Phenotypic detection and antibiogram of β-lactamase-producing proteus species in a tertiary care hospital, India

N, Pal; Hooja, Saroj; Sharma, Rameshwar K.; Maheshwari, RK

doi:10.4103/amhsr.amhsr_413_15

Cited by 21 publications

(12 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bacterial resistance is one of the major public health problems. It includes the emergence of MDR pathogens, such as resistant isolates of Proteus mirabilis , which have been associated with urinary tract infections and nosocomial infections worldwide [ 4 , 38 ]. P. mirabilis isolates have been reported to be resistant to penicillins [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

“…P. mirabilis isolates have been reported to be resistant to penicillins [ 39 ]. They also show a high incidence of resistance to cephalosporins, carbapenems, and quinolones [ 4 , 40 ]. In the current study, a high percentage of the isolates was resistant or intermediately resistant to amoxicillin-clavulanic acid and cefotaxime.…”

Section: Discussionmentioning

confidence: 99%

“…It is part of the normal gut flora and can cause opportunistic infections in immunocompromised and elderly patients [ 3 ]. The emergence of antibiotic resistance in clinical isolates of P. mirabilis is a major healthcare issue [ 4 , 5 ]. P. mirabilis poses a major challenge in infection management as it produces AmpC β-lactamases and extended spectrum β-lactamases [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Antimicrobial and Antibiofilm Activities of Probiotic Lactobacilli on Antibiotic-Resistant Proteus mirabilis

et al. 2020

View full text Add to dashboard Cite

The emergence of biofilm-forming, multi-drug-resistant (MDR) Proteus mirabilis infections is a serious threat that necessitates non-antibiotic therapies. Antibiotic susceptibility and biofilm-forming activity of P. mirabilis isolates from urine samples were assessed by disc diffusion and crystal violet assays, respectively. Antimicrobial activities of probiotic Lactobacilli were evaluated by agar diffusion. Antibiofilm and anti-adherence activities were evaluated by crystal violet assays. While most P. mirabilis isolates were antibiotic-resistant to varying degrees, isolate P14 was MDR (resistant to ceftazidime, cefotaxime, amoxicillin-clavulanic acid, imipenem, ciprofloxacin, and amikacin) and formed strong biofilms. Cultures and cell-free supernatants of Lactobacillus casei and Lactobacillus reuteri exhibited antimicrobial and antibiofilm activities. The 1/16 concentration of untreated supernatants of L. casei and L. reuteri significantly reduced mature biofilm formation and adherence of P14 by 60% and 72%, respectively (for L. casei), and by 73% each (for L. reuteri). The 1/8 concentration of pH-adjusted supernatants of L. casei and L. reuteri significantly reduced mature biofilm formation and adherence of P14 by 39% and 75%, respectively (for L. casei), and by 73% each (for L. reuteri). Scanning electron microscopy (SEM) confirmed eradication of P14’s biofilm by L. casei. L. casei and L. reuteri could be utilized to combat Proteus-associated urinary tract infections.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Antimicrobial and Antibiofilm Activities of Probiotic Lactobacilli on Antibiotic-Resistant Proteus mirabilis

et al. 2020

View full text Add to dashboard Cite

show abstract

“… 29 A high prevalence of ESBL and AmpC β-lactamases has been found in Proteus infections. 30 Phenotypic methods could be implemented in routine diagnostic laboratories along with susceptibility testing, to help in the control of infections. 31 The low resistance levels observed in the present study might be associated with the relative inaccessibility and high prices of these drugs compared with other antimicrobials.…”

Section: Discussionmentioning

confidence: 99%

Spectrum of uropathogens and their susceptibility to antimicrobials in pregnant women: a retrospective analysis of 5-year hospital data

et al. 2021

View full text Add to dashboard Cite

Objective Urinary tract infections (UTI) are common during pregnancy. Identification of antimicrobial susceptibility patterns of microorganisms in pregnant women is important to select the most appropriate antimicrobial. We assessed common uropathogens in pregnant women with UTI and antimicrobial susceptibility, to guide empirical antibiotic selection. Methods In this retrospective study, we analyzed mid-stream urine culture and antibiotic susceptibility data from pregnant women who attended Jordan University Hospital during 2014 to 2018. Data were collected from patients’ charts and urine cultures, and sensitivity results were extracted from the laboratory electronic system. We calculated descriptive statistics and determined correlations among pathogens and antibiotics. Results We examined 612 positive urine cultures from 559 pregnant women, including 163 (29.2%) inpatients. Escherichia coli (29.4%) was the most frequently identified microorganism, followed by coagulase-negative staphylococci (CoNS) (21.6%). All bacterial isolates were sensitive to aztreonam, chloramphenicol, fosfomycin, ofloxacin, pefloxacin, piperacillin, and colistin sulfate; 87.5% were sensitive to amikacin. Only 15.79%, 18.93%, and 17.91% were sensitive to oxacillin, nalidixic acid, and erythromycin, respectively. Conclusion E. coli and CoNS were the most commonly identified microorganisms in this study. We found increased antibiotic resistance in Enterobacter species. The chosen antimicrobial therapy in pregnancy should be determined by sensitivity/resistance and fetomaternal safety.

show abstract

“…The presence of P. mirabilis carrying bla GES-1 is worrying because this gene encodes the ESBL enzyme and shows resistance to a wide range of β-lactams, such as resistance to 3rd generation (ceftazidime, cefotaxime, cefoxitin) and 4th generation cephalosporins (cefepime) and monobactams (Aztreonam) 13 . In addition, within the Proteus genus, there are no reports of this pathogen carrying bla GES .…”

Section: Discussionmentioning

confidence: 99%

First report of bla GES-1 in Proteus mirabilis clinical isolates

Beltrão

Oliveira

Lópes

2021

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

Proteus mirabilis is one of the main pathogens causing urinary tract infections and sepsis. To our knowledge, this is the first report of a P. mirabilis hosting bla GES . The presence of these genes was determined using PCR and sequencing. We identified the presence of bla GES-1 in all three isolates. In addition, we identified the bla KPC-2 and bla NDM-1 genes in the two strains. These data emphasize the importance of monitoring and surveillance of all enterobacteria. The circulation of P. mirabilis strains carrying bla GES-1 constitutes a new scenario of resistance in this species and should be an epidemiological alert for global health.

show abstract

Phenotypic detection and antibiogram of β-lactamase-producing proteus species in a tertiary care hospital, India

Cited by 21 publications

References 12 publications

Antimicrobial and Antibiofilm Activities of Probiotic Lactobacilli on Antibiotic-Resistant Proteus mirabilis

Antimicrobial and Antibiofilm Activities of Probiotic Lactobacilli on Antibiotic-Resistant Proteus mirabilis

Spectrum of uropathogens and their susceptibility to antimicrobials in pregnant women: a retrospective analysis of 5-year hospital data

First report of bla GES-1 in Proteus mirabilis clinical isolates

Contact Info

Product

Resources

About