Phenotypic flexibility in response to environmental salinity in the euryhaline crab Neohelice granulata from the mudflat and the saltmarsh of a SW coastal lagoon

Pinoni, Silvina Andrea; Michiels, María Soledad; Mañanes, Alejandra Antonia López

doi:10.1007/s00227-013-2258-9

Cited by 17 publications

(52 citation statements)

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“…The differences in the number of amylase forms present in the hepatopancreas of crustaceans have been associated with variations in environmental and/or physiological factors (Borowsky, 1984;Le Moullac et al, 1997;Perera et al, 2008aPerera et al, , 2008bCastro et al, 2012). N. granulata behaves as a hyper/hyporegulator since it exhibits hemolymph osmolarity values higher and lower than those of the corresponding external medium upon acclimation to 6-10 psu and 37-60 psu, respectively, while it osmoconforms at 35 psu (Asaro et al, 2011;Pinoni et al, 2013;Michiels et al, 2015a;Pinoni et al, 2015; this work, Table). The additional 30 kDa species found by zymogram analysis in the hepatopancreas from crabs acclimated to low salinity (10 psu) (hyperregulation conditions) (Figure 1b) suggests that mechanisms of modulation at the molecular level occur, which could lead to the higher total amylase activity detected by biochemical analysis.…”

Section: Discussionmentioning

confidence: 99%

Amylase in the hepatopancreas of a euryhaline burrowingcrab: characteristics and modulation

Asaro¹,

Paggi²,

Castro³

et al. 2017

Turk J Zool

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In spite of its inherent physiological importance, studies on the occurrence, characteristics, and modulation of amylase in euryhaline crabs are lacking. We investigated the occurrence of amylase forms and the effect of acclimation to different salinities on their number and made a partial purification and characterization of the major form present in the hepatopancreas of Neohelice granulata. Zymogram analysis revealed 5 amylase forms in crabs acclimated to 35 psu (seawater) and 37 psu, and an additional band at 10 psu, but with a major form (29 kDa) in all cases, which was partially purified and characterized. Amylolytic activity was maximal between 30 and 40 °C; maintained at high NaCl concentrations (up to 4 M); increased by 5 mM K + , Li + , Co 2+ , and Mg 2+ (36%-45%); inhibited by Cu 2+ , Zn 2+ , Cd 2+ , Fe 2+ , and Mn 2+ (92.4% and 23.7%); not affected by Ni 2+ or Ba 2+ ; and enhanced almost 100% by Ca 2+ . Amylase exhibited Michaelis-Menten kinetics (starch: K m = 1.24 mg mL -1 ; glycogen: K m = 16.19 mg mL -1 ). The potential physiological significance and relationship to habitat conditions of the extra form in low salinity and the biochemical characteristics of the partially purified amylolytic activity (halotolerant, differential sensitivity to ions, capability to hydrolyze starch and glycogen) are discussed.

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Section: Discussionmentioning

confidence: 99%

Amylase in the hepatopancreas of a euryhaline burrowingcrab: characteristics and modulation

Asaro¹,

Paggi²,

Castro³

et al. 2017

Turk J Zool

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“…Phenotypic flexibility implies reversible modifications within individual phenotypic traits (from molecular to organisms) which can increase the chances of survival for animals facing changes in environmental conditions (Piersma & Drent, 2003; Pfenning et al , 2010; Kelly et al , 2012). The ability to osmoregulate is a primary physiological determinant for euryhalinity and for the occupancy of different zones within an intertidal area (Pinoni et al ., 2013; Larsen et al ., 2014; Havird et al ., 2016). Hyper- and hypo-osmoregulation abilities allow maintenance of the osmotic concentration of the haemolymph within a stable range, above or below that of the aquatic environment in low and high salinities, respectively (McNamara & Faria, 2012; Romano & Zeng, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Decapod crustaceans use carbohydrates, lipids and proteins as principal energy molecules but the extent of use and the relative importance of the different metabolic sources involved vary depending on species and type of challenge (Pinoni et al ., 2013; Jimenez & Kinsey, 2015; Wang et al ., 2016). In males of various species of decapod crustaceans, adaptation to environmental salinity is a complex process involving adjustments at the biochemical level in several tissues and organs and a metabolic reorganization (Michiels et al ., 2013, 2015; Pinoni et al ., 2013; Chen et al ., 2015). Modifications in various haemolymph metabolites have been reported (Romano et al ., 2012, 2014; Larsen et al ., 2014; Prymaczok et al ., 2016).…”

