Phenotypic and Molecular Detection of Metallo-β –Lactamase Producing Pseudomonas aeruginosa Isolates From Different Clinical Infections in Erbil

Abstract

Metallo-β-lactamase (MBL) producing Pseudomonas aeruginosa has been documented to be a critical nosocomial infection. Itwas continuous intrinsic and acquired resistance to a various group of antimicrobial agents and its resistance ability to develop multidrug resistance lead to a severe therapeutic problem. The study aimed to identify the molecular characterisation of clinical isolates of Metallo-β-lactamase P. aeruginosain Erbil hospitals. This study was carried out during the period from October 2017 to March 2018. A total of 300 clinical specimens were collected from patients (urine 124, wound 80, burns 40, bronchial wash 30, and sputum 26) aged 15-65 years attending Rizgary, West emergency, Erbil teaching hospitals. Out of 300 specimens, 50 isolates of P. aeruginosawere recovered and accounted for 16% of hospitalised infection isolates, the diagnosis of P. aeruginosaisolates was confirmed phenotypically and genotypically via the amplification of 16SrRNAgeneby using PCR technique. All isolates were tested toward the different class of antimicrobials by using agar diffusion

Antibiotic Sensitivity and Biofilm Formation in Pseudomonas aeruginosa Isolated from Wound Infections in Baghdad City

“…P. aeruginosa isolates were highly susceptible (low resistance) to meropenem 13/30 (43.3%) and imipenem 9/30 (30%) (see Table 3). The findings of this study demonstrated that all P. aeruginosa isolates were completely resistant 100% to piperacillin and it was consistent with the findings from a previous investigation in Irbil, Iraq, found that all isolates of P. aeruginosa were piperacillin-resistant 100% [32]. According to the data from earlier studies found different results from ours, which were (32% and 37%), respectively [33,34].…”