Phenotypic detection of Extended Spectrum Beta- Lactamase producing uropathogens using DDST, PCT, Chrom agar and E-test – A comparitive study

Prabha, Ratna; Easow, Joshy M; Swapna, M.

doi:10.20546/ijcmas.2016.504.064

Cited by 7 publications

(6 citation statements)

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“…The Etest can confirm or detect a specific resistance phenotype like extended spectrum beta-lactamases (ESBL) producers. 28 This technique has shown a good correlation between the MIC values determined by Etest and those obtained by broth dilution or agar dilution methods. 29 , 30 The Etest is simple to implement and can be performed to investigate the antimicrobial interaction between 2 drugs, 31 which is why it is routinely requested by clinicians.…”

Section: Discussionmentioning

confidence: 91%

Frequency of Uropathogens and Antimicrobial Susceptibility in Childhood Urinary Tract Infection at Kamenge University Hospital, Bujumbura, Burundi

Nyandwi

Manirakiza²,

Ndirahisha³

et al. 2017

East African Health Res J

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Background: Increasing resistance to antimicrobials is a worldwide problem. The aim of our study was to determine the pathogens and antimicrobial susceptibility of bacteria causing urinary tract infection (UTI) in children. Methods: This is a prospective cohort study conducted over a 10-month period with 101 children hospitalised at Kamenge University Hospital for acute UTI. The infections were confirmed by Kass urinalysis criteria, and culture and susceptibility antibiotic tests were performed for isolated microbial agents. Results: Frequency of UTI in the overall population of children hospitalised at Kamange University Hospital was 8.4%. Of the 101 children with UTIs, 87 (86.1%) were under the age of 24 months. Diagnosis of pyelonephritis (82%) was the most common, followed by cystitis (18%). Escherichia coli (82%) was the most frequent pathogen causing UTI. We found E coli and Klebsiella pneumonia to be resistant to aminopenicillins (100%), cotrimoxazole (98.2%, 100%), Augmentin (amoxicillin/clavulanic acid) (70.5%, 80%), cefotaxime (45.8%, 28.6%), cefuroxime (36.8 to 45.5%, 50%), fluoroquinolones (33.3 to 53.6%, 28.6 to 50%), gentamicin (27.5%, 20%), and nitrofurantoin (9.3%, 50%). Conclusion: E coli is the main causal agent of UTI in childhood with a high resistance to antibiotics. Appropriate antibiotics for empiric therapy should be based on local circulating bacterial strains and resistance profiles.

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Section: Discussionmentioning

confidence: 91%

Frequency of Uropathogens and Antimicrobial Susceptibility in Childhood Urinary Tract Infection at Kamenge University Hospital, Bujumbura, Burundi

Nyandwi

Manirakiza²,

Ndirahisha³

et al. 2017

East African Health Res J

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“…These enzymes are plasmid-mediated and are derived from point mutation of TEM on SHV β-lactamases 8 . Detection of ESBL producers and other acquired resistance mechanisms to beta-lactam antibiotics by phenotypic methods are very important to prevent spread to other patients in hospitals by implementing isolation procedures 9 . Recently, isolates from community acquired infections are reported more commonly than isolates from hospital acquired infections 10 .…”

Section: Introductionmentioning

confidence: 99%

ESBL Producers in Gram Negative Isolates from Clinical Samples

Wadekar¹,

Sathish²,

Pooja³

et al. 2020

J Pure Appl Microbiol

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Resistance to beta lactam antibiotics is the most common cause for beta-lactamase production. Increasing number of extended spectrum beta-lactamase (ESBL) producers has reduced the treatment options which resulted in emergence of multidrug resistant strains, treatment failure and hence increased mortality. To detect phenotypically, ESBL producers in Gram negative isolates from different samples and to know their susceptibility pattern. A retrospective study of Gram negative isolates was conducted. Total of 521 isolates were isolated from various samples. They were processed and identified by standard procedures. The antibiotic susceptibility testing was performed by Kirby- Bauer disc diffusion method using CLSI guidelines. ESBL was detected by combination disk test. A total of 521 Gram negative isolates were isolated which included E. coli, Klebsiella pneumoniae, Citrobacter spp., Enterobacter spp., Proteus spp. and Acinetobacter spp. Pseudomonas aeruginosa. Of 521 isolates tested, ESBL was detected in 329 (63.1%) isolates. These isolates showed maximum susceptibility to piperacillin- tazobactam (86%) followed by imipenem (78.4%), amikacin (63.5%), cotrimoxazole (54.4%), ciprofloxacin (51%), amoxi-clav (44.9%), cefepime (44.1%), gentamicin (38.9%), cefoxitin (34.9%) and ampicillin (19.1%). ESBL producers which are resistant to beta lactam antibiotics have become a major problem. Detection of these beta-lactamase enzymes by simple disk method and its reporting will help clinicians in prescribing proper antibiotics.

