Phenotypic Evaluation of a Hybrid Diploid Blueberry Population for Plant Development and Fruit Quality Traits

Rowland, Lisa J.; Ogden, Elizabeth L.; Vinyard, Bryan T.

doi:10.3390/agronomy10081067

Cited by 6 publications

(18 citation statements)

References 33 publications

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“…A comparison of the genes associated with these SNPs to our results found that one of the genes associated with fruit size (gene.g14573.t1, hypersensitive-induced response protein 1-SPFH/Band 7/PHB domain) is located in the vicinity of a minor QTL for 3 mm Firm from our study. Interestingly, we previously found that 20S Firm was moderately correlated with fruit size (Weight and Dia) in our diploid mapping population (Rowland et al, 2020). In addition, two of the genes associated with flower bud density (CUFF13871.1 and 54064_g.1) in Ferrão et al (2018) are located in the region of a minor QTL for Dia from our results.…”

Section: Discussionsupporting

confidence: 68%

“…Fruit firmness was also evaluated in our population (Rowland et al, 2020), and a major QTL for firmness (using the 20 S Firm method) was identified on LG06 that was consistent over 3 www.Vaccinium.org 2 years. By scanning through the list of annotated genes from the diploid genome in the 20 S Firm QTL region, we identified several genes encoding alpha-glucosidases and one encoding pectin methylesterase and pectin methylesterase inhibitor.…”

Section: Discussionmentioning

confidence: 99%

“…Traits included chilling requirement, cold hardiness, timing of various stages of development (flower bud, leaf bud, and fruit development), and various fruit quality traits (weight, diameter, color, scar size, firmness, flavor, and soluble solids). Phenotypic data collection, distribution, correlation, and heritability analyses were described in detail previously (Rowland et al, 2014(Rowland et al, , 2020. Briefly, all phenotypic evaluations used a completely randomized experimental design.…”

Section: Plant Materials and Phenotypic Evaluationmentioning

confidence: 99%

“…The day of the year (Julian day) when > 50% of buds reached each stage was recorded for each genotype. These 1 https://www.Canr.msu.edu/blueberries/growing_blueberries/growthstages developmental/growth stage traits were evaluated in 2012 and 2013 (Rowland et al, 2020).…”

Section: Plant Materials and Phenotypic Evaluationmentioning

confidence: 99%

“…For many years, our laboratory has been working on understanding the genetic control of important horticultural traits in blueberry breeding (Rowland et al, 1999). We have previously constructed a relatively low-density molecular marker-based genetic map of a diploid blueberry biparental population that segregates for chilling requirement, cold hardiness, and many fruit quality and plant development traits (Rowland et al, 2014(Rowland et al, , 2020. We constructed this pseudo-backcross interspecific mapping population by crossing a hybrid (F 1 #10) of a low-chilling, freezing-sensitive V. darrowii selection Fla4B and a high-chilling, freezing tolerant diploid V. corymbosum selection W85-20, to another diploid V. corymbosum selection W85-23.…”

Section: Introductionmentioning

confidence: 99%

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High-Density Linkage Map Construction and QTL Identification in a Diploid Blueberry Mapping Population

Ogden

Bostan

et al. 2021

Front. Plant Sci.

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Genotyping by sequencing approaches have been widely applied in major crops and are now being used in horticultural crops like berries and fruit trees. As the original and largest producer of cultivated blueberry, the United States maintains the most diverse blueberry germplasm resources comprised of many species of different ploidy levels. We previously constructed an interspecific mapping population of diploid blueberry by crossing the parent F1#10 (Vaccinium darrowii Fla4B × diploid V. corymbosum W85–20) with the parent W85–23 (diploid V. corymbosum). Employing the Capture-Seq technology developed by RAPiD Genomics, with an emphasis on probes designed in predicted gene regions, 117 F1 progeny, the two parents, and two grandparents of this population were sequenced, yielding 131.7 Gbp clean sequenced reads. A total of 160,535 single nucleotide polymorphisms (SNPs), referenced to 4,522 blueberry genome sequence scaffolds, were identified and subjected to a parent-dependent sliding window approach to further genotype the population. Recombination breakpoints were determined and marker bins were deduced to construct a high density linkage map. Twelve blueberry linkage groups (LGs) consisting of 17,486 SNP markers were obtained, spanning a total genetic distance of 1,539.4 cM. Among 18 horticultural traits phenotyped in this population, quantitative trait loci (QTLs) that were significant over at least 2 years were identified for chilling requirement, cold hardiness, and fruit quality traits of color, scar size, and firmness. Interestingly, in 1 year, a QTL associated with timing of early bloom, full bloom, petal fall, and early green fruit was identified in the same region harboring the major QTL for chilling requirement. In summary, we report here the first high density bin map of a diploid blueberry mapping population and the identification of several horticulturally important QTLs.

