Phenotypic traits, virulence-associated gene profile and genetic relatedness of <i>Edwardsiella tarda</i>
 isolates from Japanese eel <i>Anguilla japonica</i>
 in Korea

Kim, K.I.; Kang, JeongWoo; Park, J.Y.; Joh, Seong-Joon; Lee, H.S.; Kwon, Yong-Kuk

doi:10.1111/lam.12172

Cited by 17 publications

(24 citation statements)

References 26 publications

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“…The previous study showed that ank, katB and citC genes were present only in virulent strains of E. tarda (Nakamura et al, 2013;Srinivasa Rao et al, 2003). Kim et al, (2014) reported less distribution of ank and luxS genes in E. tarda isolated from Japanese eel in Korea, compared with other virulence genes including fimA, evpP, esa and astA. Our result, i.e.…”

Section: Discussionsupporting

confidence: 50%

“…The known 11 virulence-associated genes (ank, katB, luxS, citC, astA, evpP, mukF, gadB, fimA, hlyA, esa) of E. tarda were detected by PCR amplification using previously reported primers (Kim et al, 2014;Li, Ni, Liu, & Lu, 2011) and under the above-described conditions.…”

Section: Detection Of Virulence-associated Genesmentioning

confidence: 99%

“…However, the majority of the studies on E. tarda have examined either the antibiotic resistance (Lo, Lee, Wang, & Kuo, 2014;Wei et al, 2011) or the pathogenicity (Castro et al, 2016;Okuda et al, 2014;Srinivasa Rao et al, 2003) and thus very little information is currently available concerning both antibiotic resistance and pathogenicity of E. tarda strains (Kim et al, 2014;Yu et al, 2012). In this study, we investigated the antibiotic susceptibility, antibiotic resistance and virulence-associated gene profiles, phylogenetic diversity, and pathogenicity to zebrafish on E. tarda isolates derived from different cultured fish in Japan.…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, disease outbreaks caused by bacterial pathogens have affected fish farming all over the world. Edwardsiellosis caused by Edwarsiella tarda has been reported worldwide in economically important fish species, including Japanese eel Anguilla japonica, red seabream Pagrus major, olive flounder Paralichthys olivaceus, channel catfish Ictalurus punctatus, Asian catfish Clarias batrachus and turbot Scophthalmus maximus (Alcaide, Herraiz, & Esteve, 2006;Castro et al, 2016;Kim et al, 2014;Xu & Zhang, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…The pathogenic strains of E. tarda have virulence genes which might be absent in non-pathogenic strains (Castro et al, 2016;Srinivasa Rao et al, 2003). Now, many virulence-related genes in E. tarda have been cloned and reported, which include catalase (katB), type III secretion system (TTSS) (ast), TTSS regulator (esrB), type VI secretion system (T6SS) extracellular apparatus (evp), putative killing factor (mukF), fimbrial operon (fimA), glutamate decarboxylase (gadB), citrate lyaseligase (citC), hemolysins (hlyA), ankyrin like proteins (ank), and quorum sensing system (luxS) (Kim et al, 2014;Okuda, Takeuchi, & Nakai, 2014;Srinivasa Rao et al, 2003). Some studies have been conducted to evaluate the pathogenicity of E. tarda using the zebrafish Danio rerio as the animal model (Kim et al, 2014;Okuda et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Wimalasena¹,

Pathirana²,

Silva³

et al. 2019

Turk. J. Fish. Aquat. Sci.

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Edwardsiella tarda is one of the most significant bacterial pathogens to fish aquaculture worldwide. Here, we evaluated antibiotic resistance profiles, virulence genotypes and phylogenetic relationship of 30 Edwardsiella tarda isolates derived from cultured fish in Japan. The E. tarda isolates were phylogenetically (16S rRNA) clustered into two clads. The isolates of clad 1, which were mainly originated from olive flounder Paralichthys olivaceus and Japanese eel Anguilla japonica, had all of the known 11 virulence genes (ank, katB, LuxS, citC, astA, evpP, mukF, gadB, fimA, hlyA, esa) for E. tarda and mostly one or two of three aminoglycoside resistance genes, i.e. aac(6')-Ib, armA and strA-strB, if exists. In contrast, the isolates of clad 2, which were mainly originated from red seabream Pagrus major and black seabream Acanthopagrus schlegelii, lacked four (ank, katB, LuxS, citC) of the 11 virulence genes and, eight of them harbored mainly a β-lactam resistance gene (bla CTX-M). In infection experiments with zebrafish, representative isolates of the clad 1 showed higher virulence than those of the clad 2. These results reveal that the virulence profiles may be applicable for prediction of pathogenicity in fish. Phylogenetic analysis revealed that E. tarda isolates derived from olive flounder and red seabream are ancestor differentiated.

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Section: Discussionsupporting

confidence: 50%

Section: Detection Of Virulence-associated Genesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Untitled

Wimalasena¹,

Pathirana²,

Silva³

et al. 2019

Turk. J. Fish. Aquat. Sci.

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Morganellaceae , Erwiniaceae , Hafniaceae , and Selected Enterobacterales

Giffen,

Alby

2023

ClinMicroNow

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The reclassification of the order Enterobacteriales to Enterobacterales as well as the taxonomic shuffling of many members of the former family Enterobacteriaceae has increased the importance and awareness of a number of non‐ Enterobacteriaceae families. These include commonly known families, such as Morganellaceae , and lesser–known families, such as Budviciaceae . The family Erwiniaceae contains many important plant‐associated pathogens. Pantoea species have been isolated from plants, humans, and the natural environment, although they are most commonly plant pathogens. While stand‐alone nucleic acid amplification testing is uncommon for detection of Enterobacterales in clinical specimens, syndromic panel testing by nucleic acid amplification has allowed for the rapid progression of clinical microbiology diagnostics. The Morganellaceae are the most familiar organisms for which antimicrobial susceptibility testing is routinely performed. There are several FDA‐cleared panels available for a variety of clinical presentations, including positive blood cultures, pneumonia, and gastrointestinal infections. The GenMark panel includes Morganella morganii and specific identification of Proteus mirabilis .

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