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“…Urinary tract infections (UTI), also known as acute cystitis or bladder infection are a serious threat to human health affecting millions of people annually resulting in high morbidity and mortality, UTI is common in both males and females, females are more susceptible than males, with three-quarters of UTI occurring in pregnant women and one quarter in nonpregnant women. It is the most common form of bacterial infection in women with more than 10% developing UTI every year [1].…”

Section: Introductionmentioning

confidence: 99%

“…The irrational and inappropriate use of betalactam drugs has led to the advent of resistant strains worldwide. Beta-lactam resistance is mainly attributed to acquiring beta-lactamase genes localized on mobile circular genetic elements such as plasmids or transposons [1,3].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Phenotypic Characterization of Extended Spectrum Beta-lactamase Producing Bacteria from Urinary Tract Infection Patients in Adamawa State, Nigeria

Abdullahi

Marvin

2020

JAMMR

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Introduction: The irrational and inappropriate use of beta-lactam antimicrobial drugs has led to the advent of resistant strains worldwide. Beta-lactam resistance is mainly attributed to acquiring beta-lactamase genes, the consequence has been preventable treatment failures (sometimes fatal) in patients. Aims: To Identify the Incidence of Extended-spectrum Beta-lactamae Producing Bacteria In Urinary Tract Infection (U.T.I). Patients (outpatients and inpatients) of Specialist Hospital Jimeta-Yola Adamawa State, North-east Nigeria. Methodology: It was a Cross-sectional study carried out in Microbiology Unit of the Department of Science Laboratory Technology, School of Life Sciences, Modibbo Adama University of Technology Yola, Adamawa State, Nigeria. Between April and June 2016, The Strains Were Isolated, Identified And Screened For E.S.B.L By Double Disc Diffusion Synergy Tests (DDDST) Using Augmentin and Third-Generation Cephalosporin Cefuroxime-CRX, Cefixime-CXM and Ceftazidime-CAZ Purchased from Rapid labs. U.K. Results: Of the 150 isolates, 49%(74/150) were Gramm positive and 51%(76/150) were Gramm negative, S. aureus 29% (44/150) was found to be the most prevalent organism followed by E. coli 21%(31/150) while P. aeruginosa has the lowest 2%(4/150), of the 150 isolates,17.4%(26/150) were E.S.B.L producers with female patients accounting for the 84.6%(22/26), out of 31 E. coli isolates 25%(8/31) were ESBL producers, out of the 21 K. pneumonia isolates 19%(4/21) is ESBL producers, out of the 16 S. feacalis isolates 6%(1/16) is ESBL producers, out of the 14 P. mirabilis isolates 21%(3/14) is ESBL producers, out of the 4 isolates 50%(2/4) are ESBL producers and none of the isolates of S. epidermis and E. aeruginosa was ESBL producer. Conclusion: Periodic Study Of E.S.B.L Should Be Employed To Monitor Local Resistance Pattern And Comparative Study Of Both The Phenotypic And Genotypic Detection Should Be Employed To Authenticate The Reliability Of The Phenotypic Detection Method(S).

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Phenotypic detection of Extended Spectrum Beta- Lactamase producing uropathogens using DDST, PCT, Chrom agar and E-test – A comparitive study

Cited by 7 publications

References 0 publications

Frequency of Uropathogens and Antimicrobial Susceptibility in Childhood Urinary Tract Infection at Kamenge University Hospital, Bujumbura, Burundi

Frequency of Uropathogens and Antimicrobial Susceptibility in Childhood Urinary Tract Infection at Kamenge University Hospital, Bujumbura, Burundi

ESBL Producers in Gram Negative Isolates from Clinical Samples

Isolation and Phenotypic Characterization of Extended Spectrum Beta-lactamase Producing Bacteria from Urinary Tract Infection Patients in Adamawa State, Nigeria

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