bacillus spp. for the management of chrysanthemum white rust under protected cultivation

Cited by 28 publications

References 26 publications

Biocontrol Potentials of Antimicrobial Peptide Producing Bacillus Species: Multifaceted Antagonists for the Management of Stem Rot of Carnation Caused by Sclerotinia sclerotiorum

Biocontrol Potentials of Antimicrobial Peptide Producing Bacillus Species: Multifaceted Antagonists for the Management of Stem Rot of Carnation Caused by Sclerotinia sclerotiorum

Antifungal activity of Bacillus subtilis subsp. spizizenii (MM19) for the management of Alternaria leaf blight of marigold

Pengaruh Formulasi Biofungsida Berbahan Aktif Cladosporium sp. Terhadap Penyakit Karat Putih Pada Tanaman Krisan (Effect of Biofungcide Formulation Based on Cladosporium sp. Against White Rust Disease on Chrysanthemum)

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