Phenotypic and transcriptional responses associated with multi-generation exposure of <i>Folsomia candida</i> to engineered nanomaterials

Noordhoek, Jeroen W.; Pipicelli, Fabrizia; Barone, Ida; Franken, Oscar; Montagne-Wajer, Kora; Mariën, Janine; Verweij, Rudo A.; Gestel, Cornelis A.M. van; Straalen, Nico M. van; Roelofs, Dick

doi:10.1039/c8en00456k

Cited by 12 publications

(7 citation statements)

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“…As the field case always involves “multi-generational” exposure, some potential sub-lethal effects of an environmental factor may cumulate over time and become apparent only in a living organism after exposure over multiple generations. Noordhoek et al (2018) found a metal-based nanomaterial tungsten carbide-cobalt affected the reproduction and survival of F. candida from the third generation. By using multi-generational laboratory risk assessments, Bakonyi et al (2011) and Szabó, Seres & Bakonyi (2017) found that F. candida that were fed Cry1Ab expressing maize leaves had some alterations in life-history traits and reproduction.…”

Section: Introductionmentioning

confidence: 99%

“…Based on RNA-Seq data, Zhang et al (2017b) annotated 32,268 unigenes of the Chinese green mussel ( Perna viridis ) and identified 9,048 differentially expressed genes (DEGs) between exposed and non-exposed groups to cadmium, suggesting a sensitive response of the mussel transcriptome to cadmium. Noordhoek et al (2018) studied phenotypic effects and transcriptional responses in F. candida exposed to nanomaterials and detected physiological alterations cumulating from one generation to the next based on gene expression assays but without visible phenotypic variables.…”

Section: Introductionmentioning

confidence: 99%

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A multi-generational risk assessment of Cry1F on the non-target soil organismFolsomia candida(Collembola) based on whole transcriptome profiling

Huang

Chen

et al. 2019

PeerJ

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The Bacillus thuringiensis toxin Cry1F has been used to develop insect-resistant genetically engineered crops. There has been great interest in evaluating its potential risk to non-target organisms (NTOs). However, the majority of previous risk assessments only examined one generation of NTOs using several physiological indicators, which cannot comprehensively detect some potential sub-lethal effects at the molecular level. In this study, we conducted a laboratory-based, multi-generational risk assessment of Cry1F for the collembolan Folsomia candida, an important representative of soil arthropods in terms of survival, reproduction, and differentially expressed genes (DEGs) identified from whole transcriptome profiles. Our results demonstrated that Cry1F was continuously ingested by collembolans over three consecutive generations, but it did not affect the survival or reproduction of F. candida. There were no significant differences in the global gene expression between F. candida—fed diets with and without Cry1F, and no consistent co-expressed DEGs over three generations. In addition, Cry1F did not obviously alter the expression profiles of seven sensitive biological markers. Our composite data indicates that Cry1F had no long-term harmful effects on collembolan F. candida.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A multi-generational risk assessment of Cry1F on the non-target soil organismFolsomia candida(Collembola) based on whole transcriptome profiling

Huang

Chen

et al. 2019

PeerJ

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show abstract

“…As the field case always involves "multi-generational" exposure, some potential sub-lethal effects of an environmental factor may cumulate over time and become apparent only in a living organism after exposure over multiple generations. Noordhoek et al (2018) found a metal-based nanomaterial tungsten carbide-cobalt affected the reproduction and survival of F. candida from the third generation. The current one-generation laboratory risk assessment cannot comprehensively evaluate the potential nonlethal chronic effects of Bt toxins on NTOs.…”

Section: Introductionmentioning

confidence: 99%

Peer Review #3 of "A multi-generational risk assessment of Cry1F on the non-target soil organism Folsomia candida (Collembola) based on whole transcriptome profiling (v0.1)"