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Section: Discussionsupporting

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Section: Plant Materials and Phenotypic Evaluationmentioning

confidence: 99%

Section: Plant Materials and Phenotypic Evaluationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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High-Density Linkage Map Construction and QTL Identification in a Diploid Blueberry Mapping Population

Ogden

Bostan

et al. 2021

Front. Plant Sci.

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High-density linkage map construction and identification of loci regulating fruit quality traits in blueberry

et al. 2021

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Fruit quality traits play a significant role in consumer preferences and consumption in blueberry (Vaccinium corymbosum L). The objectives of this study were to construct a high-density linkage map and to identify the underlying genetic basis of fruit quality traits in blueberry. A total of 287 F1 individuals derived from a cross between two southern highbush blueberry cultivars, ‘Reveille’ and ‘Arlen’, were phenotyped over three years (2016–2018) for fruit quality-related traits, including titratable acidity, pH, total soluble solids, and fruit weight. A high-density linkage map was constructed using 17k single nucleotide polymorphisms markers. The linkage map spanned a total of 1397 cM with an average inter-loci distance of 0.08 cM. The quantitative trait loci interval mapping based on the hidden Markov model identified 18 loci for fruit quality traits, including seven loci for fruit weight, three loci for titratable acidity, five loci for pH, and three loci for total soluble solids. Ten of these loci were detected in more than one year. These loci explained phenotypic variance ranging from 7 to 28% for titratable acidity and total soluble solid, and 8–13% for pH. However, the loci identified for fruit weight did not explain more than 10% of the phenotypic variance. We also reported the association between fruit quality traits and metabolites detected by Proton nuclear magnetic resonance analysis directly responsible for these fruit quality traits. Organic acids, citric acid, and quinic acid were significantly (P < 0.05) and positively correlated with titratable acidity. Sugar molecules showed a strong and positive correlation with total soluble solids. Overall, the study dissected the genetic basis of fruit quality traits and established an association between these fruit quality traits and metabolites.

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There and back again; historical perspective and future directions for Vaccinium breeding and research studies

Edger

Iorizzo

Bassil

et al. 2022

Horticulture Research

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The genus Vaccinium L. (Ericaceae) contains a wide diversity of culturally and economically important berry crop species. Consumer demand and scientific research in blueberry (Vaccinium spp.) and cranberry (Vaccinium macrocarpon) have increased worldwide over the crops’ relatively short domestication history (~100 years). Other species, including bilberry (Vaccinium myrtillus), lingonberry (Vaccinium vitis-idaea), and ohelo berry (Vaccinium reticulatum) are largely still harvested from the wild but with crop improvement efforts underway. Here, we present a review article on these Vaccinium berry crops on topics that span taxonomy to genetics and genomics to breeding. We highlight the accomplishments made thus far for each of these crops, along their journey from the wild, and propose research areas and questions that will require investments by the community over the coming decades to guide future crop improvement efforts. New tools and resources are needed to underpin the development of superior cultivars that are not only more resilient to various environmental stresses and higher yielding, but also produce fruit that continue to meet a variety of consumer preferences, including fruit quality and health related traits.

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Phenotypic Evaluation of a Hybrid Diploid Blueberry Population for Plant Development and Fruit Quality Traits

Cited by 6 publications

References 33 publications

High-Density Linkage Map Construction and QTL Identification in a Diploid Blueberry Mapping Population

High-Density Linkage Map Construction and QTL Identification in a Diploid Blueberry Mapping Population

High-density linkage map construction and identification of loci regulating fruit quality traits in blueberry

There and back again; historical perspective and future directions for Vaccinium breeding and research studies

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