Phenotypic variation in somaclones of <i>Paspalum dilatatum</i> and their seedling offspring

Davies, LJ; Cohen, Daniel

doi:10.4141/cjps92-093

Cited by 16 publications

(18 citation statements)

References 14 publications

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“…In the SC3 generation, 76-92% of the seedlings were similar to their maternal parent, the remainder being off-types [12]. In 4 of the 5 SC3 families, (seedlings of C39 /1, /4, /5 and / 10) two maternal seedlings and one off-type seedling were selected for study.…”

Section: Methodsmentioning

confidence: 99%

“…Seedlings of Raki and the primary somaclones (SC1) were grown in the field for growth studies for two seasons [12]. Seedlings of the SC 2 generation were germinated in a greenhouse and transplanted into the field for observation over two seasons.…”

Section: Methodsmentioning

confidence: 99%

“…This variation was unexpected as it was anticipated that the somaclones would produce uniform SC2 offspring by apomixis because they were derived from an apomictic donor plant. Davies and Cohen(1992) suggested that the unexpected variation in SC2 seedlings could have been due to a breakdown in the apomixic pathway to seed production in the somaclones, and that SC2 seed may have arisen by a sexual pathway. If sexual reproduction in the somaclones can be proved it follows that tissue culture is a means of breaking ap omixis in P. dilatatum.…”

Section: Introductionmentioning

confidence: 99%

“…In Paspalum dilatatum cv Raki Davies and Cohen(1992) regenerated a large number of plants (somaclones) from embryogenic callus induced on the scutellum of immature embryos. Extensive phenotypic variation in vigor, growth habit, leaf width, and leaf chlorophyll concentration were exhibited by the somaclones obtained (SC1 generation) and most had greatly reduced seed viability compared with Raki.…”

Section: Introductionmentioning

confidence: 99%

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Variation in chromosome number in the seedling progeny of a somaclone of Paspalum dilatatum

Davies

Cohen

et al. 1994

Cell Res

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The somaclone, C39, derived by tissue culture from the obligate apomict Paspalum dilatatum cv Raki (2n=50), had 50 chromosomes and a karyotype apparently identical to Raki. SC2 seedlings of C39 showed a high degree of phenotypic variation which was often associated with increased chromosome numbers, but some of the variant seedlings were karyotypically indistinguishable from Raki or C39. Plants with increased chromosome numbers exhibited a high degree of intraplant chromosome variation (aneusomaty). In one of the SC2 seedlings, the chromosome number of root tip cells varied from 58 to 82 and in several other seedlings the range was more than 10. The results suggested that the ability to form seed apomictically was much reduced in C39 and that this plant showed some capacity for sexual reproduction and the resulting seedlings, with a chromosome number of about 70, were genetically unstable. Of 11 SC2 seedlings examined cytologically, 6 did not produce any viable seed. Seedlings grown from seed of the remaining 5 plants showed that aneusomaty persisted in the SC3 generation. SC3 seedlings which were phenotypically similar to their maternal parent showed a similar range of chromosome numbers to that parent. Some of the SC3 seedlings exhibited an even wider range of chromosome numbers (e.g.56-136), and these plants were all dwarfs.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Variation in chromosome number in the seedling progeny of a somaclone of Paspalum dilatatum

Davies

Cohen

et al. 1994

Cell Res

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“…Bajaj et al, (1981) observed changes in leaf shape in Panicum somaclones. Davies and Cohen (1992) evaluated variation in dallisgrass somaclones. Dwarfing, poor vigour, and floral abnormalities were seen in most regenerated plants.…”

Section: Somaclonal Variation In Forage Grassesmentioning

confidence: 99%