Phenotypic characterization of ESBL producing Enterobacter cloacae among children

Amin, Hafsa; Zafar, Ali; Ejaz, Hasan; Jameel, Noor Ul Ain

doi:10.12669/pjms.291.2385

Cited by 14 publications

(12 citation statements)

References 19 publications

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“…was 12.73% which was different from the other results in Pakistan (50%, 14.93%) (21,22), Nigeria (37.5%) (10) and Pennsylvania, the USA (33.33%) (9).…”

Section: Discussioncontrasting

confidence: 67%

Emergence of blaCTX-M-15 Gene and Its Transferability in Enterobacter spp. Isolated From the Hospitals of Tehran, Iran

Rizi¹,

Peerayeh²,

Bakhshi³

et al. 2015

Avicenna J Clin Microbiol Infect

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Background: Enterobacter spp. is increasingly recognized as an important nosocomial pathogen and implicated in many episodes of hospital acquired infections. Objectives: The current study aimed to describe distribution and transferability of bla CTX-M-15 gene, and the antibiotic susceptibility pattern in the clinical isolates of Enterobacter spp. Materials and Methods: A total of 110 Enterobacter isolates were collected from five hospitals in Tehran, Iran from May 2012 to April 2013. Enterobacter species were identified by API 20E system. Antibacterial susceptibility was determined by disk diffusion method, and extended spectrum beta lactamase (ESBL) production was confirmed by combined disk method. The bla CTX-M-15 gene was identified by PCR with specific primers. The transferability of the bla CTX-M-15 was tested by conjugation with broth matting method. Results: The prevalence of Enterobacter species was E. cloacae (78.2 %), E. aerogenes (6.13 %) and E. sakazakii (8.2%). They were from different clinical sources. Maximal resistance in Enterobacter isolates was noticed against Augmentin®, trimethoprim -sulfamethoxazole and cefoxitin 75.5%, 64.5%, and 59.1%, respectively. Fourteen isolates, showed ESBL phenotype. The bla CTX-M-15 gene frequency in Enterobacter isolates was 11.8%. Three conjugative plasmids containing bla CTX-M-15 were found in one Enterobacter isolate.

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“…was 12.73% which was different from the other results in Pakistan (50%, 14.93%) (21,22), Nigeria (37.5%) (10) and Pennsylvania, the USA (33.33%) (9).…”

Section: Discussioncontrasting

confidence: 67%

Emergence of blaCTX-M-15 Gene and Its Transferability in Enterobacter spp. Isolated From the Hospitals of Tehran, Iran

Rizi¹,

Peerayeh²,

Bakhshi³

et al. 2015

Avicenna J Clin Microbiol Infect

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“…E. coli strains have been found resistant to co-trimoxazole (63%), ampicillin, and tetracycline (87% each) in a different study 26 . Massive self-medication and indiscriminately advised antibacterial drugs without scientific investigations could result in multi-drug resistant Gram-negative pathogens, particularly against cephalosporins, aminoglycosides, and fluoroquinolones in the wound as well as in other infections 20,31,32 . The production of AmpC, extended-spectrum betalactamases, and imipenemases among the Gramnegative isolates explicit the resistance against expanded spectrum cephalosporins [33][34][35] .…”

Section: Discussionmentioning

confidence: 99%

Determination of Bacterial Profile and Spectrum of Antimicrobial Drug Resistance in Pediatric Wound Infections

