Phenotypic, transcriptional, physiological and metabolic responses to carbon nanodot exposure in<i>Arabidopsis thaliana</i>(L.)

Chen, Jing; Liu, Baofeng; Yang, Zhongzhou; Qu, Jiao; Xun, Hongwei; Dou, Runzhi; Gao, Xiang; Wang, Li

doi:10.1039/c8en00674a

Cited by 39 publications

(33 citation statements)

References 64 publications

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“…The authors mostly noted the very low cell-specific and surface passivation-dependent toxicity of these NMs (e.g., [4][5][6][7][8][9]). However, in a study performed in a plant model Arabidopsis thaliana (L.), the negative impacts of CDs exposure on root elongation were observed for higher tested doses, suggesting phytotoxicity in this organism [13]. In that study, a comprehensive genomics analysis using next generation sequencing (NGS) was conducted.…”

Section: Discussionmentioning

confidence: 99%

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The Differential Effect of Carbon Dots on Gene Expression and DNA Methylation of Human Embryonic Lung Fibroblasts as a Function of Surface Charge and Dose

Šíma

Vrbová

Závodná

et al. 2020

IJMS

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This study presents a toxicological evaluation of two types of carbon dots (CD), similar in size (<10 nm) but differing in surface charge. Whole-genome mRNA and miRNA expression (RNAseq), as well as gene-specific DNA methylation changes, were analyzed in human embryonic lung fibroblasts (HEL 12469) after 4 h and 24 h exposure to concentrations of 10 and 50 µg/mL (for positive charged CD; pCD) or 10 and 100 µg/mL (for negative charged CD, nCD). The results showed a distinct response for the tested nanomaterials (NMs). The exposure to pCD induced the expression of a substantially lower number of mRNAs than those to nCD, with few commonly differentially expressed genes between the two CDs. For both CDs, the number of deregulated mRNAs increased with the dose and exposure time. The pathway analysis revealed a deregulation of processes associated with immune response, tumorigenesis and cell cycle regulation, after exposure to pCD. For nCD treatment, pathways relating to cell proliferation, apoptosis, oxidative stress, gene expression, and cycle regulation were detected. The expression of miRNAs followed a similar pattern: more pronounced changes after nCD exposure and few commonly differentially expressed miRNAs between the two CDs. For both CDs the pathway analysis based on miRNA-mRNA interactions, showed a deregulation of cancer-related pathways, immune processes and processes involved in extracellular matrix interactions. DNA methylation was not affected by exposure to any of the two CDs. In summary, although the tested CDs induced distinct responses on the level of mRNA and miRNA expression, pathway analyses revealed a potential common biological impact of both NMs independent of their surface charge.

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Section: Discussionmentioning

confidence: 99%

“…Next generation sequencing of whole genome gene expression changes, after exposure to carbon dots, have so far only been studied in plants. The deregulation of genes responsible for oxidative stress, photosynthetic processes, and the osmotic potential, was observed in Arabidopsis thaliana treated with 3 nm CDs [13].…”

Section: Introductionmentioning

confidence: 99%

The Differential Effect of Carbon Dots on Gene Expression and DNA Methylation of Human Embryonic Lung Fibroblasts as a Function of Surface Charge and Dose

Šíma

Vrbová

Závodná

et al. 2020

IJMS

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“…In 7-day old A. thaliana plants grown on artificial media spiked with 125 and 1000 mg/l carbon nanodots (C-dots) for 7 days, growth was significantly inhibited compared to control and activated-carbon treatments. The metabolite profiling suggested increased accumulation of carbohydrates (melezitose, ribulose-5-phosphate, d-turanose, talose, d-glucopyranoside, inositol) in A. thaliana roots and shoots, which indicates impact on the carbohydrate metabolism and cell wall stress (Chen et al, 2018). In the Cdot exposed A. thaliana tissues, overaccumulation of various alkaloids, lignan, carotenoids, flavonoids, amino acids (Gly, Val, and homoserine (Hse)), organic acids (ethenedecanoic acids, 2-ketoadipic acid, hexacosinic acid, terephthalic acid, butyric acid, carboxylic acid), and fatty acids (heptadecanoic acid and octadecatrienoic acid) were noted, indicating activation of defense response.…”

