Phenotypic indications that human sweat glands are a rich source of nestin-positive stem cell populations

Petschnik, Anna Emilia; Klatte, Jennifer E.; Evers, L.H.; Kruse, Charli; Paus, Ralf; Danner, Sandra

doi:10.1111/j.1365-2133.2009.09512.x

Cited by 33 publications

(30 citation statements)

References 15 publications

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“…[17][18][19][20] Recently, several studies demonstrated similar nestinpositive stem cell populations present mainly in the follicular bulge and connective tissue sheath, human sweat glands, as well as randomly distributed in small numbers throughout the interfollicular dermis. 1,2,5,[20][21][22][23][24][25][26] In cutaneous malignancies, studies have demonstrated up-regulated nestin expression in melanomas, cutaneous peripheral nerve sheath tumours such as malignant peripheral nerve sheath tumour, and also mesenchymal tumours such as dermatofibrosarcoma protuberans. 4,[27][28][29] Although few studies have examined the expression of CK15 and CK19 in basal cell carcinoma (BCC) and SCC, only rarely have nestin expression been examined in the two most common cutaneous malignancies.…”

Section: Introductionmentioning

confidence: 99%

Expression of stem cell markers nestin and cytokeratin 15 and 19 in cutaneous malignancies

Abbas

Bhawan

2011

Acad Dermatol Venereol

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Section: Introductionmentioning

confidence: 99%

Expression of stem cell markers nestin and cytokeratin 15 and 19 in cutaneous malignancies

Abbas

Bhawan

2011

Acad Dermatol Venereol

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“…In Fig. 5 hSGSCs are stained for Nestin, which is expressed by more than 80 % of cells in an hSGSCs culture [28]. Neurites, marked with β3-Tubulin, aligned with the orientation of hSGSCs.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, hSGSCs represent a promising source of autologous stem cells for cell replacement therapies. A biopsy of human axillary skin is sufficient and avoids any surgical intervention [28]. In an in vivo full-thickness skin defect, hSGSCs were able to improve vascularisation during scaffold-dependent dermal regeneration [22].…”

Section: Discussionmentioning

confidence: 99%

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Co-Culture Systems of Human Sweat Gland Derived Stem Cells and Peripheral Nerve Cells: Anin VitroApproach for Peripheral Nerve Regeneration

Mehnert

Kisch

Brandenburger

2014

Cell Physiol Biochem

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Background/Aims: The treatment of peripheral nerve lesions still represents a clinical challenge. Several approaches such as novel biomaterials for nerve guides, addition of growth factors or cellular supplements moved in the focus of research. Especially the application of autologous stem cells is highly promising for future applications. Human sweat gland derived stem cells (hSGSCs) represent an easy accessible source of autologous adult stem cells and did already show a beneficial effect in dermal wound healing. Methods: In this study, the effect of hSGSCs on neurite outgrowth of primary adult or prenatal Dorsal root ganglia (DRG) neurons was analysed in an indirect co-culture model. Additionally, direct co-cultures with hSGSCs as a feeder layer were performed. Results: Adult and prenatal DRG neurons showed increased neurite outgrowth after 24 h co-culture with hSGSCs. The outgrowth increased significantly by the factors 5.6 and 2.6 respectively. Direct co-cultures revealed neurite alignment along the hSGSCs orientation. Conclusion: The paracrine influence of hSGSCs on neurite outgrowth, but also their ability to operate as a feeder layer with guidance properties shows great potential for future applications in peripheral nerve regeneration.

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“…Hence, we applied isolation procedures and propagation methods for the harvesting of mammary stem cells similar to those we have already established for pancreatic, submandibular or sweat gland-derived stem cells [26,29,33]. We opted to isolate stem cells from the mammary tissue of female and male donors via adherence to conventional cell culture plastic, similar to the methods we used for the other glandular stem cells and also similarly applied by Cregan and colleagues for breast milk-derived stem cells [21].…”

Section: Introductionmentioning

confidence: 99%

Mammary gland-derived nestin-positive cell populations can be isolated from human male and female donors

Richter

Nissen

Mailänder³

et al. 2013

Stem Cell Res Ther

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IntroductionNestin-expressing cells isolated from different human tissues reveal self-renewal capacity and a multilineage differentiation potential. In particular, adult stem/progenitor cell populations from exocrine glands such as the pancreas, salivary gland and sweat gland are characterized by prominent nestin expression. Interestingly, human mammary gland histological examinations also demonstrated the existence of nestin-positive cells in the ductal compartments. Within the scope of our previous work we wonder whether an isolation of nestin-positive cell populations from human mammary gland biopsies is possible and what characteristics they have in vitro. Cell populations from both sexes were propagated and subjected to a comparison with other gland-derived cell populations.MethodsHuman mammary tissue biopsies were mechanically and enzymatically treated, and the isolated acini structures were observed with time-lapse microscopy to track adherently outgrowing cells. The proliferation potential of the cell population was assessed by performing growth curves. On the gene and protein levels we investigated the expression of stem cell markers as well as markers indicating multilineage differentiation.ResultsWe succeeded in establishing proliferating cell populations from breast tissue biopsies of both sexes. Our results display several similarities to the glandular stem cell populations from other exocrine glands. Beside their proliferation capacity during in vitro culture, the obtained cell populations are characterized by their prominent nestin expression. The cells share surface proteins commonly expressed on adult stem cells. We demonstrated the expression of stem cell-related genes like Oct4, Sox2, KLF4 and Nanog, and confirmed multipotent differentiation capacity by detecting transcripts expressed in endodermal, mesodermal and ectodermal cell types.ConclusionWith this study we present an efficient procedure for isolation and propagation of nestin-positive stem cells obtained from male and female breast tissue, which is frequently available. The established multipotent cell populations could be easily expanded in vitro and thus hold promise for cell-based therapies and personalized medicine.

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Phenotypic indications that human sweat glands are a rich source of nestin-positive stem cell populations

Cited by 33 publications

References 15 publications

Expression of stem cell markers nestin and cytokeratin 15 and 19 in cutaneous malignancies

Expression of stem cell markers nestin and cytokeratin 15 and 19 in cutaneous malignancies

Co-Culture Systems of Human Sweat Gland Derived Stem Cells and Peripheral Nerve Cells: Anin VitroApproach for Peripheral Nerve Regeneration

Mammary gland-derived nestin-positive cell populations can be isolated from human male and female donors

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