Phenotypic and molecular genetic properties of Escherichia coli clinical strains isolated from patients with urological  diseases

Slukin, Pavel V.; Svetoch, E. A.; Aslanyan, E M; Асташкин, Е. И.; Ершова, М Г; Poletaeva, E D; Шепелин, А П; Fursova, Nadezhda K.

doi:10.18565/urology.2020.2.23-30

Cited by 2 publications

(1 citation statement)

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“…Moreover, the absence of cross-resistance was shown for HfBRI-25 when testing in vitro against selected PmxB-resistant E. coli MDR CI 1057 (Table 2, Figure 5). Interestingly, there were two strains with the MCR-1-mediated colistin resistance among bacteria sensitive to HfBRI-25 [32]. MCR-1 is a member of the phosphoethanolamine transferase enzyme family expressed in E. coli and responsible for modification of lipid A with phosphoethanolamine [33].…”

Section: Hfbri-25 Effectively Kills Uropathogenic E Coli Strains and ...mentioning

confidence: 99%

Novel BRICHOS-Related Antimicrobial Peptides from the Marine Worm Heteromastus filiformis: Transcriptome Mining, Synthesis, Biological Activities, and Therapeutic Potential

Panteleev,

Safronova,

Duan

et al. 2023

Marine Drugs

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Marine polychaetes represent an extremely rich and underexplored source of novel families of antimicrobial peptides (AMPs). The rapid development of next generation sequencing technologies and modern bioinformatics approaches allows us to apply them for characterization of AMP-derived genes and the identification of encoded immune-related peptides with the aid of genome and transcriptome mining. Here, we describe a universal bioinformatic approach based on the conserved BRICHOS domain as a search query for the identification of novel structurally unique AMP families in annelids. In this paper, we report the discovery of 13 novel BRICHOS-related peptides, ranging from 18 to 91 amino acid residues in length, in the cosmopolitan marine worm Heteromastus filiformis with the assistance of transcriptome mining. Two characteristic peptides with a low homology in relation to known AMPs—the α-helical amphiphilic linear peptide, consisting of 28 amino acid residues and designated as HfBRI-28, and the 25-mer β-hairpin peptide, specified as HfBRI-25 and having a unique structure stabilized by two disulfide bonds—were obtained and analyzed as potential antimicrobials. Interestingly, both peptides showed the ability to kill bacteria via membrane damage, but mechanisms of their action and spectra of their activity differed significantly. Being non-cytotoxic towards mammalian cells and stable to proteolysis in the blood serum, HfBRI-25 was selected for further in vivo studies in a lethal murine model of the Escherichia coli infection, where the peptide contributed to the 100% survival rate in animals. A high activity against uropathogenic strains of E. coli (UPEC) as well as a strong ability to kill bacteria within biofilms allow us to consider the novel peptide HfBRI-25 as a promising candidate for the clinical therapy of urinary tract infections (UTI) associated with UPEC.

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Section: Hfbri-25 Effectively Kills Uropathogenic E Coli Strains and ...mentioning

confidence: 99%

Novel BRICHOS-Related Antimicrobial Peptides from the Marine Worm Heteromastus filiformis: Transcriptome Mining, Synthesis, Biological Activities, and Therapeutic Potential

Panteleev,

Safronova,

Duan

et al. 2023

Marine Drugs

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Dissemination of MCR-Type Genes Encoding Polymyxin Resistance in Russia and Across the Globe

Ageevets¹,

Sulyan

Lazareva³

et al. 2021

A&Ch

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The MCR-type polymyxin resistance gene localized on a mobile genetic element was described was described in 2015 for the first time. Publications, following the first description, demonstrated the global distribution of MCR-type genes and the role of animal husbandry in this process. At the same time, practical importance of polymyxins is growing, considering spread of genes of acquired resistance to carbapenem antibiotics. This review briefly summarizes the principal data on this issue.

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Phenotypic and molecular genetic properties of Escherichia coli clinical strains isolated from patients with urological diseases

Cited by 2 publications

References 0 publications

Novel BRICHOS-Related Antimicrobial Peptides from the Marine Worm Heteromastus filiformis: Transcriptome Mining, Synthesis, Biological Activities, and Therapeutic Potential

Novel BRICHOS-Related Antimicrobial Peptides from the Marine Worm Heteromastus filiformis: Transcriptome Mining, Synthesis, Biological Activities, and Therapeutic Potential

Dissemination of MCR-Type Genes Encoding Polymyxin Resistance in Russia and Across the Globe

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