Phenotypic Variations in the Behavior of Sip1 Knockout Mice

Abstract

The aim of the study was the behavioral phenotyping of mice homo-and heterozygous for the Sip1 gene, which plays an important role in the development of the mammalian cerebral cortex.Materials and Methods. The study was performed on mice hetero-and homozygous for the Sip1 gene; these animal models were developed using the Cre recombination method. At an age of 20-30 days, all animals were exposed to a high-intensity sound to identify predisposition to audiogenic epilepsy. At an age of two months, the males were tested for their general physical health and behavioral phenotypes. The tests included a neurological and sensorimotor assessment, an evaluation of anxiety using the light-dark test, a study on locomotion and general exploration in the open field test, the acoustic startle response and prepulse inhibition, social activity in the Crawley's test and the learning ability as scored by the conditioned reflex of passive avoidance.Results. Mice homozygous for the Sip1 gene never reached the age of two months. In heterozygous mice, a higher occurrence of hind limb extension reflex abnormalities, an increased level of anxiety in the light-dark test, and a decrease in social activity in the Crawley's test were found.Conclusion. The presence of a mutant allele of the Sip1 gene leads to neurologic disorders, an increase in anxiety and a decrease in the social activity of the animals.