Phenotypic and Molecular Survey of Metallo-beta-lactamase-producing Pseudomonas aeruginosa Isolated from Patients with Nosocomial and Non- Nosocomial Infections

Abstract

Background: Resistance to antimicrobial agents in Pseudomonas aeruginosa (P. aeruginosa) including carbapenems is a prominent problem in patients. The aim of this study is surveying Metallo-beta-lactamase (MBL)-producing P. aeruginosa isolated from patient specimens with nosocomial and non-nosocomial infections in Kurdistan province, Iran. Methods: In total, 146 Pseudomonas spp. were collected (December 2015 to August 2017). P. aeruginosa isolates were detected by phenotypic and polymerase chain reactions (PCR) of gyrB gene. Combination disk (CD) phenotypic test was used for the identification of MBL-producing strains and PCR was applied for identification of blaIMP and blaVIM genes in P. aeruginosa. Sensitivity and specificity of phenotypic tests were calculated as well. Fisher’s exact test and logistic regression were used for data analysis (p≤0.05). Results: A total of 134 (91.78%) and 133 (91.09%) P. aeruginosa were detected using PCR and the phenotypic test, respectively. Fifty-six (41.79%) clinical isolates were isolated from patients with nosocomial infection. CD test proved that 67 out of 134 (50%) P. aeruginosa isolates were positive for MBL, of which 11 (8.20%) carried blaIMP gene. No significant relationship was found between MBL-producing P. aeruginosa and blaIMP genes; as well as between MBL-producing P. aeruginosa and blaIMP genes with age, sex, city of residence, inpatient/outpatient and specimen's type (p≥0.05). Conclusion: Presence of MBL-producing P. aeruginosa strains and blaIMP genes were proved in this study; thus more precaution should be taken in the administration of carbapenem antibiotics to patients.

relBE toxin-antitoxin system as a reliable anti-biofilm target in Pseudomonas aeruginosa

“…It should be noted that clinical isolates of P. aeruginosa were isolated from urine and tracheal infections. Also, these isolates were confirmed as P. aeruginosa through phenotypic and molecular methods (Rouhi & Ramazanzadeh., 2020).…”

Contribution of Beta-Lactamases and Efflux Pumps to Multidrug Resistance inPseudomonas aeruginosaIsolates from ICU Patients in Northeast Brazil

“…However, a study carried out by Han and colleagues [42], analyzing strains of A. baumannii resistant to carbapenems in a university hospital in China, found an association between the presence of the bla TEM and bla OXA-23-like genes with the carbapenem resistance phenotype. In research carried out in an Iranian hospital, from 2015 to 2017, resistant strains of P.aeruginosa were isolated from patients with nosocomal and non-nosocomial infections and the presence of the bla OXA-23-like gene was identified in 11.19% of the isolates [43], evidencing the circulation of the OXA group gene in species other than Acinetobacter .…”

High Prevalence of Antiseptic Resistance Encoding Genes and Reduced Phenotypic Antiseptic Susceptibility Among Antibiotic-Resistant Pseudomonas aeruginosa Isolates

“…All isolates were confirmed at the gene level as P. aeruginosa by detecting the gyrB gene using polymerase chain reaction (PCR) (20). All isolates were screened for antiseptic and antibiotic resistance genes such as qacE, qacE∆1, and blaOXA-23 genes as described before (21,22). The Supplementary File shows the primer sequences and related PCR protocols.…”