Section: Carbon -Based Enmsmentioning

confidence: 98%

“…Integration of multiomics in plants provides a comprehensive knowledge on the regulatory mechanism at multiple subcellular organization levels in response to an external stimulus. Although the number of plant studies employing individual omic techniques to identify key biomolecules in response to ENMs is increasing, only a few studies have integrated different omics (Chen et al, 2018;Majumdar et al, 2019;Salehi et al, 2018;Zhao, Zhang, Wang, et al, 2019). It is critical to integrate the response at all levels, including metabolome, proteome and transcriptome to identify the molecular underpinnings that regulate the metabolic pathways in response to ENMs, which eventually is expressed in the phenotype.…”

Section: Integration Of Multiomicsmentioning

confidence: 99%

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Omics to address the opportunities and challenges of nanotechnology in agriculture

Majumdar

Keller

2020

Critical Reviews in Environmental Science and Technology

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Increasing global food demand, and risks associated with climate change and agrochemicals demand novel and sustainable agricultural practices to improve crop yield and quality. Various strategies using nanotechnology have been explored widely to provide solutions. However, their application in the food industry is constrained by limited understanding of nanomaterial safety. Growing interest in the potential of engineered nanomaterials (ENMs) in agricultural applications has also resulted in increased crop exposure, leading to unknown risks to plants, animals and humans. Recently, considerable research has been carried out on overt plant response to ENMs; however, conclusive mechanistic information is lacking. With advancements in hyphenated analytical techniques, research on ENM-biota interactions has witnessed a paradigm shift from low-throughput, single end-point bioassays to high-throughput, discovery-oriented omic tools, including transcriptomics, proteomics and metabolomics. This review summarizes the pioneering studies utilizing omics in plants exposed to ENMs to explain phenotypic expressions and elucidate associated biological pathways. Advantages and challenges of current analytical techniques employed in proteomics and metabolomics are also discussed. We acknowledge the need for integration and application of multiomics to identify sensitive biomarkers in plants in response to ENMs, and provide mechanistic insights, in order to design enhanced and safer nano-enabled products for agriculture.

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Influence of Luminescent Nanomaterials on Plant Growth and Development

Han

et al. 2021

ChemNanoMat

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As an emerging research hotspot in nanotechnology, luminescent nanomaterials (LNMs) have attracted much attention due to their unique properties. Meanwhile, their potential release into the environment has also been investigated by researchers. Plants, as an essential part of the ecosystem, are the starting point of bioaccumulation. Thus, to understand the interaction between LNMs and plants would be one of the critical aspects of risk assessment. Currently, there are still very few reports in this field, where a potential guidance is urgently necessary. Bearing this in mind, herein, based on our own research experience, we summarize the effects of four typical kinds of LNMs (quantum dots, carbon dots, upconversion nanoparticles, and metal nanoclusters) on plant growth and development, including the transport of materials in plants and their toxicity to plants, as well as the according experimental techniques. Finally, the current challenges and prospects are pointed out, thereby hoping to provide a timely fundamental guidance for future research.

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Phenotypic, transcriptional, physiological and metabolic responses to carbon nanodot exposure inArabidopsis thaliana(L.)

Abstract: In this study, we systematically investigated the fate and phytotoxicity of carbon nanodots (C-dots, about 3 nm) inArabidopsis thaliana(Arabidopsis), as well as the underlying potential mechanisms, by integrating transcriptomic, physiological and metabolomic techniques.

Cited by 39 publications

References 64 publications

The Differential Effect of Carbon Dots on Gene Expression and DNA Methylation of Human Embryonic Lung Fibroblasts as a Function of Surface Charge and Dose

The Differential Effect of Carbon Dots on Gene Expression and DNA Methylation of Human Embryonic Lung Fibroblasts as a Function of Surface Charge and Dose

Omics to address the opportunities and challenges of nanotechnology in agriculture

Influence of Luminescent Nanomaterials on Plant Growth and Development

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