Section: Introductionmentioning

confidence: 99%

“…The hepatopancreas, due to its central role for storage of energy substrates and synthesis of macromolecules, is a sensitive indicator for metabolism modifications related to osmoregulatory processes (Zeng et al ., 2010; Ribeiro et al ., 2014; Wang et al ., 2014). Anterior and posterior gills also have a role as energy storage in various osmoregulating crabs although with differential degree of importance depending on species, type of energy substrate and salinity challenge (Pinoni et al ., 2013).…”

Section: Introductionmentioning

confidence: 99%

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The euryhaline crabUca tangerishowed metabolic differences to sex and environmental salinity

et al. 2017

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This study constitutes a first attempt to investigate sex differences in osmoregulatory capacity and metabolic responses in relation to hyper- and hypo-osmoregulation in the intertidal euryhaline crab Uca tangeri. Adult male and female specimens from Cadiz Bay, Spain (36°23′–37′N 6°8′–15′W), were acclimated to three different environmental salinities (12, 33 and 55 psu) during 7 days, and several parameters were assessed in haemolymph (osmolality, glucose, amino acids, triglycerides and lactate) as well as in metabolic key organs (hepatopancreas, anterior and posterior gills: glycogen, free glucose, amino acids and triglycerides). Specimens from both sex exhibited high and similar hyper- and hypo-osmoregulatory capacities. However, metabolite levels were differentially affected upon acclimation to low and high salinity in several metabolic organs and haemolymph of male and females: (i) glycogen in gills, (ii) free glucose in gills and hepatopancreas, (iii) amino acids in hepatopancreas, (iv) triglycerides in haemolymph, hepatopancreas and posterior gills, and (v) lactate in haemolymph. The results suggest the occurrence of differential metabolic adjustments upon hyper- and hypo-osmoregulation related to sex in the intertidal euryhaline crab U. tangeri.

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“…At low salinities, they hyper regulate actively by absorbing through their gills Na+ and Cl-from the environment (Kirschner). The muscle and the hepatopancreas take part in adjustment mechanisms subsidiary to osmo-ionoregulation (Li et al, 2008;Martins et al, 2011;Michiels et al, 2013;Pinoni et al, 2013;Longo & Díaz, 2015). For instance, the muscle has been involved in lipid mobilization under hyposmotic stress (Luvizotto-Santos et al, 2003) and in the differential response of diverse enzymes in osmoregulatory and osmoconformatory conditions (Pinoni & López Mañanes, 2008;Pinoni & López Mañanes, 2009).…”

Section: Introductionmentioning

confidence: 99%

Influence of Reduced Salinity on the Histochemical Fiber Type Composition of the Claw Closer Muscle of Two Estuarine Crab Species, Cyrtograpsus angulatus and Neohelice granulata (Decapoda: Varunidae)

Longo

Díaz

2017

Int. J. Morphol.

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The estuarine crustaceans are exposed to frequent and abrupt environmental salinity changes that involve adjustments at different levels in muscle tissue. The histochemical composition of fiber types of the claw closer muscle of Cyrtograpsus angulatus and Neohelice granulata (Dana, 1851) under hyper regulatory conditions was analyzed. Cryosections of the muscle were treated with myosin-adenosine triphosphatase (m-ATPase), succinic dehydrogenase (SDH), periodic acid Schiff (PAS) and Sudan Black B. The mean diameters, the relative area and the proportion of each muscle fiber type were calculated. Types I and IV would belong to 'extreme' groups, whereas types II and III would be considered 'intermediate'. Type I fibers were large and exhibited a weak reaction to all techniques; type IV fibers were small and reacted strongly to histochemical tests. Types II and III prevailed in C. angulatus whereas type II predominated in N. granulata. Type IV fibers were absent in C. angulatus and scarce in N. granulata. The claw closer muscle of N. granulata and C. angulatus exhibited differential responses under reduced salinity at a histochemical level. Therefore, the existence of different adjustment mechanisms facing salinity stress is suggested.

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Phenotypic flexibility in response to environmental salinity in the euryhaline crab Neohelice granulata from the mudflat and the saltmarsh of a SW coastal lagoon

Cited by 17 publications

References 68 publications

Amylase in the hepatopancreas of a euryhaline burrowingcrab: characteristics and modulation

Amylase in the hepatopancreas of a euryhaline burrowingcrab: characteristics and modulation

The euryhaline crabUca tangerishowed metabolic differences to sex and environmental salinity

Influence of Reduced Salinity on the Histochemical Fiber Type Composition of the Claw Closer Muscle of Two Estuarine Crab Species, Cyrtograpsus angulatus and Neohelice granulata (Decapoda: Varunidae)

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