Phenotypic and molecular insights into heat tolerance of formulated cells as active ingredients of fungal insecticides

Tong, Sen‐Miao; Feng, Ming‐Guang

doi:10.1007/s00253-020-10659-z

Cited by 41 publications

(30 citation statements)

References 120 publications

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“…The H3K_me-dependent gene expression and phenotypes are of special merits for the pest control potential of fungal insect pathogens since the potential relies upon fungal virulence, stress tolerance and developmental traits favoring massive production of qualified conidia as active ingredients of fungal insecticides [46][47][48]. In Metarhizium robertsii, abolishment of H3K4me by loss-of-function mutation of SET1/KMT2 resulted in blocked appressorial formation, compromised insect pathogenicity, and differential expression of 1,498 genes considered to be KMT2 dependent [49].…”

Section: Introductionmentioning

confidence: 99%

DIM5/KMT1 controls fungal insect pathogenicity and genome stability by methylation of histone H3K4, H3K9 and H3K36

et al. 2021

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Mono-, di-and tri-methylation of histone H3 Lys 9, Lys 4, and Lys 36 (H3K_me1/me2/me3) required for mediation of DNA-based cellular events in eukaryotes usually rely upon the activities of histone lysine methyltransferases (KMTs) classified to the KMT1, KMT2, and KMT3 families, respectively. Here, an H3K9-specific DIM5/KMT1 orthologue, which lacks a C-terminal post-SET domain and localizes mainly in nucleus, is reported to have both conserved and noncanonical roles in methylating the H3 core lysines in Beauveria bassiana, an insect-pathogenic fungus serving as a main source of wide-spectrum fungal insecticides. Disruption of dim5 led to abolishment of H3K9me3 and marked attenuation of H3K4me1/me2, H3K9me1/me2 and H3K36me2. Consequently, the Δdim5 mutant lost the whole insect pathogenicity through normal cuticle infection, and was compromised severely in virulence through cuticle-bypassing infection (hemocoel injection) and also in a series of cellular events critical for the fungal virulence and lifecycle in vivo and in vitro, including reduced hyphal growth, blocked conidiation, impeded proliferation in vivo, altered carbohydrate epitopes, disturbed cell cycle, reduced biosynthesis and secretion of cuticle-degrading enzymes, and increased sensitivities to various stresses. Among 1,201 dysregulated genes (up/down ratio: 712:489) associated with those phenotypic changes, 92 (up/down ratio: 59:33) encode transcription factors and proteins or enzymes involved in posttranslational modifications, implying that the DIM5-methylated H3 core lysines could act as preferential marks of those transcription-active genes crucial for global gene regulation. These findings uncover a novel scenario of DIM5 and its indispensability for insect-pathogenic lifestyle and genome stability of B. bassiana.

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Section: Introductionmentioning

confidence: 99%

DIM5/KMT1 controls fungal insect pathogenicity and genome stability by methylation of histone H3K4, H3K9 and H3K36

et al. 2021

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“…Since Hsp78 and Hsp104 have been reported to be two crucial HSPs in thermal resistance capability in S. cerevisiae (Leidhold et al, 2006;Bandyopadhyay et al, 2019), we speculate that the enhanced thermal resistance ability may be caused by the increased HSP expression and the hypothesis was partly confirmed by the higher upregulated transcription levels of two key HSP genes in the mutant strains in comparison with those in control strains. Besides, a previous review has shown that hyphal trehalose content increases sharply after 6-h exposures to 35, 37.5 and 40 C in B. bassiana (Tong and Feng, 2020). Also, studies have revealed that chitin can be synthesized from trehalose in fungi (Gow et al, 2017).…”

Section: Discussionmentioning

confidence: 97%

“…Besides, a previous review has shown that hyphal trehalose content increases sharply after 6‐h exposures to 35, 37.5 and 40°C in B . bassiana (Tong and Feng, 2020). Also, studies have revealed that chitin can be synthesized from trehalose in fungi (Gow et al ., 2017).…”

Section: Discussionmentioning

confidence: 99%

Dsk2 involves in conidiation, multi‐stress tolerance and thermal adaptation in Beauveria bassiana

Wang

Guan

et al. 2021

Environ Microbiol Rep

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Dsk2 is a nuclear-enriched ubiquitin-like polyubiquitinbinding protein that regulates protein degradation in yeast but has not been explored yet in filamentous fungi, such as Beauveria bassiana. Here, we report Beauveria bassiana Dsk2 located both in the nucleus and in cytoplasm of hyphal cells. Deletion of Dsk2 resulted in mild growth defect on scant media with various carbon/nitrogen sources and dramatic attenuation in conidiation capability at optimal condition. Compared to the wild-type, ΔDsk2 strains are much more sensitive to high osmotic and oxidative pressure during vegetative growth. Meanwhile, the mutant strains showed an increased chemical tolerance to Congo red and calcofluor white, two cell wall perturbing agents. The transcriptional changes of genes involved in central development, superoxide dismutase and chitin synthesis pathway indicate that Dsk2 acts as a multifunctional regulator in adapting to environmental changes. Importantly, Dsk2 negatively regulated the ability of thermal resistance in B. bassiana, which makes it a potential target gene for constructing engineering anti-thermal strains in the circumstance of global warming. Altogether, our finding highlights novel roles of Dsk2 involved in the asexual cycle, multi-stress tolerance and pest control potential of B. bassiana.

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“…Heat stress could promote cellular ROS generation, thereby damaging certain biomolecules, such as DNA, lipids or proteins [33,36]. Antioxidant enzymes can scavenge diverse intracellular ROS [27,37]. Typically, SOD can decompose superoxide anions and produce H 2 O 2 and oxygen [38], while H 2 O 2 can be additionally decomposed to oxygen and water via catalase [39].…”

Section: Plos Onementioning

confidence: 99%

A p53-like transcription factor, BbTFO1, contributes to virulence and oxidative and thermal stress tolerances in the insect pathogenic fungus, Beauveria bassiana

et al. 2021

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The p53-like transcription factor (TF) NDT80 plays a vital role in the regulation of pathogenic mechanisms and meiosis in certain fungi. However, the effects of NDT80 on entomopathogenic fungi are still unknown. In this paper, the NDT80 orthologue BbTFO1 was examined in Beauveria bassiana, a filamentous entomopathogenic fungus, to explore the role of an NDT80-like protein for fungal pest control potential. Disruption of BbTFO1 resulted in impaired resistance to oxidative stress (OS) in a growth assay under OS and a 50% minimum inhibitory concentration experiment. Intriguingly, the oxidation resistance changes were accompanied by transcriptional repression of the two key antioxidant enzyme genes cat2 and cat5. ΔBbTFO1 also displayed defective conidial germination, virulence and heat resistance. The specific supplementation of BbTFO1 reversed these phenotypic changes. As revealed by this work, BbTFO1 can affect the transcription of catalase genes and play vital roles in the maintenance of phenotypes associated with the biological control ability of B. bassiana.

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Phenotypic and molecular insights into heat tolerance of formulated cells as active ingredients of fungal insecticides

Cited by 41 publications

References 120 publications

DIM5/KMT1 controls fungal insect pathogenicity and genome stability by methylation of histone H3K4, H3K9 and H3K36

DIM5/KMT1 controls fungal insect pathogenicity and genome stability by methylation of histone H3K4, H3K9 and H3K36

Dsk2 involves in conidiation, multi‐stress tolerance and thermal adaptation in Beauveria bassiana

A p53-like transcription factor, BbTFO1, contributes to virulence and oxidative and thermal stress tolerances in the insect pathogenic fungus, Beauveria bassiana

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