This work was aimed to evaluate genetic diversity among five local and six introduced apple cultivars in the germplasm which located in the South of Syria based on 29 morphological characters of leaf and fruit. The analysis of variance showed significant differences at P < 0.05 for all measured parameters, however the mean values of Limb length and width, fruit weight, length, and width indicated to a wide range of diversity between local and introduced cultivars, that the introduced cultivars have mostly the highest mean value while the local cultivars have the lowest mean values, that clearly found in the local apple cultivar Ksairi (K) which revealed the lowest mean value of fruit weight (FW), fruit length (FL) and fruit diameter (FD) in the comparison with all studied cultivars. Likewise, principal component analysis (PCA) was used and the first 4 principal components accounted for 76.4% of the total variance, with eigen values 29.2%, 18.8%, 16.9% and 11.5% respectively.