Ejaz¹

2019

J Pure Appl Microbiol

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Pediatric wound infections lead to the prolonged morbidity of the child and financial burden for the parents. The present study aimed to determine the causative pathogens and the spectrum of their drug resistance to reduce the fatality in pediatric wound infections. The study conducted prospectively on wound specimens collected from a pediatric facility of Lahore, Pakistan. The specimens were processed on routine culture media, and the conventional biochemicals, analytical profile index (API) 20 E, and 20 NE identified the organisms. The spectrum of drug resistance observed against several classes of antibiotics. Of 960 cases, 695 (72.4%) were culture positive and 265 (27.6%) culture-negative cases. The pediatric cases of < 1 year of age had significant (p = 0.01) association with negative cultures. Illiteracy and primary education of the parents were significantly (p < 0.01 and 0.01, respectively) associated with pediatric wound infections. The distribution of the bacterial pathogens demonstrated 216 (27.6%) Gram-positive and 568 (72.4%) Gram-negative bacteria. Gram-positive isolates were resistant to most of the penicillins, co-trimoxazole, and cephalosporins, while none of these was resistant to linezolid and vancomycin. The majority of Gram-negative bacteria were resistant to cephalosporins and aminoglycosides, while lesser resistance observed against carbapenems, piperacillin-tazobactam, and cefoperazone-sulbactam. The wound infections could be polymicrobial with the expanded spectrum of drug resistance to most of the penicillin, cephalosporins, aminoglycosides, and fluoroquinolones. These infections lead to serious pediatric morbidity if not treated meticulously.

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“…The test procedure is as follows: ceftazidime/clavulanate and cefotaxime/clavulanate discs, in comparison with ceftazidime and cefotaxime discs alone (Oxoid Ltd., Basingstoke, Unite Kingdom). According to the CLSI 2019 antimicrobial susceptibility testing standards [ 19 ], EPE was defined when an increase in a zone diameter ≥ 5 mm for either antibiotic together with clavulanate vs the zone diameter of the agent, and it is reported that the ESBL phenotype of other Enterobacteriaceae can also be determined by the same method as the above four Enterobacteriaceae [ 20 ]. The left were defined as non-ESBL-producing Enterobacteriaceae (NEPE).…”

Section: Methodsmentioning

confidence: 99%

Phenotype, molecular characterisation and risk factors for postoperative meningitis caused by ESBL-producing-Enterobacteriaceae: a six years multi-Centre comparative cohort study

et al. 2021

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Background To determine the phenotype, molecular characterisation and risk factors of postoperative meningitis induced by Extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae (EPE) in China. Methods We performed a multi-centre comparative cohort study of postoperative meningitis patients infected with Enterobacteriaceae in 4 neurosurgical centres in China from January 2014 to December 2019. Phenotype and molecular characteristics of the isolates were reviewed and tested, and independent risk factors of the EPE meningitis were evaluated by binary logistic regression. Results In total, 220 Enterobacteriaceae include 78 EPE were available in this study. 85.6% (67/78) ESBL-related genes were tested, and blaSHV (14.9%) and blaSHV + blaTEM + blaCTX-M-9 (20.9%) were found to be the most frequent mono and combined ESBL-related genes harboured by Enterobacteriaceae. On binary logistic analysis, craniotomy (OR. 2.583, 95% C.I. 1.274–5.235, P = 0.008) and malignancy (OR. 2.406, 95% C.I. 1.299–4.456, P = 0.005) were the associated independent risk factors to meningitis induced by EPE. Conclusions To the best of our knowledge, this is the largest series focusing on risk factors of EPE meningitis which has been conducted in China. Craniotomy and malignancy were independent risk factors for EPE meningitis. The risk factors identified may be further utilized in clinical practice and research to avoid and reduce the mortality in future.

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Phenotypic characterization of ESBL producing Enterobacter cloacae among children

Cited by 14 publications

References 19 publications

Emergence of blaCTX-M-15 Gene and Its Transferability in Enterobacter spp. Isolated From the Hospitals of Tehran, Iran

Emergence of blaCTX-M-15 Gene and Its Transferability in Enterobacter spp. Isolated From the Hospitals of Tehran, Iran

Determination of Bacterial Profile and Spectrum of Antimicrobial Drug Resistance in Pediatric Wound Infections

Phenotype, molecular characterisation and risk factors for postoperative meningitis caused by ESBL-producing-Enterobacteriaceae: a six years multi-Centre comparative cohort study

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