Storer¹

2019

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The Bacillus thuringiensis (Bt) toxin Cry1F has been used to develop insect-resistant genetically engineered (IRGE) crops. There has been great interest in evaluating its potential risk to non-target organisms (NTOs). However, the majority of previous risk assessments only examined one generation of NTOs using several physiological indicators, which cannot comprehensively detect some potential sub-lethal effects at the molecular level. In this study, we conducted a laboratory-based, multi-generational risk assessment of Cry1F for the collembolan Folsomia candida, an important representative of soil arthropods in terms of survival, reproduction, and differentially expressed genes (DEGs) identified from whole transcriptome profiles. Our results demonstrated that Cry1F was continuously ingested by collembolans over three consecutive generations, but it did not affect the survival or reproduction of F. candida. There were no significant differences in the global gene expression between F. candida-fed diets with and without Cry1F, and no consistent co-expressed DEGs over three generations. In addition, Cry1F did not obviously alter the expression profiles of seven sensitive biological markers. Our composite data indicates that Cry1F had no long-term harmful effects on collembolan F. candida.

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“…Concerning terrestrial organisms, metal NPs, such as copper oxide and silver, showed mechanisms of toxicity in longer-term exposures that were not predictable based on short-term studies [ 28 , 29 ]. Some longer-term studies assessing the toxicity of different NPs to soil organisms are available (e.g., E. crypticus [ 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 ] ( Table 1 ) and Folsomia candida [ 38 ]).…”

Section: Introductionmentioning

confidence: 99%

Environmental Hazards of Boron and Vanadium Nanoparticles in the Terrestrial Ecosystem—A Case Study with Enchytraeus crypticus

et al. 2021

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From the start of the 21st century, nanoecotoxicological research has been growing in fast steps due to the need to evaluate the safety of the increasing use of engineered nanomaterials. Boron (B) and vanadium (V) nanoparticles (NPs) generated by anthropogenic activities are subsequently released in the environment; therefore, organisms can be continuously exposed to these NPs for short or long periods. However, the short and long-term effects of BNPs and VNPs on soil organisms are unknown. This work aimed to recognize and describe their potential toxicological effects on the model species Enchytraeus crypticus, assessing survival and reproduction, through a longer-term exposure (56 days (d)—OECD test extension of 28 d), and avoidance behavior, through a short-term exposure (48 hours (h)). After 28 d, BNPs did not induce a significant effect on E. crypticus survival, whereas they decreased the organisms’ reproduction at 500 mg/kg. From 10 to 500 mg/kg, VNPs decreased the E. crypticus survival and/or reproduction. After 56 d, 100 to 500 mg/kg BNPs and 50 to 500 mg/kg VNPs, decreased the reproduction output of E. crypticus. The estimated Effect Concentrations (ECx) based on reproduction, for BNPs, were lower at 56 d compared with 28 d; for VNPs, an opposite pattern was found: ECx 28 d < ECx 56 d. BNPs did not induce an avoidance behavior, but organisms avoided the soil contaminated with 10 mg VNPs/kg. The tested NPs showed different E. crypticus apical effects at 28 d from the ones detected at 56 d, dependent on the type of NPs (B vs. V). In general, VNPs showed to be more toxic than BNPs. However, the effects of VNPs were alleviated during the time of exposure, contrarily to BNPs (which became more toxic with extended duration). The present study adds important information about NPs toxicity with ecological significance (at the population level). Including long-term effects, the obtained results contributes to the improvement of NPs risk assessment.

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Phenotypic and transcriptional responses associated with multi-generation exposure of Folsomia candida to engineered nanomaterials

Abstract: This article shows the phenotypic and transcriptional effects of multi-generation nanomaterial exposure to the springtail Folsomia candida.

Cited by 12 publications

References 46 publications

A multi-generational risk assessment of Cry1F on the non-target soil organismFolsomia candida(Collembola) based on whole transcriptome profiling

A multi-generational risk assessment of Cry1F on the non-target soil organismFolsomia candida(Collembola) based on whole transcriptome profiling

Peer Review #3 of "A multi-generational risk assessment of Cry1F on the non-target soil organism Folsomia candida (Collembola) based on whole transcriptome profiling (v0.1)"

Environmental Hazards of Boron and Vanadium Nanoparticles in the Terrestrial Ecosystem—A Case Study with Enchytraeus crypticus

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