Phenotypic Broad Spectrum of Bacterial Blight Disease Resistance from Thai Indigenous Upland Rice Germplasms Implies Novel Genetic Resource for Breeding Program

Chumpol, Atitaya; Monkham, Tidarat; Saepaisan, Suwita; Sanitchon, Jirawat; Falab, Shanerin; Chankaew, Sompong

doi:10.3390/agronomy12081930

Cited by 4 publications

(1 citation statement)

References 54 publications

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“…The scoring was based on the standard method of the International Rice Research Institute (IRRI) [17,[50][51][52][53]. Four groups of scoring were established based on LL, with lesion lengths ranging from 0-5 cm considered resistant, >5-10 cm moderately resistant, >10-15 cm moderately susceptible, and more than 15 cm susceptible [17,50,54]. The average value of lesion length was used as the phenotypic data for the GWAS analysis.…”

Section: Evaluation Of Bacterial Leaf Blight Resistance In Rice Plantmentioning

confidence: 99%

Genome-Wide Association Study Using Genotyping by Sequencing for Bacterial Leaf Blight Resistance Loci in Local Thai Indica Rice

Danaisilichaichon

Vejchasarn²,

Patarapuwadol

et al. 2023

Agronomy

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Bacterial leaf blight (BLB) is a devastating disease caused by Xanthomonas oryzae pv. oryzae (Xoo), which poses a significant threat to global rice production. In this study, a genome-wide association study (GWAS) was conducted using the genotyping-by-sequencing (GBS) approach to identify candidate single nucleotide polymorphisms (SNPs) associated with BLB resistance genes. The study utilized 200 indica rice accessions inoculated with seven distinct Xoo isolates and filtered highly significant SNPs using a minor allele frequency (MAF) of >5% and a call rate of 75%. Four statistical models were used to explore potential SNPs associated with BLB resistance, resulting in the identification of 32 significant SNPs on chromosomes 1–8 and 12 in the rice genome. Additionally, 179 genes were located within ±100 kb of the SNP region, of which 49 were selected as candidate genes based on their known functions in plant defense mechanisms. Several candidate genes were identified, including two genes in the same linkage disequilibrium (LD) decay as the well-known BLB resistance gene (Xa1). These findings represent a valuable resource for conducting further functional studies and developing novel breeding strategies to enhance the crop’s resistance to this disease.

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Section: Evaluation Of Bacterial Leaf Blight Resistance In Rice Plantmentioning

confidence: 99%

Genome-Wide Association Study Using Genotyping by Sequencing for Bacterial Leaf Blight Resistance Loci in Local Thai Indica Rice

Danaisilichaichon

Vejchasarn²,

Patarapuwadol

et al. 2023

Agronomy

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MEMBRANE PROTEIN 1 encoding an amino acid transporter confers resistance to blast fungus and leaf-blight bacterium in rice

Jiang,

Huang,

Wang

et al. 2024

Journal of Experimental Botany

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Amino acid homeostasis is interconnected with the immune network of plants. During plant-pathogen interaction, amino acid transporters (AATs) have been shown to be involved in plant immune responses. However, the molecular mechanism by which how AATs function in this process remains elusive. In this study, we identify OsMP1 that acts as a quantitative trait locus against blast fungus from a joint analysis of GWAS and QTL mapping in rice. Heterogeneous expression of OsMP1 in yeast supports its function in transporting a wide range of amino acids, including Thr, Ser, Phe, His and Glu. OsMP1 could also mediate 15N-Glu efflux and influx in Xenopus oocyte cells. The expression of OsMP1 is dramatically induced by Magnaporthe oryzae in the resistant landrace Heikezijing, while remaining unresponsive in the susceptible landrace Suyunuo. Overexpressing OsMP1 in Suyunuo enhances disease resistance to blast fungus and leaf-blight bacterium without yield penalty. Furthermore, the overexpression of OsMP1 leads to increased accumulation of Thr, Ser, Phe and His in the leaves. And the heightened levels of these amino acids contribute to reduced disease susceptibility, which is associated with upregulated jasmonic acid pathway. Thus, our results elucidate the pivotal role of OsMP1 in disease resistance and provide a potential target for breeding more resistant rice cultivars without compromising yield.

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Broad-Spectrum Resistance and Monogenic Inheritance of Bacterial Blight Resistance in an Indigenous Upland Rice Germplasm ULR207

Wongsa,

Chankaew,

Monkham

et al. 2024

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Bacterial blight (BB) caused by Xanthomonas oryzae pv. Oryzae (Xoo) is a serious disease of rice worldwide that can reduce crop yield and affect food insecurity. A rice resistance variety is an alternate way to solve this problem. The broad-spectrum resistance (BSR) of ULR207 is important for durable resistance to several of the Xoo isolates. However, the inheritance of this resistance gene in ULR207 must be known before it can be utilized. Thus, this study aimed to survey the BB resistance gene with reference to the BB resistance gene for identification of non-analogous or analogous genes and confirmation of a broad-spectrum resistance, to investigate the gene effect, the number of genes, and the heritability of the BB resistance gene in the ULR207 variety. Six populations of two crosses (Maled Phai × ULR207 and RD6 × ULR207), i.e., ULR207 (Donor parent), Maled Phai and RD6 (Recurrent parent), F1, F2, BC1P1, and BC1P2 were constructed. These materials were evaluated for BB resistance by clipping methods under greenhouse conditions using a virulence isolate of a pathogen in Thailand. The results showed that ULR207 exhibited the strongest against BB with 0.8 of BSR with low area under the disease progress curve (AUDPC). Molecular screening for surveying of the BB resistance gene in ULR207 revealed a non-analogous resistance gene with resistance check varieties. The phenotype of the disease lesion length of F2 and BC1P2 populations exhibited a ratio of 1:3 and 1:1 (resistant: susceptible), respectively, revealing a single recessive gene in both crosses. The scaling test parameters A, B, and C were non-significant (p < 0.01), indicating that variation in data was sufficiently explained by additive (d) and dominance (h) components. The gene action of ULR207 was controlled by additive gene action. Heritability of the two crosses, Maled Phai x ULR207 and RD6 x ULR207, exhibited high values with 0.817 and 0.716, whereas the numbers of the genes were 1.4 and 1.2, respectively. The result indicated that the breeding strategy could be employed in early generations when using ULR207 as a new source of bacterial blight resistance.

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Phenotypic Broad Spectrum of Bacterial Blight Disease Resistance from Thai Indigenous Upland Rice Germplasms Implies Novel Genetic Resource for Breeding Program

Cited by 4 publications

References 54 publications

Genome-Wide Association Study Using Genotyping by Sequencing for Bacterial Leaf Blight Resistance Loci in Local Thai Indica Rice

Genome-Wide Association Study Using Genotyping by Sequencing for Bacterial Leaf Blight Resistance Loci in Local Thai Indica Rice

MEMBRANE PROTEIN 1 encoding an amino acid transporter confers resistance to blast fungus and leaf-blight bacterium in rice

Broad-Spectrum Resistance and Monogenic Inheritance of Bacterial Blight Resistance in an Indigenous Upland Rice Germplasm ULR207

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