Phenotypic evaluation of acapsular transposon mutants of Vibrio vulnificus

Wright, Anita C.; Simpson, Linda M.; Oliver, James D.; Morris, J. Glenn

doi:10.1128/iai.58.6.1769-1773.1990

Cited by 194 publications

(110 citation statements)

References 25 publications

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“…Using a mouse model, they found a 100% correlation between virulence and colony morphology, with the cultures composed solely of translucent colonies being avirulent. Wright et al (1990) verified that the loss of capsule was accompanied by the loss of virulence. Recently, Harris-Young et al (1993 reported that opaque strains of V. vulnificus were more resistant to the bactericidal effect of haemocytes, through unknown mechanisms.…”

Section: Table 4 Number (%) Of Virulent and Avirulent Vibrio Vulnificmentioning

confidence: 80%

“…© 1998 The Society for Applied Microbiology al. 1986) ; ability to adhere to the surface of epithelial cells (Reyes et al 1985) ; presence of pili (Gander and LaRocco 1989) and ability to utilize transferrin-bound iron (Wright et al 1990). So far, however, the most critical element of V. vulnificus virulence, according to Simpson et al (1987), is encapsulation.…”

Section: Introductionmentioning

confidence: 99%

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Virulence factors and pathogenicity of Vibrio vulnificus strains isolated from seafood

Moreno

Landgraf

1998

J Appl Microbiol

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M . L . G A RC IA M OR EN O AN D M . LA ND G RA F. 1998. The virulence factors of Vibrio vulnificusare not yet well understood. So far, many hydrolytic enzymes have been implicated in the pathogenesis of this micro-organism. The present research was carried out in order to study the presence of some of these enzymes in 133 V. vulnificus strains isolated from 45 seafood samples. The results showed that 100% of these strains were positive for the production of lecithinase and lipase (Tween-80), 99·2% for caseinolytic protease, 96·9% for DNase, 65·4% for mucinase and 46·6% for elastase. None of the strains was positive for the production of collagenase and 96% were haemolytic against sheep blood cells. In relation to colony morphology on brain heart infusion (BHI) agar and nutrient agar, 59·4% of strains showed opaque morphology on BHI agar and 57·9% on nutrient agar, 10·5% presented translucent morphology on both agars and 30·1 and 31·6% of strains showed a mixture of opaque and translucent morphology on BHI agar and nutrient agar, respectively. None of the translucent colonies was virulent to mice. Therefore, opacity was a useful marker for potential virulence. Of 45 food samples contaminated with V. vulnificus, 29 (64·4%) presented strains lethal to adult mice.

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Section: Table 4 Number (%) Of Virulent and Avirulent Vibrio Vulnificmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Virulence factors and pathogenicity of Vibrio vulnificus strains isolated from seafood

Moreno

Landgraf

1998

J Appl Microbiol

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“…Several potential virulence factors have been suggested to be associated with V. vulnificus infection [3,4]. Of these, to date, only the capsule [5] and iron-sequestering system [6][7][8][9][10][11][12][13][14][15][16][17][18] have been confirmed to be authentic virulence factors, fulfilling the molecular version of KochÕs postulates. 0378 Many researchers have reported that iron plays a critical role in the pathogenesis of V. vulnificus septicemia [19][20][21][22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Vibrio vulnificusmetalloprotease VvpE has no direct effect on the iron-assimilation from human holotransferrin

Shin

Sun

Park

et al. 2005

FEMS Microbiology Letters

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In order to elucidate the role of Vibrio vulnificus metalloprotease VvpE in the uptake of iron from human transferrin, we constructed a VvpE-deficient mutant and a merozygotic vvpE-transcriptional reporter from the wild type strain MO6-24/O. All three strains were able to grow only in deferrated Heart Infusion broth (DF-HI) with human holotransferrin (HT), but not in DF-HI containing partially iron-saturated transferrin or apotransferrin, without noticeable differences among the strains. All strains consumed most iron in the early growth phase. Both the transcription and extracellular production of VvpE proceeded at undetectable levels when bacterial growth was severely retarded in the DF-HI. When HT or FeCl(3) was added to the DF-HI, the retarded bacterial growth was restored and vvpE transcription dramatically increased in the late growth phase, but the extracellular VvpE production was negligible as compared to its transcription. All strains were unable to degrade HT even in normal HI broth containing HT, in which extracellular VvpE activity was remarkably high. The uptake of iron from HT in all strains was consistent with the production of catechol-siderophore rather than hydroxamate-siderophore. Similar results were also observed when clinical isolates from septicemic patients were used. In conclusion, we determined that VvpE was not directly involved in the siderophore-mediated iron-uptake from human transferrin. In addition, the discrepancy between the transcription and extracellular production of VvpE suggests that additional posttranscriptional events are involved in the extracellular production of VvpE.

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“…Both fructose and mannose inhibited adherence for all isolates tested, although the extent of the inhibition varied with both sugar and isolate. Colony opacity, which is indicative of the presence of capsule (Wright et al 1990), did not correlate with adherence to buccal cells, hydrocarbons or inhibition of epithelial cell adherence by a particular sugar (mannose or fructose).…”

Section: Adherence To Epithelial Cellsmentioning

confidence: 84%

Surface properties of Vibrio vulnificus

Plotkin

Konaklieva

2007

Lett Appl Microbiol

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Aims: Vibrio vulnificus adheres to a diverse range of surfaces, ranging from the chitinous exoskeleton of mollusks to human tissue. To determine whether environmental and human clinical isolates exhibit different adhesion traits, we studied the ability of 10 environmental isolates and 10 clinical isolates to adhere to human epithelial cells and hydrocarbons with log P values ranging from 3·1 to 8·2. Methods and Results: All isolates adhered to varying levels to epithelial cells, and were inhibited to various extents from adherence by mannose and fructose. There was a lack of correlation between adherence to either hydrocarbons or cells and colony opacity. Adherence to hydrocarbons was optimal for solvents with a log P < 8·2. Conclusions: Vibrio vulnificus clinical and environmental isolates exhibit differential adherence to epithelial cells and hydrocarbons. Significance and Impact of the Study: The differential adherence of organisms to hydrocarbons based on log P may have utility in drug design and enhancement of food safety.

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Phenotypic evaluation of acapsular transposon mutants of Vibrio vulnificus

Cited by 194 publications

References 25 publications

Virulence factors and pathogenicity of Vibrio vulnificus strains isolated from seafood

Virulence factors and pathogenicity of Vibrio vulnificus strains isolated from seafood

Vibrio vulnificusmetalloprotease VvpE has no direct effect on the iron-assimilation from human holotransferrin

Surface properties of Vibrio vulnificus

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