Four experiments using broiler chicks fed buffalo gourd seed (BGS) in practical-type rations were conducted. The BGS was substituted for part of the soybean meal under different dietary regimens, namely: 1) all plant ration, 2) meat and bone meal-supplemented ration, 3) fish meal and meat and bone meal-supplemented ration. Parameters of response were body weight gain, feed consumption, feed efficiency, mortality, and pancreas weights. Trypsin inhibitor activity of BGS was determined.Results showed that when BGS was incorporated at levels exceeding 5% of the diet, there was a reduction in both body weight gain and feed consumption in two out of four experiments conducted. Regression analysis of the data showed a significant negative linear effect on body weight gain in all experiments when BGS exceeded 5% of the diet. Growth depression observed was mainly the result of reduced feed intake rather than lower feed utilization. Trypsin inhibitor does not seem to be a major antinutritional factor in BGS as no hypertrophy of the pancreas was observed and measures of trypsin inhibitor activity of toasted soybean meal and BGS were not found to be significantly different.(Key words: buffalo gourd seed, dietary regimens, starter rations, trypsin inhibitor activity, broilers)