Phenotypic Study of Peripheral Blood Lymphocytes and Humoral Immune Response in <i>Helicobacter pylori</i> Infection According to Age

Soares, Taciana F.; Rocha, Gifone Aguiar; Rocha, Andréia Maria Camargos; Corrêa-Oliveira, Rodrigo; Martins‐Filho, Olindo Assis; Carvalho, Anfrisina S. T.; Bittencourt, Paulo Fernando Souto; Oliveira, C. A.; Faria, Ana Maria Caetano; Queiroz, Dulciene Maria Magalhães

doi:10.1111/j.1365-3083.2005.01638.x

Cited by 17 publications

(27 citation statements)

References 34 publications

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“…In adults, H. pylori infection increases the number of CD4 + T-cells and induces activation of both CD4 + and CD8 + T-cells [6,[8][9][10]. Conversely, infection in children without DU affects neither the number of CD4 + and CD8 + T-cells nor the percentage of activation of the T-cells and co-stimulatory markers [6].…”

Section: Discussionmentioning

confidence: 99%

“…Activation and co-stimulatory markers were presented as a median percentage of CD4 + ⁄ CD28 + , CD4 + ⁄ HLA-DR + , CD8 + ⁄ CD28 + , CD8 + ⁄ HLA-DR + and CD19 + ⁄ CD5 + cells. The gastric inflammatory data were correlated with the absolute number ⁄ mm 3 of T-and B-cells, and with activation and co-stimulatory T-cell percentages, scored using a threepoint system based on the reference values for each cell population [6]. This system was as follows: for CD4 + ⁄ CD28 + : 0, <95%; 1, 95-96%; 2, 97-98%; and 3, >99% of the marked cells; for CD4 + ⁄ HLA-DR + : 0, <5%; 1, 5-10%; 2, 11-15%; and 3, >15% of the marked cells; for CD8 + ⁄ CD28 + : 0, <25%; 1, 25-50%; 2, 51-80%; and 3, >80% of the marked cells; and for CD8 + ⁄ HLA-DR + : 0, <20%; 1, 20-40%; 2, 41-70%; and 3, >70% of the marked cells.…”

Section: P a T I E N T S A N D M E T H O D Smentioning

confidence: 99%

“…Peripheral blood lymphocyte phenotypes were analysed by immunofluorescence as described previously [6]. The results were expressed as absolute numbers ⁄ mm 3 for CD4 + ⁄ CD3 + , CD8 + ⁄ CD3 + and CD19 + ⁄ CD3 -cell sets, based on the total number of peripheral blood lymphocytes, and as percentages for HLA-DR + -and CD28 + -expressing CD4 + and CD8 + T-cells (CD4 + HLA-DR + ⁄ CD4 + cells · 100; CD8 + HLA-DR + ⁄ CD8 + cells · 100; CD4 + CD28 + ⁄ CD4 + cells · 100; and CD8 + CD28 + ⁄ CD8 + cells · 100), and also for CD5 + -expressing CD19 + cells (CD19 + CD5 + ⁄ CD19 + cells · 100).…”

Section: P a T I E N T S A N D M E T H O D Smentioning

confidence: 99%

“…Recently, it has been demonstrated that H. pylori infection in children without DU, in contrast to adults, does not increase the number of CD4 + T-cells in the blood, or the percentage of activation and co-stimulatory T-cell markers [6]. Since the immune response may contribute to the outcome of H. pylori infection, the present study investigated CD4 + T-cells (CD4 + ⁄ CD3 + ) and CD8 + T-cells (CD8 + ⁄ CD3 + ), Bcells (CD19 + ⁄ CD3 -) and B1a-cells (CD19 + ⁄ CD5 + ), as well as cell activation (CD4 + ⁄ HLA-DR + and CD8 + ⁄ HLA-DR + ) and co-stimulatory (CD4 + ⁄ CD28 + and CD8 + ⁄ CD28 + ) markers, in peripheral blood from H. pylori-infected children with and without DU, and compared the results with those obtained from adults.…”

Section: Introductionmentioning

confidence: 98%

“…Although duodenal ulcer (DU) is less common in children, primary DU in childhood is associated frequently with H. pylori infection [4,5]. The reasons for the uncommon early onset of duodenal peptic ulcer are unknown, but may be associated with an immune response of the host linked to age [6], rather than with the shorter duration of the infection in childhood. Indeed, there are marked differences in the gastric inflammatory response to H. pylori infection between children and adults with DU [7].…”

Section: Introductionmentioning

confidence: 99%

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Differences in peripheral blood lymphocyte phenotypes between Helicobacter pylori-positive children and adults with duodenal ulcer

Soares

Rocha

et al. 2007

Clinical Microbiology and Infection

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The immunological mechanisms involved in the development of duodenal ulcer, especially in childhood, are unclear. Helicobacter pylori-positive children and adults, with and without duodenal ulcer, were therefore compared with respect to CD4(+) T-cells, and CD8(+) T-cells, B-cells and B1a-cells, as well as cell activation (CD4(+)/HLA-DR(+) and CD8(+)/HLA-DR(+)) and co-stimulatory (CD4(+)/CD28(+) and CD8(+)/CD28(+)) markers, in peripheral blood. Children with and without duodenal ulcer differed significantly. In particular, there was a phenotypic change in CD8(+) T-cells from children with ulcer that involved a 200% increase in the number of CD8(+)/HLA-DR(+) cells/mm(3) and a decrease of 34.2% in the number of CD8(+)/CD28(+) cells/mm(3). This phenotype of chronically activated memory CD8(+) T-cells, which has also been observed in patients with AIDS and tuberculosis, is associated with disease severity and progression. A lower frequency of B1a-cells was also observed in the group of children with ulcer. Conversely, no difference between infected adults with and without ulcer was observed, but the percentage of CD4(+)/HLA-DR(+) cells was lower in adults with ulcer, suggesting that a down-regulated immune response may play a role in the development of duodenal ulcer in adults. Gastric inflammation correlated positively with CD4(+) and chronically activated CD4(+) T-cells in children and adults without duodenal ulcer, respectively. These results suggest that there are differences in the immunophenotyping profile between H. pylori-positive children and adults with duodenal ulcer, indicating the possibility of distinct immune mechanisms in the development of the disease according to age.

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