Phenotypic evaluation of genetic resistance to the tick Rhipicephalus (Boophilus) microplus in Argentine Creole cattle

Identification of Anaplasma marginale, Babesia bovis and Babesia bigemina resistance alleles in Crioulo Lageano cattle using PCR-SBT and BoLA-DRB3 gene sequencing

“…In addition to greater genetic diversity when compared to commercial breeds, native breeds are adapted to the specific environmental conditions of their region after than more than five centuries of natural selection and, in general, there are reports of great resistance to local pathogens ( 22 ). In Argentina, Argentine Creole cattle, also Bos taurus , demonstrated phenotypic resistance to Riphicephalus (Boophilus) microplus tick infestation in experimental infection ( 36 ). Tick resistance may also be a factor that can influence resistance to hemoparasites.…”

Deciphering the genetic battle: How Argentine Creole cattle challenge Rhipicephalus (Boophilus) microplus through their genome

“…For genotyped parent-offspring pairs, mismatches in pedigree errors were checked using the percentage of Mendelian con icts proposed by Wiggans et al ( 2009) using the seekparentf90 software from the BLUPF90 family of programs (Misztal et al 2002; http://nce.ads.uga.edu/wiki/doku.php). In the herd studied, the sampling size and the reduced genetic variation for naturally resistant in the Argentine Creole cattle (Ortega et al, 2023) made the h 2 estimation di cult. For this reason, in our work we used for the GWAS the additive and residual variance reported by Mapholi et al (2016; σ 2 a = 0.07, σ 2 e = 0.55, h 2 = 0.12) because both Nguni African and Argentine Creole breeds are native that are adapted to their hard environments, exhibiting high level of resistance to tick infestation (Spickett et al, 1989, Scholtz et al, 1991Mapholi et al, 2016;Ortega et al, 2023).…”

“…In the herd studied, the sampling size and the reduced genetic variation for naturally resistant in the Argentine Creole cattle (Ortega et al, 2023) made the h 2 estimation di cult. For this reason, in our work we used for the GWAS the additive and residual variance reported by Mapholi et al (2016; σ 2 a = 0.07, σ 2 e = 0.55, h 2 = 0.12) because both Nguni African and Argentine Creole breeds are native that are adapted to their hard environments, exhibiting high level of resistance to tick infestation (Spickett et al, 1989, Scholtz et al, 1991Mapholi et al, 2016;Ortega et al, 2023). Linkage disequilibrium (LD) between SNPs and linkage blocks were estimated using the --r2 --ld-window-r2 and -blocks commands implemented in the Plink 1.9 software (Purcell et al, 2007).…”

“…Taking into account its limited diffusion, due to the erroneous perception of being an unproductive breed, the Argentine Creole cattle can also be considered as a genetic reservoir of a species (Bos taurus) on which strong selection pressure has been exerted by man (Bouzat et al, 1998). In this sense, the reports of farmers qualify the breed as more resistant to diseases (Armstrong et al, 2022) included high levels of tick resistance infestation (Ortega et al, 2023).…”