To ensure nutritional security of rapidly increasing population, research interest has revitalized in determining the nutritional quality traits of staple food crops, especially wheat. Besides higher yield potential, research gaps exist regarding nutritional quality assessment of promising wheat genotypes grown under the Mediterranean climate. A field study was conducted to determine the relationship between yield components and quality characteristics of 22 bread wheat genotypes using the SPAD meter, GreenSeeker (NDVI), and CM-1000 chlorophyll meter at different growing stages (Stem elongation, Heading, Anthesis and Milk stage). The recorded findings revealed that G-41 genotype surpassed the rest of bread wheat genotypes by recording the maximum grain yield, whereas G-60 genotype exhibited the highest protein and wet gluten content. Among response variables, SPAD and NDVI values at the heading stage and CM-1000 values at the milk stage were found to be statistically insignificant. According to the correlation and biplot analysis, a significant positive correlation was found between the SPAD values measured for the stem elongation, anthesis and milk stage and yield and quality characteristics. Significant positive correlations were found between the NDVI values at the stem elongation, anthesis, and milk stages and the yield components, and between the CM-1000 value at the heading stage and the grain yield.